Safety assessment of Streptococcus salivarius M18 a probiotic for oral health
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The development of probiotics targeting non-intestinal body sites continues to generate interest amongst researchers, biotech companies and consumers alike. A key consideration for any bacterial strain to be developed into a probiotic is a robust assessment of its safety profile. Streptococcus salivarius strain M18 was originally isolated from a healthy adult and evaluated for its probiotic capabilities targeted to dental and oral health applications. This publication presents the safety characterisation of strain M18. Application of a diverse range of techniques showed that strain M18 can be specifically distinguished from other S. salivarius using a variety of molecular and phenotypic methodologies and that it lacks any relevant antibiotic resistance or virulence determinants. Direct comparison of the strain M18 safety profile with that of the prototype S. salivarius probiotic, S. salivarius strain K12, supports the proposition that strain M18 is indeed safe for probiotic application in humans.
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-
Appelbaum, P.C., 2002. Resistance among Streptococcus pneumoniae: implications for drug selection. Clinical Infectious Diseases 34: 1613-1620. https://doi.org/10.1086/340400
-
Bardellini, E., Amadori, F., Gobbi, E., Ferri, A., Conti, G. and Majorana, A., 2020. Does Streptococcus salivarius strain M18 assumption make black stains disappear in children? Oral Health and Preventive Dentistry 18: 161-164. https://doi.org/10.3290/j.ohpd.a43359
-
Barretto, C., Alvarez-Martin, P., Foata, F., Renault, P. and Berger, B., 2012. Genome sequence of the lantibiotic bacteriocin producer Streptococcus salivarius strain K12. Journal of Bacteriology 194: 5959-5960. https://doi.org/10.1128/JB.01268-12
-
Benic, G.Z., Farella, M., Morgan, X.C., Viswam, J., Heng, N.C., Cannon, R.D. and Mei, L., 2019. Oral probiotics reduce halitosis in patients wearing orthodontic braces: a randomized, triple-blind, placebocontrolled trial. Journal of Breath Research 13: 36010. https://doi.org/10.1088/1752-7163/AB1C81
-
Bolotin, A., Quinquis, B., Renault, P., Sorokin, A., Ehrlich, S.D., Kulakauskas, S., Lapidus, A., Goltsman, E., Mazur, M., Pusch, G.D., Fonstein, M., Overbeek, R., Kyprides, N., Purnelle, B., Prozzi, D., Ngui, K., Masuy, D., Hancy, F., Burteau, S., Boutry, M., Delcour, J., Goffeau, A. and Hols, P., 2004. Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus. Nature Biotechnology 22: 1554-1558. https://doi.org/10.1038/nbt1034
-
Borges, S., Silva, J. and Teixeira, P., 2014. The role of lactobacilli and probiotics in maintaining vaginal health. Archives of Gynecology and Obstetrics 289: 479-489. https://doi.org/10.1007/s00404-013-3064-9
-
Borriello, S.P., Hammes, W.P., Holzapfel, W., Marteau, P., Schrezenmeir, J., Vaara, M. and Valtonen, V., 2003. Safety of probiotics that contain lactobacilli or bifidobacteria. Clinical Infectious Disease 36: 775-780. https://doi.org/10.1086/368080
-
Bourdichon, F., Casaregola, S., Farrokh, C., Frisvad, J.C., Gerds, M.L., Hammes, W.P., Harnett, J., Huys, G., Laulund, S., Ouwehand, A., Powell, I.B., Prajapati, J.B., Seto, Y., Ter Schure, E., Van Boven, A., Vankerckhoven, V., Zgoda, A., Tuijtelaars, S. and Hansen, E.B., 2012. Food fermentations: microorganisms with technological beneficial use. International Journal of Food Microbiology 154: 87-97. https://doi.org/10.1016/j.ijfoodmicro.2011.12.030
-
Burton, J. P., Chilcott, C.N., Moore, C.J., Speiser, G. and Tagg, J.R., 2006a. A preliminary study of the effect of probiotic Streptococcus salivarius K12 on oral malodour parameters. Journal of Applied Microbiology 100: 754-764. https://doi.org/10.1111/j.1365-2672.2006.02837.x
-
Burton, J.P., Chilcott, C.N., Wescombe, P.A. and Tagg, J.R., 2010. Extended safety data for the oral cavity probiotic Streptococcus salivarius K12. Probiotics and Antimicrobial Proteins 2: 135-144. https://doi.org/10.1007/s12602-010-9045-4
-
Burton, J.P., Cowley, S., Simon, R.R., McKinney, J., Wescombe, P.A. and Tagg, J.R., 2011. Evaluation of safety and human tolerance of the oral probiotic Streptococcus salivarius K12: a randomized, placebocontrolled, double-blind study. Food and Chemical Toxicology 49: 2356-2364. https://doi.org/10.1016/j.fct.2011.06.038
-
Burton, J.P., Drummond, B.K., Chilcott, C.N., Tagg, J.R., Thomson, W.M., Hale, J.D.F. and Wescombe, P.A., 2013a. Influence of the probiotic Streptococcus salivarius strain M18 on indices of dental health in children: a randomized double-blind, placebo-controlled trial. Journal of Medical Microbiology 62: 875-884. https://doi.org/10.1099/jmm.0.056663-0
-
Burton, J.P., Wescombe, P.A., Macklaim, J.M., Chai, M.H.C., MacDonald, K., Hale, J.D.F., Tagg, J., Reid, G., Gloor, G.B. and Cadieux, P.A., 2013b. Persistence of the oral probiotic Streptococcus salivarius M18 is dose dependent and megaplasmid transfer can augment their bacteriocin production and adhesion characteristics. PLoS ONE 13: e65991. https://doi.org/10.1371/journal.pone.0065991
-
Burton, J.P., Wescombe, P.A., Moore, C.J., Chilcott, C.N. and Tagg, J.R., 2006b. Safety assessment of the oral cavity probiotic. Applied and Environmental Microbiology 72: 2050-2053. https://doi.org/10.1128/AEM.72.4.3050
-
Chilcott, C.N. and Tagg, J.R., 2007. Antimicrobial composition. US Patent 7226590. Available at: https://patents.google.com/patent/US7226590B2/en
-
Cosentino, S., Voldby Larsen, M., Møller Aarestrup, F. and Lund, O., 2013a. PathogenFinder – distinguishing friend from foe using bacterial whole genome sequence data. PLoS ONE 8: e77302. https://doi.org/10.1371/journal.pone.0077302
-
Delorme, C., Abraham, A.L., Renault, P. and Guédon, E., 2015. Genomics of Streptococcus salivarius, a major human commensal. Infection, Genetics and Evolution 33: 381-392. https://doi.org/10.1016/j.meegid.2014.10.001
-
Delorme, C., Poyart, C., Ehrlich, S.D. and Renault, P., 2007. Extent of horizontal gene transfer in evolution of streptococci of the salivarius group. Journal of Bacteriology 189: 1330-1341. https://doi.org/10.1128/JB.01058-06
-
Di Pierro, F., Colombo, M., Zanvit, A. and Rottoli, A.S., 2016. Positive clinical outcomes derived from using Streptococcus salivarius K12 to prevent streptococcal pharyngotonsillitis in children: a pilot investigation. Drug, Healthcare and Patient Safety 8: 77-81. https://doi.org/10.2147/DHPS.S117214
-
Di Pierro, F., Colombo, M., Zanvit, A., Risso, P. and Rottoli, A.S., 2014. Use of Streptococcus salivarius K12 in the prevention of streptococcal and viral pharyngotonsillitis in children. Drug, Healthcare and Patient Safety 6: 15-20. https://doi.org/10.2147/dhps.s59665
-
Di Pierro, F., Donato, G., Fomia, F., Adami, T., Careddu, D., Cassandro, C. and Albera, R., 2012. Preliminary pediatric clinical evaluation of the oral probiotic Streptococcus salivarius K12 in preventing recurrent pharyngitis and/or tonsillitis caused by Streptococcus pyogenes and recurrent acute otitis media. International Journal of General Medicine 5: 991-997. https://doi.org/10.2147/IJGM.S38859
-
Di Pierro, F., Zanvit, A., Nobili, P., Risso, P. and Fornaini, C., 2015. Cariogram outcome after 90 days of oral treatment with Streptococcus salivarius M18 in children at high risk for dental caries: results of a randomized, controlled study. Clinical, Cosmetic and Investigational Dentistry 7: 107-113. https://doi.org/10.2147/CCIDE.S93066
-
European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID), 2003. Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution. Clinical Microbiology and Infection 9: 9-15. https://doi.org/10.1046/j.1469-0691.2003.00790.x
-
European Food Safety Authority (EFSA), 2012. Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA Journal 10: 2740. https://doi.org/10.2903/j.efsa.2012.2740
-
Falagas, M.E., Betsi, G.I. and Athanasiou, S., 2007. Probiotics for the treatment of women with bacterial vaginosis. Clinical Microbiology and Infection 9: 657-664. https://doi.org/10.1111/j.1469-0691.2007.01688.x
-
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2001. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Available at: http://tinyurl.com/8bccc3r.
-
Forsberg, K.J., Patel, S., Gibson, M.K., Lauber, C.L., Knight, R., Fierer, N. and Dantas, G., 2014. Bacterial phylogeny structures soil resistomes across habitats. Nature 509: 612-616. https://doi.org/10.1038/nature13377
-
Fu, L., Niu, B., Zhu, Z., Wu, S. and Li, W., 2012. CD-HIT: accelerated for clustering the next-generation sequencing data. Bioinformatics 28: 3150-3152. https://doi.org/10.1093/bioinformatics/bts565
-
Gibbons, R.J. and Houte, J.V., 1975. Bacterial adherence in oral microbial ecology. Annual Review of Microbiology 29: 19-44. https://doi.org/10.1146/annurev.mi.29.100175.000315
-
Guglielmetti, S., Taverniti, V., Minuzzo, M., Arioli, S., Stuknyte, M., Karp, M. and Mora, D., 2010. Oral bacteria as potential probiotics for the pharyngeal mucosa. Applied and Environmental Microbiology 76: 3948-3958. https://doi.org/10.1128/AEM.00109-10
-
Hasman, H., Saputra, D., Sicheritz-Ponten, T., Lund, O., Svendsen, C.A., Frimodt-Moller, N. and Aarestrup, F.M., 2014. Rapid whole-genome sequencing for detection and characterization of microorganisms directly from clinical samples. Journal of Clinical Microbiology 52L: 139-146. https://doi.org/10.1128/JCM.02452-13
-
Hegde, S. and Munshi, A.K., 1998. Influence of the maternal vaginal microbiota on the oral microbiota of the newborn. Journal of Clinical Pediatric Dentistry 22: 317-321.
'Influence of the maternal vaginal microbiota on the oral microbiota of the newborn ' () 22 Journal of Clinical Pediatric Dentistry : 317 -321.
-
Heng, N.C.K., Haji-Ishak, N.S., Kalyan, A., Wong, A.Y.C., Lovric, M., Bridson, J.M., Artamonova, J., Stanton, J.A.L., Wescombe, P.A., Burton, J.P., Cullinan, M.P. and Tagg, J.R., 2011. Genome sequence of the bacteriocin-producing oral probiotic Streptococcus salivarius strain M18. Journal of Bacteriology 193: 6402-6403. https://doi.org/10.1128/JB.06001-11
-
Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein, D.J., Pot, B., Morelli, L., Canani, R.B., Flint, H.J., Salminen, S., Calder, P.C. and Sanders, M.E., 2014. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics Consensus Statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology & Hepatology 11: 506-514. https://doi.org/10.1038/nrgastro.2014.66
-
Horz, H.P., Meinelt, A., Houben, B. and Conrads, G., 2007. Distribution and persistence of probiotic Streptococcus salivarius K12 in the human oral cavity as determined by real-time quantitative polymerase chain reaction. Oral Microbiology and Immunology 22: 126-130. https://doi.org/10.1111/j.1399-302X.2007.00334.x
-
Hyink, O., Wescombe, P.A., Upton, M., Ragland, N., Burton, J.P. and Tagg, J.R., 2007. Salivaricin A2 and the novel lantibiotic salivaricin B are encoded at adjacent loci on a 190-kilobase transmissible megaplasmid in the oral probiotic strain Streptococcus salivanus K12. Applied and Environmental Microbiology 73: 1107-1113. https://doi.org/10.1128/AEM.02265-06
-
Jamali, Z., Aminabadi, N.A., Samiei, M., Deljavan, A.S., Shokravi, M. and Shirazi, S., 2016. Impact of chlorhexidine pretreatment followed by probiotic Streptococcus salivarius strain K12 on halitosis in children: a randomised controlled clinical trial. Oral Health & Preventive Dentistry 14: 305-313. https://doi.org/10.3290/j.ohpd.a36521
-
Kaci, G., Goudercourt, D., Dennin, V., Pot, B., Doré, J., Ehrlich, S.D., Renault, P., Blottière, H.M., Daniel, C. and Delorme, C., 2014. Antiinflammatory properties of Streptococcus salivarius, a commensal bacterium of the oral cavity and digestive tract. Applied and Environmental Microbiology 80: 928-934. https://doi.org/10.1128/AEM.03133-13
-
Kober, M.M. and Bowe, W.P., 2015. The effect of probiotics on immune regulation, acne, and photoaging. International Journal of Women’s Dermatology 6: 85-89. https://doi.org/10.1016/j.ijwd.2015.02.001
-
Larsen, M.V., Cosentino, S., Rasmussen, S., Friis, C., Hasman, H., Marvig, R.L., Jelsbak, L., Sicheritz-Pontén, T., Ussery, D.W., Aarestrup, F.M. and Lund, O., 2012. Multilocus sequence typing of total-genome-sequenced bacteria. Journal of Clinical Microbiology 50: 1355-1361. https://doi.org/10.1128/JCM.06094-11
-
Lew, L.C. and Liong, M.T., 2013. Bioactives from probiotics for dermal health: functions and benefits. Journal of Applied Microbiology 114: 1241-1253. https://doi.org/10.1111/jam.12137
-
Macdonald, K.W., Chanyi, R.M., Macklaim, J.M., Cadieux, P.A., Reid, G. and Burton, J.P., 2020. Deep learning-based dental plaque detection on primary teeth: a comparison with clinical assessments. BMC Oral Health 21: 245. https://doi.org/10.1186/s12903-021-01606-z
-
Mackowiak, P.A., 2013. Recycling Metchnikoff: probiotics, the intestinal microbiome and the quest for long life. Frontiers in Public Health 1: 52. https://doi.org/10.3389/fpubh.2013.00052
-
Martín, V., Mediano, P., Del Campo, R., Rodríguez, J.M. and Marín, M., 2016. Streptococcal diversity of human milk and comparison of different methods for the taxonomic identification of streptococci. Journal of Human Lactation 32: NP84-NP94. https://doi.org/10.1177/0890334415597901
-
Matsumoto, M., Suzuki, Y., Miyazaki, Y., Tanaka, D., Yasuoka, T., Mashiko, K., Ishikita, R. and Baba, J., 2001. Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR); its ability to differentiate Streptococcus pyogenes strains and applicability to the study of outbreaks of streptococcal infection. Tohoku Journal of Experimental Medicine 194: 205-212. https://doi.org/10.1620/tjem.194.205
-
Mogensen, G., Salminen, S., O’Brien, J., Ouwehand, A., Holzapfel, W., Shortt, C., Fonden, R., Miller, G.D., Donohue, D., Playne, M., Crittenden, R., Salvadori, B.B. and Zink, R., 2002. Inventory of microorganisms with a documented history of use in food. Bulletin of the International Dairy Federation 377: 10-19.
'Inventory of microorganisms with a documented history of use in food ' () 377 Bulletin of the International Dairy Federation : 10 -19.
-
Moore, A.M., Patel, S., Forsberg, K.J., Wang, B., Bentley, G., Razia, Y., Qin, X., Tarr, P.I. and Dantas, G., 2013. Pediatric fecal microbiota harbor diverse and novel antibiotic resistance genes. PLoS ONE 8: e78822. https://doi.org/10.1371/journal.pone.0078822
-
Pehrsson, E.C., Tsukayama, P., Patel, S., Mejía-Bautista, M., Sosa-Soto, G., Navarrete, K.M., Calderon, M., Cabrera, L., Hoyos-Arango, W., Bertoli, M.T., Berg, D.E., Gilman, R.H. and Dantas, G., 2016. Interconnected microbiomes and resistomes in low-income human habitats. Nature 533: 212-216. https://doi.org/10.1038/nature17672
-
Porter, S.R., Scully, C. and Hegarty, A.M., 2004. An update of the etiology and management of xerostomia. Oral Surgery, Oral Medicine, Oral Pathology Oral Radiology 97: 28-46. https://doi.org/10.1016/j.tripleo.2003.07.010
-
Scariya, L., Nagarathna, D.V. and Varghese, M., 2015. Probiotics in periodontal therapy. International Journal of Pharma and Bio Sciences 6: 242-250. https://doi.org/10.5455/musbed.20141106034910
-
Seemann, T., 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30: 2068-2069. https://doi.org/10.1093/bioinformatics/btu153
-
Sommer, M.O.A., Dantas, G. and Church, G.M., 2009. Functional characterization of the antibiotic resistance reservoir in the human microflora. Science 325: 1128-1131. https://doi.org/10.1126/science.1176950
-
Tagg, J.R. and Bannister, L.V., 1979. ‘Fingerprinting’ β-haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors. Journal of Medical Microbiology 12: 397-411. https://doi.org/10.1099/00222615-12-4-397
-
Tandelilin, R.C., Widita, E., Agustina, D. and Saini, R., 2018. The effect of oral probiotic consumption on the caries risk factors among high-risk caries population. Journal of International Oral Health 10: 132. https://doi.org/10.4103/jioh.jioh_82_18
-
Tompkins, G.R. and Tagg, J.R., 1989. The ecology of bacteriocin-producing strains of Streptococcus salivarias. Microbial Ecology in Health and Disease 2: 19-28. https://doi.org/10.3109/08910608909140197
-
United States Food and Drug Administration (US FDA), 2016. Agency response letter GRAS notice No. GRN 000591. US FDA, Silver Spring, MD, USA.
-
United States Food and Drug Administration (US FDA), 2018. Agency response letter GRAS notice No. GRN 000807. US FDA, Silver Spring, MD, USA.
-
Watanabe, S., Ohnishi, M., Imai, K., Kawano, E. and Igarashi, S., 1995. Estimation of the total saliva volume produced per day in five-year-old children. Archives of Oral Biology 40: 781-782. https://doi.org/10.1016/0003-9969(95)00026-L
-
Wescombe, P.A., Burton, J.P., Cadieux, P.A., Klesse, N.A., Hyink, O., Heng, N.C.K., Chilcott, C.N., Reid, G. and Tagg, J.R., 2006a. Megaplasmids encode differing combinations of lantibiotics in Streptococcus salivarias. Antonie van Leeuwenhoek 90: 269-280. https://doi.org/10.1007/s10482-006-9081-y
-
Wescombe, P.A., Hale, J.D.F., Heng, N.C.K. and Tagg, J.R., 2012. Developing oral probiotics from Streptococcus salivarias. Future Microbiology 7: 1355-1371. https://doi.org/10.2217/fmb.12.113
-
Wescombe, P.A., Heng, N.C.K., Burton, J.P. and Tagg, J.R., 2010. Something old and something new: an update on the amazing repertoire of bacteriocins produced by Streptococcus salivarias. Probiotics and Antimicrobial Proteins 2: 37-45. https://doi.org/10.1007/s12602-009-9026-7
-
Wescombe, P.A., Heng, N.C.K., Burton, J.P., Chilcott, C.N. and Tagg, J.R., 2009. Streptococcal bacteriocins and the case for Streptococcus salivarius as model oral probiotics. Future Microbiology 4: 819-835. https://doi.org/10.2217/fmb.09.61
-
Wescombe, P.A., Upton, M., Dierksen, K.P., Ragland, N.L., Sivabalan, S., Wirawan, R.E., Inglis, M.A., Moore, C.J., Walker, G.V, Chilcott, C.N., Jenkinson, H.F. and Tagg, J.R., 2006b. Production of the lantibiotic salivaricin A and its variants by oral streptococci and use of a specific induction assay to detect their presence in human saliva. Applied and Environmental Microbiology 72: 1459-1466. https://doi.org/10.1128/AEM.72.2.1459-1466.2006
-
Wescombe, P.A., Upton, M., Renault, P., Wirawan, R.E., Power, D., Burton, J.P., Chilcott, C.N. and Tagg, J.R., 2011. Salivaricin 9, a new lantibiotic produced by Streptococcus salivarius. Microbiology 157: 1290-1299. https://doi.org/10.1099/mic.0.044719-0
-
Zankari, E., Hasman, H., Cosentino, S., Vestergaard, M., Rasmussen, S., Lund, O., Aarestrup, F.M. and Larsen, M.V., 2012. Identification of acquired antimicrobial resistance genes. Journal of Antimicrobial Chemotherapy 67: 2640-2644. https://doi.org/10.1093/jac/dks261
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Safety assessment of Streptococcus salivarius M18 a probiotic for oral health
In: Beneficial MicrobesSearch for other papers by J.D.F. Hale in
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Search for other papers by R. Jain in
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Search for other papers by J.P. Burton in
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PubMed
The development of probiotics targeting non-intestinal body sites continues to generate interest amongst researchers, biotech companies and consumers alike. A key consideration for any bacterial strain to be developed into a probiotic is a robust assessment of its safety profile. Streptococcus salivarius strain M18 was originally isolated from a healthy adult and evaluated for its probiotic capabilities targeted to dental and oral health applications. This publication presents the safety characterisation of strain M18. Application of a diverse range of techniques showed that strain M18 can be specifically distinguished from other S. salivarius using a variety of molecular and phenotypic methodologies and that it lacks any relevant antibiotic resistance or virulence determinants. Direct comparison of the strain M18 safety profile with that of the prototype S. salivarius probiotic, S. salivarius strain K12, supports the proposition that strain M18 is indeed safe for probiotic application in humans.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1575 | 477 | 33 |
| Full Text Views | 92 | 16 | 0 |
| PDF Views & Downloads | 178 | 34 | 0 |
