Postbiotics: what are they?
äºBeneficial MicrobesDepartment of General Hygiene, I.M. Sechenov First Moscow State Medical University, Trubetskaya Street 8, Building. 2, Moscow 119991, Russia
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ProBacLab, LaboratoÌrio de Microbiologia de Alimentos, Departamento de Alimentos e NutriçaÌo Experimental, Food Research Center, Faculdade de CieÌncias FarmaceÌuticas, Universidade de SaÌo Paulo, SaÌo Paulo (SP), Brazil
CISASÂ â Center for Research and Development in Agrifood Systems and Sustainability, Instituto PoliteÌcnico de Viana do Castelo, 4900-347 Viana do Castelo, Portugal
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Abstract
Although several internationally recognised scientific groups and non-governmental organisations have attempted to define âpostbiotics,â there remains no consensus. Leading experts have highlighted persistent problems of terminological inconsistency, lack of standardisation, and unclear boundaries between related concepts. What is needed is a comprehensive analysis that integrates existing definitions with considerations of manufacturing processes, mechanisms of action, and clinical applications. Ultimately, progress requires harmonisation of terminology to ensure comparability across studies and to provide a solid foundation for both academic and industry development.
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Alekseeva, A.I., Ye, K., Mentani, J., Wolkers-Rooijackers, J.C.M., Smid, E.J. and Schoustra, S.E., 2025. Traditional fermented milk product from Zambia shifts the gut microbiota to healthier metabolism in a simulated SHIME® model system. Beneficial Microbes. Online, ahead of print. https://doi.org/10.1163/18762891-bja00068
-
Bourrie, B.C.T., Forgie, A.J., Ju, T., Richard, C., Cotter, P.D. and Willing, B.P., 2022. Consumption of the cell-free or heat-treated fractions of a pitched kefir confers some but not all positive impacts of the corresponding whole kefir. Frontiers in Microbiology 13: 1056526. https://doi.org/10.3389/fmicb.2022.1056526
-
Boyte, M.E., Benkowski, A., Pane, M. and Shehata, H.R., 2023. Probiotic and postbiotic analytical methods: a perspective of available enumeration techniques. Frontiers in Microbiology 14: 1304621. https://doi.org/10.3389/fmicb.2023.1304621
-
Chernov, V.M., Chernova, O.A., Markelova, M.I. and Trushin, M.V., 2024. Probiotics under selective pressure: novel insights and biosafety challenge. Archives of Razi Institute 79(6): 1165-1182. https://doi.org/10.32592/ARI.2024.79.6.1165
-
Cotton, C., 2024. The Essays of Michel de Montaigne. Online Book 3, Chapter 13: on Experience. https://hyperessays.net/essays/on-experience/ Accessed on: 2025-07-11.
-
Della Volpe, A., De Luca, P., De Lucia, A., Martines, F., Piroli, P., DâAscanio, L., Camaioni, A., La Mantia, I. and Di Stadio, A., 2022. Single-center-single-blinded clinical trial to evaluate the efficacy of a nutraceutical containing Boswellia serrata, bromelain, zinc, magnesium, honey, tyndallized Lactobacillus acidophilus and casei to fight upper respiratory tract infection and otitis media. Healthcare 10(8): 1526. https://doi.org/10.3390/healthcare10081526
-
Di Vincenzo, S., Ferraro, M., Taverna, S., Malizia, V., Buscetta, M., Cipollina, C., Lazzara, V., Pinto, P., Bassano, M., La Grutta, S. and Pace, E., 2023. Tyndallized bacteria preferentially induce human macrophage M1 polarization: an effect useful to balance allergic immune responses and to control infections. Antibiotics 12(3): 571. https://doi.org/10.3390/antibiotics12030571
-
Di Vincenzo, S., Di Sano, C., DâAnna, C., Ferraro, M., Malizia, V., Bruno, A., Cristaldi, M., Cipollina, C., Lazzara, V., Pinto, P., La Grutta, S. and Pace, E., 2024. Tyndallized bacteria prime bronchial epithelial cells to mount an effective innate immune response against infections. Human Cell 37(4): 1080-1090. https://doi.org/10.1007/s13577-024-01080-z
-
DomıÌnguez-Maqueda, M., Espinosa-RuıÌz, C., Esteban, M.AÌ., AlarcoÌn, F.J., Tapia-Paniagua, S.T., Balebona, M.C. and MorinÌigo, M.AÌ., 2025. An ex vivo approach in European seabass leucocytes supports the in vitro regulation by postbiotics of aip56 gene expression of Photobacterium damselae subsp. piscicida. Probiotics and Antimicrobial Proteins 17(4): 2097-2111. https://doi.org/10.1007/s12602-024-10255-x
-
Dong, Y., Wu, X., Zhang, Y., Hu, A., Zhou, Q., Yue, X., Liu, Z. and Li, M., 2025. The role of probiotics in modulating the gut microbiome in Alzheimerâs disease: a review. Foods 14(9): 1531. https://doi.org/10.3390/foods14091531
-
Foucault, M., 1972. The archaeology of knowledge (world of man). Published by Pantheon, 1972. ISBN 10: 0394471180 / ISBN 13: 9780394471181.
-
Gibson, G.R., Hutkins, R., Sanders, M.E., Prescott, S.L., Reimer, R.A., Salminen, S.J., Scott, K., Stanton, C., Swanson, K.S., Cani, P.D., Verbeke, K. and Reid, G., 2017. Expert consensus document: the International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nature Reviews Gastroenterology & Hepatology 14(8): 491-502. https://doi.org/10.1038/nrgastro.2017.75
-
Gorzelanna, Z., Mamrot, A., Będkowska, D., Bubak, J. and Miszczak, M., 2025. Exploring the potential of novel animal-origin probiotics as key players in one health: opportunities and challenges. International Journal of Molecular Sciences 26(11): 5143. https://doi.org/10.3390/ijms26115143
-
Guan, L., Hu, A., Ma, S., Liu, J., Yao, X., Ye, T., Han, M., Yang, C., Zhang, R., Xiao, X. and Wu, Y., 2024. Lactiplantibacillus plantarum postbiotic protects against Salmonella infection in broilers via modulating NLRP3 inflammasome and gut microbiota. Poultry Science 103(4): 103483. https://doi.org/10.1016/j.psj.2024.103483
-
Guglielmetti, S., Boyte, M.-E., Smith, C.L., Ouwehand, A.C., Paraskevakos, G. and Younes, J.A., 2025. Commercial and regulatory frameworks for postbiotics: an industry-oriented scientific perspective for non-viable microbial ingredients conferring beneficial physiological effects. Trends in Food Science & Technology 163: 105130. https://doi.org/10.1016/j.tifs.2025.105130
-
Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein, D.J., Pot, B., Morelli, L., Canani, R.B., Flint, H.J., Salminen, S., Calder, P.C. and Sanders, M.E., 2014. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology & Hepatology 11(8): 506-514. https://doi.org/10.1038/nrgastro.2014.66
-
Kuru-Yaşar, R. and Ãstün-Aytekin, Ã., 2024. The crucial roles of diet, microbiota, and postbiotics in colorectal cancer. Current Nutrition Reports 13: 126-151. https://doi.org/10.1007/s13668-024-00525-z
-
Lee, D., Goh, T.W., Kang, M.G., Choi, H.J., Yeo, S.Y., Yang, J., Huh, C.S., Kim, Y.Y. and Kim, Y., 2022. Perspectives and advances in probiotics and the gut microbiome in companion animals. Journal of Animal Science and Technology 4(2): 197-217. https://doi.org/10.5187/jast.2022.e8
-
Lee, N.K., Park, Y.S., Kang, D.K. and Paik, H.D., 2023. Paraprobiotics: definition, manufacturing methods, and functionality. Food Science and Biotechnology 32(14): 1981-1991. https://doi.org/10.1007/s10068-023-01378-y
-
Liang, B. and Xing, D., 2023. The current and future perspectives of postbiotics. Probiotics and Antimicrobial Proteins 15(6): 1626-1643. https://doi.org/10.1007/s12602-023-10045-x
-
Liang, D., Wu, F., Zhou, D., Tan, B. and Chen, T., 2024. Commercial probiotic products in public health: current status and potential limitations. Critical Reviews in Food Science and Nutrition 64(19): 6455-6476. https://doi.org/10.1080/10408398.2023.2169858
-
Martyniak, A., MedynÌska-Przęczek, A., Wędrychowicz, A., SkoczenÌ, S. and Tomasik, P.J., 2021. Prebiotics, probiotics, synbiotics, paraprobiotics and postbiotic compounds in IBD. Biomolecules 11(12): 1903. https://doi.org/10.3390/biom11121903
-
Matsuzaki, T., Yamazaki, R., Hashimoto, S. and Yokokura, T.J., 1998. The effect of oral feeding of Lactobacillus casei strain Shirota on immunoglobulin E production in mice. Dairy Science 81(1): 48-53. https://doi.org/10.3168/jds.S0022-0302(98)75549-3
-
Mohammadi, R., Moradi, M., Tajik, H. and Molaei, R., 2022. Potential application of postbiotics metabolites from bioprotective culture to fabricate bacterial nanocellulose based antimicrobial packaging material. International Journal of Biological Macromolecules 220: 528-536. https://doi.org/10.1016/j.ijbiomac.2022.08.108
-
Mora-SaÌnchez, B., BalcaÌzar, J.L. and PeÌrez-SaÌnchez, T., 2020. Effect of a novel postbiotic containing lactic acid bacteria on the intestinal microbiota and disease resistance of rainbow trout (Oncorhynchus mykiss). Biotechnology Letters 42(10): 1957-1962. https://doi.org/10.1007/s10529-020-02919-9
-
Neumann, E., Oliveira, M.A., Cabral, C.M., Moura, L.N., Nicoli, J.R., Vieira, E.C., Cara, D.C., Podoprigora, G.I. and Vieira, L.Q., 1998. Monoassociation with Lactobacillus acidophilus UFV-H2b20 stimulates the immune defense mechanisms of germfree mice. Brazilian Journal of Medical and Biological Research 31(12): 1565-1573. https://doi.org/10.1590/s0100-879x1998001200009
-
Ramazanidoroh, R., Hosseininezhad, M., Shahrampour, D. and Wu, X., 2024. Edible packaging as a functional carrier of prebiotics, probiotics, and postbiotics to boost food safety, quality, and shelf life. Probiotics and Antimicrobial Proteins 16(4): 1327-1347. https://doi.org/10.1007/s12602-023-10110-5
-
Salminen, S., Collado, M.C., Endo, A., Hill, C., Lebeer, S., Quigley, E.M.M., Sanders, M.E., Shamir, R., Swann, J.R., Szajewska, H. and Vinderola, G., 2021. The International Scientific Association of Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of postbiotics. Nature Reviews Gastroenterology & Hepatology 18(9): 649-667. https://doi.org/10.1038/s41575-021-00440-6 Erratum in: Nature Reviews in Gastroenterology and Hepatology 2021, 18(9): 671. https://doi.org/10.1038/s41575-021-00481-x Erratum in: Nature Reviews in Gastroenterology and Hepatology 2022, 19(8): 551. https://doi.org/10.1038/s41575-022-00628-4
-
Toushik, S.H., Kim, K., Park, S.H., Park, J.H., Ashrafudoulla, M., Ulrich, M.S.I., Mizan, M.F.R., Hossain, M.I., Shim, W.B., Kang, I. and Ha, S.D., 2022. Prophylactic efficacy of Lactobacillus curvatus B67-derived postbiotic and quercetin, separately and combined, against Listeria monocytogenes and Salmonella enterica ser. Typhimurium on processed meat sausage. Meat Science 197: 109065. https://doi.org/10.1016/j.meatsci.2022.109065
-
Vinderola, G. and Audisio, M.C., 2024. Metabiotics: do we need a new definition? Revista Argentina de Microbiologia 56(3): 203-204. https://doi.org/10.1016/j.ram.2024.09.002
-
Vinderola, G., Sanders, M.E., Cunningham, M. and Hill, C., 2024. Frequently asked questions about the ISAPP postbiotic definition. Frontiers in Microbiology 14: 1324565. https://doi.org/10.3389/fmicb.2023.1324565
-
Vinderola, G., Sanders, M.E. and Salminen, S., 2022. The concept of postbiotics. Foods 11(8): 1077. https://doi.org/10.3390/foods11081077
-
Vinderola, G., Benkowski, A., Bernardeau, M., Chenoll, E., Collado, M.C., Cronin, U., Eckhardt, E., Green, J.B., Ipharraguerre, I.R., Kemperman, R., Lacroix, C., Minami, J., Wilkinson, M., Sanders, M.E. and Salminen, S., 2025. Postbiotics: a perspective on their quantification. Frontiers in Nutrition 12: 1582733. https://doi.org/10.3389/fnut.2025.1582733
-
Xu, R., Yu, Y. and Che, T., 2025. Exploring the dark side of probiotics to pursue light: intrinsic and extrinsic risks to be opportunistic pathogens. Current Research in Food Science 10: 101044. https://doi.org/10.1016/j.crfs.2025.101044
-
Zhang, Z., Zhao, J., Zang, J., Peng, C., Lv, L. and Li, Z., 2024. The inhibition mechanism of PostbioYDFF-3 on quality deterioration of refrigerated grass carp fillets from the perspective of endogenous enzyme and microorganisms changes. Food Chemistry 450: 139345. https://doi.org/10.1016/j.foodchem.2024.139345
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| æè¦æµè§æ¬¡æ° | 967 | 967 | 96 |
| å ¨ææµè§æ¬¡æ° | 37 | 37 | 2 |
| PDFä¸è½½æ¬¡æ° | 80 | 80 | 3 |
Postbiotics: what are they?
äºBeneficial MicrobesDepartment of General Hygiene, I.M. Sechenov First Moscow State Medical University, Trubetskaya Street 8, Building. 2, Moscow 119991, Russia
Search for other papers by M.L. Chikindas in
Current site
Google Scholar
PubMed
Search for other papers by L.S. Sichel in
Current site
Google Scholar
PubMed
Search for other papers by I.V. Popov in
Current site
Google Scholar
PubMed
Search for other papers by J.R. Tagg in
Current site
Google Scholar
PubMed
Search for other papers by X. Lu in
Current site
Google Scholar
PubMed
Search for other papers by O.V. Mitrokhin in
Current site
Google Scholar
PubMed
ProBacLab, LaboratoÌrio de Microbiologia de Alimentos, Departamento de Alimentos e NutriçaÌo Experimental, Food Research Center, Faculdade de CieÌncias FarmaceÌuticas, Universidade de SaÌo Paulo, SaÌo Paulo (SP), Brazil
CISASÂ â Center for Research and Development in Agrifood Systems and Sustainability, Instituto PoliteÌcnico de Viana do Castelo, 4900-347 Viana do Castelo, Portugal
Search for other papers by S.D. Todorov in
Current site
Google Scholar
PubMed
Abstract
Although several internationally recognised scientific groups and non-governmental organisations have attempted to define âpostbiotics,â there remains no consensus. Leading experts have highlighted persistent problems of terminological inconsistency, lack of standardisation, and unclear boundaries between related concepts. What is needed is a comprehensive analysis that integrates existing definitions with considerations of manufacturing processes, mechanisms of action, and clinical applications. Ultimately, progress requires harmonisation of terminology to ensure comparability across studies and to provide a solid foundation for both academic and industry development.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 967 | 967 | 96 |
| å ¨ææµè§æ¬¡æ° | 37 | 37 | 2 |
| PDFä¸è½½æ¬¡æ° | 80 | 80 | 3 |
