Feed supplementation with Ligilactobacillus salivarius PS21603 optimises intestinal morphology and gut microbiota composition in weaned piglets
äºBeneficial MicrobesSearch for other papers by I. Cuevas-GoÌmez in
Current site
Google Scholar
Search for other papers by J. de AndreÌs in
Current site
Google Scholar
Search for other papers by N. Cardenas in
Current site
Google Scholar
Search for other papers by I. Espinosa-Martos in
Current site
Google Scholar
Search for other papers by E. JimeÌnez in
Current site
Google Scholar
Abstract
Post-weaning diarrhoea in piglets remains an important cause of economic losses for swine producers. Feed supplementation with probiotics is one of the alternatives to antibiotics used to reduce the impact of such gastrointestinal disease. The aim of the present study was to evaluate the effect of Ligilactobacillus salivarius PS21603 supplementation on the intestinal structure and the gut microbiota composition of weaned piglets. Safety and tolerance of L. salivarius PS21603 were previously evaluated in a 28-days study using 384 weaned piglets (28 ± 2 days old and 7.5 ± 1.5 kg) divided in three treatment groups: T1: Basal diet + L. salivarius PS21603 109 cfu/day, T2: Basal diet + L. salivarius PS21603 107 cfu/day, and T3: Basal diet (control group). For the present study, 16 piglets per treatment group were randomly selected and faecal samples were collected on day 0 (weaning) and 28 of study. At the end of study, three males and three females per treatment were euthanised. Intestinal morphometric values were measured after necropsy. Faecal counts of Escherichia coli were evaluated by culture techniques, and faecal microbiota composition was assessed by high-throughput sequencing. All data were analysed and compared between treatment groups. Supplementation with L. salivarius PS21603 caused an increase in the intestine length of piglets from T1 and in the villous height:crypt ratio of piglets from T2 (P < 0.05) compared to T3 on day 28. According to the Shannon Diversity Index, microbiota diversity increased on day 28 compared to day 0, with no significant differences observed between treatments. The main changes in the relative abundance of bacteria at the phylum, family, and genus levels were observed between different sampling time points. However, piglets from T1 and T2 had lower faecal E. coli counts than T3 on day 28 (P < 0.05). Moreover, supplementation with L. salivarius PS21603 modulated gut microbiota through a more optimal composition, reducing Escherichia and increasing Bifidobacterium relative abundance in piglets from T1 (P < 0.05) from the beginning to the end of the study. Therefore, the strain L. salivarius PS21603 has shown probiotic properties to be used as feed additive in the pig industry, along with good hygiene and farm management practices, for the prevention and/or treatment of post-weaning diarrhoea in piglets.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Alba, C., CastejoÌn, D., Remiro, V., RodrıÌguez, J.M., Sobrino, O.J., de MarıÌa, J., Fumanal, P., Fumanal, A. and Cambero, M.I., 2023. Ligilactobacillus salivarius MP100 as an alternative to metaphylactic antimicrobials in swine: the impact on production parameters and meat composition. Animals 13: 1653. https://doi.org/10.3390/ani13101653
-
Chen, L., Xu, Y., Chen, X., Fang, C., Zhao, L. and Chen, F., 2017. The maturing development of gut microbiota in commercial piglets during the weaning transition. Frontiers in Microbiology 8: 1688. https://doi.org/10.3389/fmicb.2017.01688
-
Cuevas-GoÌmez, I., de AndreÌs, J., Cardenas, N., Espinosa-Martos, I. and JimeÌnez, E., 2022. Safety assessment and characterisation of Ligilactobacillus salivarius PS21603 as potential feed additive for swine. Beneficial Microbes 13: 397-406. https://doi.org/10.3920/BM2022.0020
-
Deng, J., Li, Y., Zhang, J. and Yang, Q., 2013. Co-administration of Bacillus subtilis RJGP16 and Lactobacillus salivarius B1 strongly enhances the intestinal mucosal immunity of piglets. Research in Veterinary Science 94: 62-68. https://doi.org/10.1016/j.rvsc.2012.07.025
-
Di Giancamillo, A., Vitari, F., Savoini, G., Bontempo, V., Bersani, C., DellâOrto, V. and Domeneghini, C., 2008. Effects of orally administered probiotic Pediococcus acidilactici on the small and large intestine of weaning piglets. A qualitative and quantitative micro-anatomical study. Histology Histopathology 23: 651-664. https://doi.org/10.14670/HH-23.651
-
Dibner, J.J. and Richards, J.D., 2005. Antibiotic growth promoters in agriculture: history and mode of action. Poultry Science 84: 634-643. https://doi.org/10.1093/ps/84.4.634
-
Dou, S., Gadonna-Widehem, P., Rome, V., Hamoudi, D., Rhazi, L., Lakhal, L., Larcher, T., Bahi-Jaber, N., Pinon-Quintana, A., Guyonvarch, A., Huërou-Luron, I.L.E. and Abdennebi-Najar, L., 2017. Characterisation of early-life fecal microbiota in susceptible and healthy pigs to post-weaning diarrhoea. PLoS ONE 12: e0169851. https://doi.org/10.1371/journal.pone.0169851
-
Dowarah, R., Verma, A.K. and Agarwal, N., 2017. The use of Lactobacillus as an alternative of antibiotic growth promoters in pigs: a review. Animal Nutrition 3: 1-6. https://doi.org/10.1016/j.aninu.2016.11.002
-
Dubreuil, J.D., Isaacson, R.E. and Schifferli, D.M., 2016. Animal enterotoxigenic Escherichia coli. EcoSal Plus 7. https://doi.org/10.1128/ecosalplus.ESP-0006-2016
-
Fairbrother, J.M., Nadeau, E. and Gyles, C.L., 2005. Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies. Animal Health Research Reviews 6: 17-39. https://doi.org/10.1079/ahr2005105
-
Fouhse, J.M., Zijlstra, R.T. and Willing, B.P., 2016. The role of gut microbiota in the health and disease of pigs. Animal Frontiers 6: 30-36. https://doi.org/10.2527/af.2016-0031
-
Ghareeb, K., Awad, W.A., Mohnl, M., Porta, R., BiarneÌs, M., Böhm, J. and Schatzmayr, G., 2012. Evaluating the efficacy of an avian-specific probiotic to reduce the colonization of Campylobacter jejuni in broiler chickens. Poultry Science 91: 1825-1832. https://doi.org/10.3382/ps.2012-02168
-
Gresse, R., Chaucheyras-Durand, F., Fleury, M.A., Van de Wiele, T., Forano, E. and Blanquet-Diot, S., 2017. Gut microbiota dysbiosis in postweaning piglets: understanding the keys to health. Trends in Microbiology 25: 851-873. https://doi.org/10.1016/j.tim.2017.05.004
-
Guevarra, R.B., Hong, S.H., Cho, J.H., Kim, B.-R., Shin, J., Lee, J.H., Kang, B.N., Kim, Y.H., Wattanaphansak, S., Isaacson, R.E., Song, M. and Kim, H.B., 2018. The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition. Journal of Animal Science and Biotechnology 9: 54. https://doi.org/10.1186/s40104-018-0269-6
-
Guevarra, R.B., Lee, J.H., Lee, S.H., Seok, M.-J., Kim, D.W., Kang, B.N., Johnson, T.J., Isaacson, R.E. and Kim, H.B., 2019. Piglet gut microbial shifts early in life: causes and effects. Journal of Animal Science and Biotechnology 10: 1. https://doi.org/10.1186/s40104-018-0308-3
-
Han, G.G., Lee, J.-Y., Jin, G.-D., Park, J., Choi, Y.H., Kang, S.-K., Chae, B.J., Kim, E.B. and Choi, Y.-J., 2018. Tracing of the fecal microbiota of commercial pigs at five growth stages from birth to shipment. Science Reports 8: 6012. https://doi.org/10.1038/s41598-018-24508-7
-
Helke, K.L., Ezell, P.C., Duran-Struuck, R. and Swindle, M.M., 2015. Biology and diseases of swine. In: Fox, J.G., Anderson, L.C., Otto, G.M., Pritchett-Corning, K.R. and Whary, M.T. (eds.) Laboratory animal medicine. Academic Press, Cambridge, MA, USA, pp. 695-769. https://doi.org/10.1016/B978-0-12-409527-4.00016-X
-
Heo, J.M., Opapeju, F.O., Pluske, J.R., Kim, J.C., Hampson, D.J. and Nyachoti, C.M., 2013. Gastrointestinal health and function in weaned pigs: a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds. Journal of Animal Physiology and Animal Nutrition 97: 207-237. https://doi.org/10.1111/j.1439-0396.2012.01284.x
-
Inoue, R., Tsukahara, T., Nakanishi, N. and Ushida, K., 2005. Development of the intestinal microbiota in the piglet. Journal of General and Applied Microbiology 51: 257-265. https://doi.org/10.2323/jgam.51.257
-
Jensen, J., Kyvsgaard, N.C., Battisti, A. and Baptiste, K.E., 2018. Environmental and public health related risk of veterinary zinc in pig production â using Denmark as an example. Environment International 114: 181-190. https://doi.org/10.1016/j.envint.2018.02.007
-
Kim, H.B. and Isaacson, R.E., 2015. The pig gut microbial diversity: understanding the pig gut microbial ecology through the next generation high throughput sequencing. Veterinary Microbiology 177: 242-251. https://doi.org/10.1016/j.vetmic.2015.03.014
-
Konstantinov, S.R., Awati, A.A., Williams, B.A., Miller, B.G., Jones, P., Stokes, C.R., Akkermans, A.D.L., Smidt, H. and De Vos, W.M., 2006. Post-natal development of the porcine microbiota composition and activities. Environmental Microbiology 8: 1191-1199. https://doi.org/10.1111/j.1462-2920.2006.01009.x
-
LalleÌs, J.-P., Bosi, P., Smidt, H. and Stokes, C.R., 2007. Nutritional management of gut health in pigs around weaning. Proceedings of the Nutrition Society 66: 260-268. https://doi.org/10.1017/S0029665107005484
-
Luppi, A., Gibellini, M., Gin, T., Vangroenweghe, F., Vandenbroucke, V., Bauerfeind, R., Bonilauri, P., Labarque, G. and Hidalgo, AÌ., 2016. Prevalence of virulence factors in enterotoxigenic textitEscherichia coli isolated from pigs with post-weaning diarrhoea in Europe. Porcine Health Management 2: 20. https://doi.org/10.1186/s40813-016-0039-9
-
Mappley, L.J., TchoÌrzewska, M.A., Cooley, W.A., Woodward, M.J. and La Ragione, R.M., 2011. Lactobacilli antagonize the growth, motility, and adherence of Brachyspira pilosicoli: a potential intervention against avian intestinal spirochetosis. Applied and Environmental Microbiology 77: 5402-5411. https://doi.org/10.1128/AEM.00185-11
-
Niu, Q., Li, P., Hao, S., Zhang, Y., Kim, S.W., Li, H., Ma, X., Gao, S., He, L., Wu, W., Huang, X., Hua, J., Zhou, B. and Huang, R., 2015. Dynamic distribution of the gut microbiota and the relationship with apparent crude fiber digestibility and growth stages in pigs. Scientific Reports 5: 9938. https://doi.org/10.1038/srep09938
-
Pluske, J.R., Hampson, D.J. and Williams, I.H., 1997. Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livestock Production Science 51: 215-236. https://doi.org/10.1016/S0301-6226(97)00057-2
-
Pluske, J.R., Turpin, D.L. and Kim, J.-C., 2018. Gastrointestinal tract (gut) health in the young pig. Animal Nutrition 4: 187-196. https://doi.org/10.1016/j.aninu.2017.12.004
-
Rhouma, M., Fairbrother, J.M., Beaudry, F. and Letellier, A., 2017. Post weaning diarrhea in pigs: risk factors and non-colistin-based control strategies. Acta Veterinaria Scandinavica 59: 31. https://doi.org/10.1186/s13028-017-0299-7
-
Sobrino, O.J., Alba, C., Arroyo, R., PeÌrez, I., Sariego, L., Delgado, S., FernaÌndez, L., de MarıÌa, J., Fumanal, P., Fumanal, A. and RodrıÌguez, J.M., 2021. Replacement of metaphylactic antimicrobial therapy by oral administration of Ligilactobacillus salivarius MP100 in a pig farm. Frontiers in Veterinary Science 31: 666887. https:/doi.org/10.3389/fvets.2021.666887
-
Su, Y., Chen, X., Liu, M. and Guo, X., 2017. Effect of three lactobacilli with strain-specific activities on the growth performance, faecal microbiota and ileum mucosa proteomics of piglets. Journal of Animal Science and Biotechnology 8: 52. https://doi.org/10.1186/s40104-017-0183-3
-
Sun, Z., Li, H., Li, Y. and Qiao, J., 2020. Lactobacillus salivarius, a potential probiotic to improve the health of LPS-challenged piglet intestine by alleviating inflammation as well as oxidative stress in a dose-dependent manner during weaning transition. Frontiers in Veterinary Science 7: 547425. https://doi.org/10.3389/fvets.2020.547425
-
Suo, C., Yin, Y., Wang, X., Lou, X., Song, D., Wang, X. and Gu, Q., 2012. Effects of Lactobacillus plantarum ZJ316 on pig growth and pork quality. BMC Veterinary Research 8: 89. https://doi.org/10.1186/1746-6148-8-89
-
Tang, K.L., Caffrey, N.P., NoÌbrega, D.B., Cork, S.C., Ronksley, P.E., Barkema, H.W., Polachek, A.J., Ganshorn, H., Sharma, N., Kellner, J.D. and Ghali, W.A., 2017. Restricting the use of antibiotics in food-producing animals and its associations with antibiotic resistance in food-producing animals and human beings: a systematic review and meta-analysis. Lancet Planet Health 1: e316-e327. https://doi.org/10.1016/S2542-5196(17)30141-9
-
Tao, X., Xu, Z. and Wan, J., 2015. Intestinal microbiota diversity and expression of pattern recognition receptors in newly weaned piglets. Anaerobe 32: 51-56. https://doi.org/10.1016/j.anaerobe.2014.12.005
-
Verstegen, M.W.A. and Williams, B.A., 2002. Alternatives to the use of antibiotics as growth promoters for monogastric animals. Animal Biotechnology 13: 113-127. https://doi.org/10.1081/ABIO-120005774
-
Wang, M., Yang, C., Wang, Q., Li, J., Huang, P., Li, Y., Ding, X., Yang, H. and Yin, Y., 2020a. The relationship between villous height and growth performance, small intestinal mucosal enzymes activities and nutrient transporters expression in weaned piglets. Journal of Animal Physiology and Animal Nutrition 104: 606-615. https://doi.org/10.1111/jpn.13299
-
Wang, M., Yang, C., Wang, Q.Y., Li, J.Z., Li, Y.L., Ding, X.Q., Yin, J., Yang, H.S. and Yin, Y.L., 2020b. The growth performance, intestinal digestive and absorptive capabilities in piglets with different lengths of small intestines. Animal 14: 1196-1203. https://doi.org/10.1017/S175173111900288X
-
Yamazaki, M., Ohtsu, H., Yakabe, Y., Kishima, M. and Abe, H., 2012. In vitro screening of lactobacilli isolated from chicken excreta to control Salmonella Enteritidis and Typhimurium. British Poultry Science 53: 183-189. https://doi.org/10.1080/00071668.2012.678814
-
Zeng, M.Y., Inohara, N. and NunÌez, G., 2017. Mechanisms of inflammation-driven bacterial dysbiosis in the gut. Mucosal Immunology 10: 18-26. https://doi.org/10.1038/mi.2016.75
-
Zhang, J., Deng, J., Wang, Z., Che, C., Li, Y. and Yang, Q., 2011. Modulatory effects of Lactobacillus salivarius on intestinal mucosal immunity of piglets. Current Microbiology 62: 1623-1631. https://doi.org/10.1007/s00284-011-9906-4
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 1250 | 196 | 17 |
| å ¨ææµè§æ¬¡æ° | 45 | 13 | 0 |
| PDFä¸è½½æ¬¡æ° | 78 | 29 | 0 |
Feed supplementation with Ligilactobacillus salivarius PS21603 optimises intestinal morphology and gut microbiota composition in weaned piglets
äºBeneficial MicrobesSearch for other papers by I. Cuevas-GoÌmez in
Current site
Google Scholar
PubMed
Search for other papers by J. de AndreÌs in
Current site
Google Scholar
PubMed
Search for other papers by N. Cardenas in
Current site
Google Scholar
PubMed
Search for other papers by I. Espinosa-Martos in
Current site
Google Scholar
PubMed
Search for other papers by E. JimeÌnez in
Current site
Google Scholar
PubMed
Abstract
Post-weaning diarrhoea in piglets remains an important cause of economic losses for swine producers. Feed supplementation with probiotics is one of the alternatives to antibiotics used to reduce the impact of such gastrointestinal disease. The aim of the present study was to evaluate the effect of Ligilactobacillus salivarius PS21603 supplementation on the intestinal structure and the gut microbiota composition of weaned piglets. Safety and tolerance of L. salivarius PS21603 were previously evaluated in a 28-days study using 384 weaned piglets (28 ± 2 days old and 7.5 ± 1.5 kg) divided in three treatment groups: T1: Basal diet + L. salivarius PS21603 109 cfu/day, T2: Basal diet + L. salivarius PS21603 107 cfu/day, and T3: Basal diet (control group). For the present study, 16 piglets per treatment group were randomly selected and faecal samples were collected on day 0 (weaning) and 28 of study. At the end of study, three males and three females per treatment were euthanised. Intestinal morphometric values were measured after necropsy. Faecal counts of Escherichia coli were evaluated by culture techniques, and faecal microbiota composition was assessed by high-throughput sequencing. All data were analysed and compared between treatment groups. Supplementation with L. salivarius PS21603 caused an increase in the intestine length of piglets from T1 and in the villous height:crypt ratio of piglets from T2 (P < 0.05) compared to T3 on day 28. According to the Shannon Diversity Index, microbiota diversity increased on day 28 compared to day 0, with no significant differences observed between treatments. The main changes in the relative abundance of bacteria at the phylum, family, and genus levels were observed between different sampling time points. However, piglets from T1 and T2 had lower faecal E. coli counts than T3 on day 28 (P < 0.05). Moreover, supplementation with L. salivarius PS21603 modulated gut microbiota through a more optimal composition, reducing Escherichia and increasing Bifidobacterium relative abundance in piglets from T1 (P < 0.05) from the beginning to the end of the study. Therefore, the strain L. salivarius PS21603 has shown probiotic properties to be used as feed additive in the pig industry, along with good hygiene and farm management practices, for the prevention and/or treatment of post-weaning diarrhoea in piglets.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 1250 | 196 | 17 |
| å ¨ææµè§æ¬¡æ° | 45 | 13 | 0 |
| PDFä¸è½½æ¬¡æ° | 78 | 29 | 0 |
