Transfer of antibiotic resistance determinants between lactobacilli isolates from the gastrointestinal tract of chicken
In: Beneficial MicrobesSearch for other papers by F. Vieira de Souza in
Current site
Google Scholar
Search for other papers by R. Roque in
Current site
Google Scholar
Search for other papers by J.L. Silva Moreira in
Current site
Google Scholar
Search for other papers by M. Resende de Souza in
Current site
Google Scholar
Search for other papers by J.R. Nicoli in
Current site
Google Scholar
Search for other papers by E. Neumann in
Current site
Google Scholar
cantini@icb.ufmg.br
Search for other papers by Á. Cantini Nunes in
Current site
Google Scholar
Abstract
The aim of this study was to assess the potential horizontal transfer of genetic traits for antibiotic resistance between lactobacilli isolated from the chicken gut, both in vitro and in vivo. Thirty-seven Lactobacillus spp. strains isolated from the gizzard, small and large intestines and caeca of free-range broiler chickens showed multi-drug resistance as assessed by disc diffusion assays. The minimum inhibitory concentration (MIC) for vancomycin, tetracycline, erythromycin and chloramphenicol was determined in De Man, Rogosa and Sharpe broth in a microplate assay. Almost all the lactobacilli isolates were resistant to vancomycin (except strains belonging to the Lactobacillus acidophilus group) and to tetracycline (MIC≥128 μg/ml). Only five strains were resistant to erythromycin, and six to chloramphenicol. The transfer rate in filter mating experiments performed using L. acidophilus strain 4M14E (EmR), Lactobacillus vaginalis strain 5M14E (CmR), Lactobacillus salivarius strain 5C14C (EmR), and the 4G14L and 3C14C strains of Lactobacillus reuteri (CmR) showed a frequency of approximately 1×104 cfu/ml of double-resistant transconjugants for the different combinations. The exception was the L. salivarius 5C14C (EmR) and L. vaginalis 5M14E (CmR) mating combination, which produced no transconjugants. In vivo experiments performed in gnotobiotic mice by mating L. acidophilus 4M14E (EmR) with L. reuteri 3C14C (CmR), L. reuteri 4G14L (CmR) or L. vaginalis 5M14E (CmR) resulted in transconjugants at 3.95±0.29, 3.16±0.33, and 4.55±1.52 log10 cfu/g of faeces, respectively. Taken together, these data suggest that genetic exchange may occur between native bacterial strains within the gastrointestinal tract of chickens, which might maintain a dynamic gene pool conferring antibiotic resistance upon indigenous microbiota components, even in the absence of the pathogens. This possibility must be taken into account as a complementary criterion when lactobacilli are screened for probiotic use.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Andersson, D.I. and Hughes, D., 2010. Antibiotic resistance and its cost: is it possible to reverse resistance? Nature Reviews Microbiology 8: 260-271.
'Antibiotic resistance and its cost: is it possible to reverse resistance? ' () 8 Nature Reviews Microbiology : 260 -271.
-
Ahn, C., Collins-Thompson, D., Duncan, C. and Stiles, M.E., 1992. Mobilization and location of the genetic determinant of chloramphenicol resistance from Lactobacillus plantarum caTC2R. Plasmid 27: 169-176.
'Mobilization and location of the genetic determinant of chloramphenicol resistance from Lactobacillus plantarum caTC2R ' () 27 Plasmid : 169 -176.
-
Barkay, T. and Smets, B.F., 2005. Horizontal gene flow in microbial communities. American Society for Microbiology News 71: 412-419.
'Horizontal gene flow in microbial communities ' () 71 American Society for Microbiology News : 412 -419.
-
Charteris, W.P., Kelly, P.M., Morelli, L. and Collins, J.K., 1998. Antibiotic susceptibility of potentially probiotic Lactobacillus species. Journal of Food Protection 61: 1636-1643.
'Antibiotic susceptibility of potentially probiotic Lactobacillus species ' () 1 Journal of Food Protection : 1636 -1643.
-
Chin, S.C., Abdullah, N., Siang, T.W. and Wan, H.Y., 2005. Plasmid profiling and curing of Lactobacillus strains isolated from the gastrointestinal tract of chicken. Journal of Microbiology 43: 251-256.
'Plasmid profiling and curing of Lactobacillus strains isolated from the gastrointestinal tract of chicken ' () 43 Journal of Microbiology : 251 -256.
-
Clewell, D.B., Flannagan, S.E. and Jaworski, D.D., 1995. Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons. Trends in Microbiology 3: 229-236.
'Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons ' () 3 Trends in Microbiology : 229 -236.
-
Colégio Brasileiro de Experimentação Animal (COBEA), 2006. Legislação e ética. Available at: http://www.cobea.org.br/. http://www.cobea.org.br/
-
Danielsen, M., 2002. Characterization of the tetracycline resistance plasmid pMD5057 from Lactobacillus plantarum 5057 reveals a composite structure. Plasmid 48: 98-103.
'Characterization of the tetracycline resistance plasmid pMD5057 from Lactobacillus plantarum 5057 reveals a composite structure ' () 48 Plasmid : 98 -103.
-
Danielsen, M. and Wind, A., 2003. Susceptibility of Lactobacillus spp. to antimicrobial agents. International Journal of Food Microbiology 82: 1-11.
'Susceptibility of Lactobacillus spp. to antimicrobial agents ' () 82 International Journal of Food Microbiology : 1 -11.
-
Feld, L., Schjørring, S., Hammer, K., Licht, T.R., Danielsen, M., Krogfelt, K. and Wilcks, A., 2008. Selective pressure affects transfer and establishment of a Lactobacillus plantarum resistance plasmid in the gastrointestinal environment. Journal of Antimicrobial Chemotherapy 61: 845-852.
'Selective pressure affects transfer and establishment of a Lactobacillus plantarum resistance plasmid in the gastrointestinal environment ' () 1 Journal of Antimicrobial Chemotherapy : 845 -852.
-
Fons, M., Hege, T., Ladire, M., Raibaud, P., Ducluzeau, R. and Maguin, E., 1997. Isolation and characterization of a plasmid from Lactobacillus fermentum conferring erythromycin resistance. Plasmid 37: 199-203.
'Isolation and characterization of a plasmid from Lactobacillus fermentum conferring erythromycin resistance ' () 37 Plasmid : 199 -203.
-
Gevers, D., Huys, G. and Swings, J., 2003. In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other Gram-positive bacteria. FEMS Microbiology Letters 225: 125-130.
'In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other Gram-positive bacteria ' () 225 FEMS Microbiology Letters : 125 -130.
-
Jacobsen, L., Wilcks, A., Hammer, K., Huys, G., Gevers, D. and Andersen, S.R., 2007. Horizontal transfer of tet(M) and erm(B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats. FEMS Microbiology Ecology 59: 158-166.
'Horizontal transfer of tet(M) and erm(B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats ' () 59 FEMS Microbiology Ecology : 158 -166.
-
Jain, R., Rivera, M.C. and Lake, J.A., 1999. Horizontal gene transfer among genomes: the complexity hypothesis. Proceedings of the National Academy of Sciences of the USA 96: 3801-3806.
, , .
-
Langella, P., LeLoir, Y., Echlich, S.D. and Gruss, A., 1993. Efficient plasmid mobilization by pIP501 in Lactococcus lactis subsp. lactis. Journal of Bacteriology 18: 5806-5813.
'Efficient plasmid mobilization by pIP501 in Lactococcus lactis subsp. lactis ' () 18 Journal of Bacteriology : 5806 -5813.
-
Leite, A.A., Nardi, R.M., Nicoli, J.R., Chartone-Souza, E. and Nascimento, A.M., 2005. Carica papaya seed macerate as inhibitor of conjugative R plasmid transfer from Salmonella typhimurium to Escherichia coli in vitro and in the digestive tract of gnotobiotic mice. Journal of General and Applied Microbiology 51: 21-26.
'Carica papaya seed macerate as inhibitor of conjugative R plasmid transfer from Salmonella typhimurium to Escherichia coli in vitro and in the digestive tract of gnotobiotic mice ' () 51 Journal of General and Applied Microbiology : 21 -26.
-
Moreira, J.S., Mota, R.M., Horta, M.F., Teixeira, S.M.R., Neumann, E., Nicoli, J.R. and Nunes, A.C., 2005. Identification to the species level of Lactobacillus isolated in probiotic prospecting studies by 16S-23S rRNA restriction profiling. BMC Microbiology 5: 15.
'Identification to the species level of Lactobacillus isolated in probiotic prospecting studies by 16S-23S rRNA restriction profiling ' () 5 BMC Microbiology : 15.
-
Pleasants, J.R., 1974. Gnotobiotics. In: Melby, E.C. and Altman, N.H. (eds.) Handbook of laboratory animal science. CRC Press, Cleveland, OH, USA, pp. 119-174.
Handbook of laboratory animal science , () 119 -174.
-
Salyers, A.A., Shoemaker, N.B., Stevens, A.M. and Li, L.Y., 1995. Conjugative transposons: an unusual and diverse set of gene transfer elements. Microbiology Reviews 4: 579-590.
'Conjugative transposons: an unusual and diverse set of gene transfer elements ' () 4 Microbiology Reviews : 579 -590.
-
Tannock, G.W., 1987. Conjugal transfer of plasmid pAM beta1 in Lactobacillus reuteri and between lactobacilli and Enterococcus faecalis. Applied and Environmental Microbiology 53: 2693-2695.
'Conjugal transfer of plasmid pAM beta1 in Lactobacillus reuteri and between lactobacilli and Enterococcus faecalis ' () 53 Applied and Environmental Microbiology : 2693 -2695.
-
Tannock, G.W., Luchansky, J.B., Miller, L., Connell, H., Thodeandersen, S., Mercer, A.A. and Kalenhammer, T.R., 1994. Molecular characterization of a plasmid borne (pGT633) erythromycin resistance determinant (ermGT) from Lactobacillus reuteri 100-63. Plasmid 31: 60-71.
'Molecular characterization of a plasmid borne (pGT633) erythromycin resistance determinant (ermGT) from Lactobacillus reuteri 100-63 ' () 31 Plasmid : 60 -71.
-
Vescovo, M., Morelli, L. and Bottazzi, V., 1982. Drug resistance plasmids in Lactobacillus acidophilus and Lactobacillus reuteri. Applied and Environmental Microbiology 43: 50-56.
'Drug resistance plasmids in Lactobacillus acidophilus and Lactobacillus reuteri ' () 43 Applied and Environmental Microbiology : 50 -56.
-
Wang, T.T. and Lee, B.H., 1997. Plasmids in Lactobacillus. Critical Reviews in Biotechnology 17: 227-272.
'Plasmids in Lactobacillus ' () 17 Critical Reviews in Biotechnology : 227 -272.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 388 | 120 | 13 |
| Full Text Views | 95 | 32 | 0 |
| PDF Views & Downloads | 22 | 7 | 0 |
Transfer of antibiotic resistance determinants between lactobacilli isolates from the gastrointestinal tract of chicken
In: Beneficial MicrobesSearch for other papers by F. Vieira de Souza in
Current site
Google Scholar
PubMed
Search for other papers by R. Roque in
Current site
Google Scholar
PubMed
Search for other papers by J.L. Silva Moreira in
Current site
Google Scholar
PubMed
Search for other papers by M. Resende de Souza in
Current site
Google Scholar
PubMed
Search for other papers by J.R. Nicoli in
Current site
Google Scholar
PubMed
Search for other papers by E. Neumann in
Current site
Google Scholar
PubMed
cantini@icb.ufmg.br
Search for other papers by Á. Cantini Nunes in
Current site
Google Scholar
PubMed
Abstract
The aim of this study was to assess the potential horizontal transfer of genetic traits for antibiotic resistance between lactobacilli isolated from the chicken gut, both in vitro and in vivo. Thirty-seven Lactobacillus spp. strains isolated from the gizzard, small and large intestines and caeca of free-range broiler chickens showed multi-drug resistance as assessed by disc diffusion assays. The minimum inhibitory concentration (MIC) for vancomycin, tetracycline, erythromycin and chloramphenicol was determined in De Man, Rogosa and Sharpe broth in a microplate assay. Almost all the lactobacilli isolates were resistant to vancomycin (except strains belonging to the Lactobacillus acidophilus group) and to tetracycline (MIC≥128 μg/ml). Only five strains were resistant to erythromycin, and six to chloramphenicol. The transfer rate in filter mating experiments performed using L. acidophilus strain 4M14E (EmR), Lactobacillus vaginalis strain 5M14E (CmR), Lactobacillus salivarius strain 5C14C (EmR), and the 4G14L and 3C14C strains of Lactobacillus reuteri (CmR) showed a frequency of approximately 1×104 cfu/ml of double-resistant transconjugants for the different combinations. The exception was the L. salivarius 5C14C (EmR) and L. vaginalis 5M14E (CmR) mating combination, which produced no transconjugants. In vivo experiments performed in gnotobiotic mice by mating L. acidophilus 4M14E (EmR) with L. reuteri 3C14C (CmR), L. reuteri 4G14L (CmR) or L. vaginalis 5M14E (CmR) resulted in transconjugants at 3.95±0.29, 3.16±0.33, and 4.55±1.52 log10 cfu/g of faeces, respectively. Taken together, these data suggest that genetic exchange may occur between native bacterial strains within the gastrointestinal tract of chickens, which might maintain a dynamic gene pool conferring antibiotic resistance upon indigenous microbiota components, even in the absence of the pathogens. This possibility must be taken into account as a complementary criterion when lactobacilli are screened for probiotic use.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 388 | 120 | 13 |
| Full Text Views | 95 | 32 | 0 |
| PDF Views & Downloads | 22 | 7 | 0 |
