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Gut microbial differences in breast and prostate cancer cases from two randomised controlled trials compared to matched cancer-free controls
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Implicated in several chronic diseases, the gastrointestinal microbiome is hypothesised to influence carcinogenesis. We compared faecal microbiota of newly diagnosed treatment-naïve overweight and obese cancer patients and matched controls. Cases were enrolled in presurgical weight-loss trials for breast (NCT02224807) and prostate (NCT01886677) cancers and had a body mass index (BMI) ≥25 kg/m2. Cancer-free controls were matched 1:1 by age (±5 years), race, gender, and BMI (±5 kg/m2). All participants provided faecal samples; isolated bacterial DNA were PCR amplified at the V4 region of the 16S rRNA gene and analysed using the QIIME pipeline. Tests compared cases versus controls, then separately by gender. Microbial alpha-diversity and beta-diversity were assessed, and relative abundance of Operational Taxonomic Units (OTU’s) were compared at the genus level, with false discovery rate (FDR) correction. 22 overweight and obese cancer patients were matched with 22 cancer-free controls, with an average BMI of 30.5±4.3 kg/m2, age 54.4±5.3 years, and 54.5% were black. Fourteen matches were made between breast cancer cases and healthy female controls, and 8 matches were made with prostate cancer cases and healthy male controls. Comparison of all cases and controls revealed no differences in alpha diversity, though prostate cancer patients had higher Chao1 (P=0.006) and Observed Species (P=0.036) than cancer-free males. Beta-diversity metrics were significantly different between cases and controls (P<0.03 for all tests in whole sample and in men), though only unweighted Unifrac was different in women (P=0.005). Kruskal Wallis tests indicated significant differences among 16 genera in all matches, 9 in female, and 51 in male. This study suggests the faecal microbiota of treatment-naive breast and prostate cancer patients differs from controls, though larger samples are needed to substantiate these findings. Trial registration: NIH Clinical Trials, NCT01886677, NCT02224807, registered 26 June 2013, 25 Aug 2014 (respectively) – retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT01886677; https://clinicaltrials.gov/ct2/show/NCT02224807
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Attene-Ramos, M.S., Wagner, E.D., Gaskins, H.R. and Plewa, M.J., 2007. Hydrogen sulfide induces direct radical-associated DNA damage. Molecular Cancer Research 5: 455. https://doi.org/10.1158/1541-7786.MCR-06-0439
-
Benson, A.K., Kelly, S.A., Legge, R., Ma, F., Low, S.J., Kim, J., Zhang, M., Oh, P.L., Nehrenberg, D., Hua, K., Kachman, S.D., Moriyama, E.N., Walter, J., Peterson, D.A. and Pomp, D., 2010. Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. Proceedings of the National Academy of Sciences of the USA 107: 18933. https://doi.org/10.1073/pnas.1007028107
-
Bouter, K.E., van Raalte, D.H., Groen, A.K. and Nieuwdorp, M., 2017. Role of the gut microbiome in the pathogenesis of obesity and obesity-related metabolic dysfunction. Gastroenterology 152: 1671-1678. https://doi.org/10.1053/j.gastro.2016.12.048
-
Bultman, S.J., 2014. Emerging roles of the microbiome in cancer. Carcinogenesis 35: 249-255. https://doi.org/10.1093/carcin/bgt392
-
Bultman, S.J., 2016. The microbiome and its potential as a cancer preventive intervention. Seminars in Oncology 43: 97-106. https://doi.org/10.1053/j.seminoncol.2015.09.001
-
Camilleri, M., Madsen, K., Spiller, R., Van Meerveld, B.G. and Verne, G.N., 2012. Intestinal barrier function in health and gastrointestinal disease. Neurogastroenterology and Motility 24: 503-512. https://doi.org/10.1111/j.1365-2982.2012.01921.x
-
Cani, P.D., Amar, J., Iglesias, M.A., Poggi, M., Knauf, C., Bastelica, D., Neyrinck, A.M., Fava, F., Tuohy, K.M., Chabo, C., Waget, A., Delmée, E., Cousin, B., Sulpice, T., Chamontin, B., Ferrières, J., Tanti, J.F., Gibson, G.R., Casteilla, L., Delzenne, N.M., Alessi, M.C. and Burcelin, R., 2007. Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56: 1761-1772.
'Metabolic endotoxemia initiates obesity and insulin resistance ' () 56 Diabetes : 1761 -1772.
-
Carson, T.L., Wang, F., Cui, X., Jackson, B.E., Van Der Pol, W.J., Lefkowitz, E.J., Morrow, C. and Baskin, M.L., 2018. Associations between race, perceived psychological stress, and the gut microbiota in a sample of generally healthy black and white women. Psychosomatic Medicine 80: 640-648. https://doi.org/10.1097/psy.0000000000000614
-
Daft, J.G., Ptacek, T., Kumar, R., Morrow, C. and Lorenz, R.G., 2015. Cross-fostering immediately after birth induces a permanent microbiota shift that is shaped by the nursing mother. Microbiome 3: 17. https://doi.org/10.1186/s40168-015-0080-y
-
Demark-Wahnefried, W., Nix, J.W., Hunter, G.R., Rais-Bahrami, S., Desmond, R.A., Chacko, B., Morrow, C.D., Azrad, M., Frugé, A.D., Tsuruta, Y., Ptacek, T., Tully, S.A., Segal, R. and Grizzle, W.E., 2016. Feasibility outcomes of a presurgical randomized controlled trial exploring the impact of caloric restriction and increased physical activity versus a wait-list control on tumor characteristics and circulating biomarkers in men electing prostatectomy for prostate cancer. BMC Cancer 16: 61. https://doi.org/10.1186/s12885-016-2075-x
-
Demark-Wahnefried, W., Rais-Bahrami, S., Desmond, R.A., Gordetsky, J.B., Hunter, G.R., Yang, E.S., Azrad, M., Frugé, A.D., Tsuruta, Y., Norian, L.A., Segal, R. and Grizzle, W.E., 2017. Presurgical weight loss affects tumour traits and circulating biomarkers in men with prostate cancer. British Journal of Cancer 117: 1303-1313. https://doi.org/10.1038/bjc.2017.303
-
Dominianni, C., Sinha, R., Goedert, J.J., Pei, Z., Yang, L., Hayes, R.B. and Ahn, J., 2015. Sex, body mass index, and dietary fiber intake influence the human gut microbiome. PLoS ONE 10: e0124599. https://doi.org/10.1371/journal.pone.0124599
-
Fernández, M., Reina-Pérez, I., Astorga, J., Rodríguez-Carrillo, A., Plaza-Díaz, J. and Fontana, L., 2018. Breast cancer and its relationship with the microbiota. International Journal of Environmental Research and Public Health 15: 1747. https://doi.org/10.3390/ijerph15081747
-
Flint, H.J., Scott, K.P., Louis, P. and Duncan, S.H., 2012. The role of the gut microbiota in nutrition and health. Nature Reviews Gastroenterology and Hepatology 9: 577. https://doi.org/10.1038/nrgastro.2012.156
-
Frugé, A.D., Ptacek, T., Tsuruta, Y., Morrow, C.D., Azrad, M., Desmond, R.A., Hunter, G.R., Rais-Bahrami, S. and Demark-Wahnefried, W., 2018. Dietary changes impact the gut microbe composition in overweight and obese men with prostate cancer undergoing radical prostatectomy. Journal of the Academy of Nutrition and Dietetics 118: 714-723.e1. https://doi.org/10.1016/j.jand.2016.10.017
-
Frugé, A.D., Van der Pol, W., Rogers, L.Q., Morrow, C.D., Tsuruta, Y. and Demark-Wahnefried, W., 2020. Fecal Akkermansia muciniphila is associated with body composition and microbiota diversity in overweight and obese women with breast cancer participating in a presurgical weight loss trial. Journal of the Academy of Nutrition and Dietetics 120: 650-659. https://doi.org/10.1016/j.jand.2018.08.164
-
Goedert, J.J., Jones, G., Hua, X., Xu, X., Yu, G., Flores, R., Falk, R.T., Gail, M.H., Shi, J., Ravel, J. and Feigelson, H.S., 2015. Investigation of the association between the fecal microbiota and breast cancer in postmenopausal women: a population-based case-control pilot study. Journal of the National Cancer Institute 107: 147. https://doi.org/10.1093/jnci/djv147
-
Golombos, D.M., Ayangbesan, A., O’Malley, P., Lewicki, P., Barlow, L.M., Barbieri, C.E., Chan, C., DuLong, C., Abu-Ali, G., Huttenhower, C. and Scherr, D.S., 2018. The role of gut microbiome in the pathogenesis of prostate cancer: a prospective, pilot study. Urology 111: 122-128. https://doi.org/10.1016/j.urology.2017.08.039
-
Goodrich, J.K., Waters, J.L., Poole, A.C., Sutter, J.L., Koren, O., Blekhman, R., Beaumont, M., Van Treuren, W., Knight, R., Bell, J.T., Spector, T.D., Clark, A.G. and Ley, R.E., 2014. Human genetics shape the gut microbiome. Cell 159: 789-799. https://doi.org/10.1016/j.cell.2014.09.053
-
Grivennikov, S.I., Greten, F.R. and Karin, M., 2010. Immunity, inflammation, and cancer. Cell 140: 883-899. https://doi.org/10.1016/j.cell.2010.01.025
-
Howe, L.R., Subbaramaiah, K., Hudis, C.A. and Dannenberg, A.J., 2013. Molecular pathways: adipose inflammation as a mediator of obesity-associated cancer. Clinical Cancer Research 19: 6074. https://doi.org/10.1158/1078-0432.CCR-12-2603
-
Hsieh, Y.Y., Tung, S.Y., Pan, H.Y., Yen, C.W., Xu, H.W., Lin, Y.J., Deng, Y.F., Hsu, W.T., Wu, C.S. and Li, C., 2018. Increased Abundance of Clostridium and Fusobacterium in Gastric Microbiota of Patients with Gastric Cancer in Taiwan. Scientific Reports 8: 158. https://doi.org/10.1038/s41598-017-18596-0
-
Huttenhower, C., Gevers, D., Knight, R., Abubucker, S., Badger, J.H., Chinwalla, A.T., Creasy, H.H., Earl, A.M., Fitzgerald, M.G., Fulton, R.S., Giglio, M.G., Hallsworth-Pepin, K., Lobos, E.A., Madupu, R., Magrini, V., Martin, J.C., Mitreva, M., Muzny, D.M., Sodergren, E.J., Versalovic, J., Wollam, A.M., Worley, K.C., Wortman, J.R., Young, S.K., Zeng, Q., Aagaard, K.M., Abolude, O.O., Allen-Vercoe, E., Alm, E.J., Alvarado, L., Andersen, G.L., Anderson, S., Appelbaum, E., Arachchi, H.M., Armitage, G., Arze, C.A., Ayvaz, T., Baker, C.C., Begg, L., Belachew, T., Bhonagiri, V., Bihan, M., Blaser, M.J., Bloom, T., Bonazzi, V., Paul Brooks, J., Buck, G.A., Buhay, C.J., Busam, D.A., Campbell, J.L., Canon, S.R., Cantarel, B.L., Chain, P.S.G., Chen, I.M.A., Chen, L., Chhibba, S., Chu, K., Ciulla, D.M., Clemente, J.C., Clifton, S.W., Conlan, S., Crabtree, J., Cutting, M.A., Davidovics, N.J., Davis, C.C., Desantis, T.Z., Deal, C., Delehaunty, K.D., Dewhirst, F.E., Deych, E., Ding, Y., Dooling, D.J., Dugan, S.P., Michael Dunne, W., Scott Durkin, A., Edgar, R.C., Erlich, R.L., Farmer, C.N., Farrell, R.M., Faust, K., Feldgarden, M., Felix, V.M., Fisher, S., Fodor, A.A., Forney, L.J., Foster, L., Di Francesco, V., Friedman, J., Friedrich, D.C., Fronick, C.C., Fulton, L.L., Gao, H., Garcia, N., Giannoukos, G., Giblin, C., Giovanni, M.Y., Goldberg, J.M., Goll, J., Gonzalez, A., Griggs, A., Gujja, S., Kinder Haake, S., Haas, B.J., Hamilton, H.A., Harris, E.L., Hepburn, T.A., Herter, B., Hoffmann, D.E., Holder, M.E., Howarth, C., Huang, K.H., Huse, S.M., Izard, J., Jansson, J.K., Jiang, H., Jordan, C., Joshi, V., Katancik, J.A., Keitel, W.A., Kelley, S.T., Kells, C., King, N.B., Knights, D., Kong, H.H., Koren, O., Koren, S., Kota, K.C., Kovar, C.L., Kyrpides, N.C., La Rosa, P.S., Lee, S.L., Lemon, K.P., Lennon, N., Lewis, C.M., Lewis, L., Ley, R.E., Li, K., Liolios, K., Liu, B., Liu, Y., Lo, C.C., Lozupone, C.A., Dwayne Lunsford, R., Madden, T., Mahurkar, A.A., Mannon, P.J., Mardis, E.R., Markowitz, V.M., Mavromatis, K., McCorrison, J.M., McDonald, D., McEwen, J., McGuire, A.L., McInnes, P., Mehta, T., Mihindukulasuriya, K.A., Miller, J.R., Minx, P.J., Newsham, I., Nusbaum, C., Oglaughlin, M., Orvis, J., Pagani, I., Palaniappan, K., Patel, S.M., Pearson, M., Peterson, J., Podar, M., Pohl, C., Pollard, K.S., Pop, M., Priest, M.E., Proctor, L.M., Qin, X., Raes, J., Ravel, J., Reid, J.G., Rho, M., Rhodes, R., Riehle, K.P., Rivera, M.C., Rodriguez-Mueller, B., Rogers, Y.H., Ross, M.C., Russ, C., Sanka, R.K., Sankar, P., Fah Sathirapongsasuti, J., Schloss, J.A., Schloss, P.D., Schmidt, T.M., Scholz, M., Schriml, L., Schubert, A.M., Segata, N., Segre, J.A., Shannon, W.D., Sharp, R.R., Sharpton, T.J., Shenoy, N., Sheth, N.U., Simone, G.A., Singh, I., Smillie, C.S., Sobel, J.D., Sommer, D.D., Spicer, P., Sutton, G.G., Sykes, S.M., Tabbaa, D.G., Thiagarajan, M., Tomlinson, C.M., Torralba, M., Treangen, T.J., Truty, R.M., Vishnivetskaya, T.A., Walker, J., Wang, L., Wang, Z., Ward, D. V., Warren, W., Watson, M.A., Wellington, C., Wetterstrand, K.A., White, J.R., Wilczek-Boney, K., Wu, Y., Wylie, K.M., Wylie, T., Yandava, C., Ye, L., Ye, Y., Yooseph, S., Youmans, B.P., Zhang, L., Zhou, Y., Zhu, Y., Zoloth, L., Zucker, J.D., Birren, B.W., Gibbs, R.A., Highlander, S.K., Methé, B.A., Nelson, K.E., Petrosino, J.F., Weinstock, G.M., Wilson, R.K. and White, O., 2012. Structure, function and diversity of the healthy human microbiome. Nature 486: 207-214. https://doi.org/10.1038/nature11234
-
Klinder, A., Förster, A., Caderni, G., Angelo, P.F. and Pool-Zobel, B.L., 2004. Fecal water genotoxicity is predictive of tumor-preventive activities by inulin-like oligofructoses, probiotics (Lactobacillus rhamnosus and Bifidobacterium lactis), and their synbiotic combination. Nutrition and Cancer 49: 144-155. https://doi.org/10.1207/s15327914nc4902_5
-
Knekt, P., Adlercreutz, H., Rissanen, H., Aromaa, A., Teppo, L. and Heliövaara, M., 2000. Does antibacterial treatment for urinary tract infection contribute to the risk of breast cancer? British Journal of Cancer 82: 1107. https://doi.org/10.1054/bjoc.1999.1047
-
Kumar, R., Eipers, P., Little, R.B., Crowley, M., Crossman, D.K., Lefkowitz, E.J. and Morrow, C.D., 2014. Getting started with microbiome analysis: sample acquisition to bioinformatics. Current Protocols in Human Genetics 82: 18.8.1-18.8.29. https://doi.org/10.1002/0471142905.hg1808s82
-
Kunik, T., 2001. Genetic transformation of HeLa cells by Agrobacterium. Proceedings of the National Academy of Sciences of the USA 98: 1871-1876. https://doi.org/10.1073/pnas.98.4.1871
-
Li, H., Qi, Y. and Jasper, H., 2016. Preventing age-related decline of gut compartmentalization limits microbiota dysbiosis and extends lifespan. Cell Host and Microbe 19: 240-253. https://doi.org/10.1016/j.chom.2016.01.008
-
Louis, P., Hold, G.L. and Flint, H.J., 2014. The gut microbiota, bacterial metabolites and colorectal cancer. Nature Reviews Microbiology 12: 661-672. https://doi.org/10.1038/nrmicro3344
-
Lu, Y., Fan, C., Li, P., Lu, Y., Chang, X. and Qi, K., 2016. Short chain fatty acids prevent high-fat-diet-induced obesity in mice by regulating g protein-coupled receptors and gut Microbiota. Scientific Reports 6: 37589. https://doi.org/10.1038/srep37589
-
Mantovani, A., 2007. An infernal triangle. Nature 448: 547. https://doi.org/10.1038/448547a
-
Marchesi, J.R., Dutilh, B.E., Hall, N., Peters, W.H.M., Roelofs, R., Boleij, A. and Tjalsma, H., 2011. Towards the human colorectal cancer microbiome. PLoS ONE 6: e20447. https://doi.org/10.1371/journal.pone.0020447
-
Missmer, S.A., Eliassen, A.H., Barbieri, R.L. and Hankinson, S.E., 2004. Endogenous estrogen, androgen, and progesterone concentrations and breast cancer risk among postmenopausal women. Journal of the National Cancer Institute 96: 1856-1865. https://doi.org/10.1093/jnci/djh336
-
Nardone, G. and Compare, D., 2015. The human gastric microbiota: is it time to rethink the pathogenesis of stomach diseases? United European Gastroenterology Journal 3: 255-260. https://doi.org/10.1177/2050640614566846
-
Nicholson, J.K. and Wilson, I.D., 2003. Understanding global systems biology: metabonomics and the continuum of metabolism. Nature Reviews Drug Discovery 2: 668. https://doi.org/10.1038/nrd1157
-
Panthee, S., Hamamoto, H., Paudel, A. and Sekimizu, K., 2016. Lysobacter species: a potential source of novel antibiotics. Archives of Microbiology 198: 839-845. https://doi.org/10.1007/s00203-016-1278-5
-
Plottel, C.S., Blaser, M.J., Kwa, M. and Adams, S., 2016. The intestinal microbiome and estrogen receptor-positive female breast cancer. Journal of the National Cancer Institute 108: djw029. https://doi.org/10.1093/jnci/djw029
-
Rubinstein, M.R., Baik, J.E., Lagana, S.M., Han, R.P., Raab, W.J., Sahoo, D., Dalerba, P., Wang, T.C. and Han, Y.W., 2019. Fusobacterium nucleatum promotes colorectal cancer by inducing Wnt/β-catenin modulator Annexin A1. EMBO reports 20: e47638. https://doi.org/10.15252/embr.201847638
-
Santos-Marcos, J.A., Rangel-Zuñiga, O.A., Jimenez-Lucena, R., Quintana-Navarro, G.M., Garcia-Carpintero, S., Malagon, M.M., Landa, B.B., Tena-Sempere, M., Perez-Martinez, P., Lopez-Miranda, J., Perez-Jimenez, F. and Camargo, A., 2018. Influence of gender and menopausal status on gut microbiota. Maturitas 116: 43-53. https://doi.org/10.1016/j.maturitas.2018.07.008
-
Sauter, C., 1995. Is Hodgkin’s disease a human counterpart of bacterially induced crown-gall tumours? The Lancet 346: 1433. https://doi.org/10.1016/S0140-6736(95)92451-5
-
Seo, I., Jha, B.K., Suh, S.-I., Suh, M.-H. and Baek, W.-K., 2014. Microbial profile of the stomach: comparison between normal mucosa and cancer tissue in the same patient. Journal of Bacteriology and Virology 44: 162-169.
'Microbial profile of the stomach: comparison between normal mucosa and cancer tissue in the same patient ' () 44 Journal of Bacteriology and Virology : 162 -169.
-
Sfanos, K.S., Yegnasubramanian, S., Nelson, W.G. and De Marzo, A.M., 2017. The inflammatory microenvironment and microbiome in prostate cancer development. Nature Reviews Urology 15: 11. https://doi.org/10.1038/nrurol.2017.167
-
Sheflin, A.M., Whitney, A.K. and Weir, T.L., 2014. Cancer-promoting effects of microbial dysbiosis. Current Oncology Reports 16: 406. https://doi.org/10.1007/s11912-014-0406-0
-
Shen, J., Obin, M.S. and Zhao, L., 2013. The gut microbiota, obesity and insulin resistance. Molecular Aspects of Medicine 34: 39-58. https://doi.org/10.1016/j.mam.2012.11.001
-
Siegel, L.R., Miller, D.K. and Jemal, A., 2018. Cancer statistics, 2018. CA: A Cancer Journal for Clinicians 68: 7-30. doi:10.3322/caac.21442
'Cancer statistics, 2018 ' () 68 CA: A Cancer Journal for Clinicians : 7 -30.
-
Sonis, S.T., 2004. The pathobiology of mucositis. Nature Reviews Cancer 4: 277-284. https://doi.org/10.1038/nrc1318
-
Tsuruta, Y., Rogers, L.Q., Krontiras, H., Grizzle, W.E., Fruge, A.D., Oster, R.A., Umphrey, H.R., Jones, L.W., Azrad, M. and Demark-Wahnefried, W., 2016. Exploring effects of presurgical weight loss among women with stage 0-II breast cancer: Protocol for a randomised controlled feasibility trial. BMJ Open 6: e012320. https://doi.org/10.1136/bmjopen-2016-012320
-
Urbaniak, C., Gloor, G.B., Brackstone, M., Scott, L., Tangney, M. and Reida, G., 2016. The microbiota of breast tissue and its association with breast cancer. Applied and Environmental Microbiology American Society for Microbiology, 82: 5039-5048. https://doi.org/10.1128/AEM.01235-16
-
Van der Meulen, T., Harmsen, H., Bootsma, H., Spijkervet, F., Kroese, F. and Vissink, A., 2016. The microbiome-systemic diseases connection. Oral Diseases 22: 719-734. https://doi.org/10.1111/odi.12472
-
Verdam, F.J., Fuentes, S., de Jonge, C., Zoetendal, E.G., Erbil, R., Greve, J.W., Buurman, W.A., de Vos, W.M. and Rensen, S.S., 2013. Human intestinal microbiota composition is associated with local and systemic inflammation in obesity. Obesity 21: E607-E615. https://doi.org/10.1002/oby.20466
-
Vogtmann, E. and Goedert, J.J., 2016. Epidemiologic studies of the human microbiome and cancer. British Journal of Cancer 114: 237. https://doi.org/10.1038/bjc.2015.465
-
World Cancer Research Fund, 2018. Diet, nutrition, physical activity, and cancer: a global perspective. Continuous Update Project Expert Report.
-
World Health Organization, 2018. Global health observatory. World Health Organization, Geneva, Switzerland. Available at: https://who.int/gho/database/en/.
-
Xie, G., Wang, X., Liu, P., Wei, R., Chen, W., Rajani, C., Hernandez, B.Y., Alegado, R., Dong, B., Li, D. and Jia, W., 2016. Distinctly altered gut microbiota in the progression of liver disease. Oncotarget 7: 19355-19366. https://doi.org/10.18632/oncotarget.8466
-
Zhu, P., Baek, S.H., Bourk, E.M., Ohgi, K.A., Garcia-Bassets, I., Sanjo, H., Akira, S., Kotol, P.F., Glass, C.K., Rosenfeld, M.G. and Rose, D.W., 2006. Macrophage/cancer cell interactions mediate hormone resistance by a nuclear receptor derepression pathway. Cell 124: 615-629. https://doi.org/10.1016/j.cell.2005.12.032
-
Zilberstein, B., Quintanilha, A.G., Santos, M.A.A., Pajecki, D., Moura, E.G., Alves, P.R.A., Maluf Filho, F., De Souza, J.A.U. and Gama-Rodrigues, J., 2007. Digestive tract microbiota in healthy volunteers. Clinics 62: 47-54.
'Digestive tract microbiota in healthy volunteers ' () 62 Clinics : 47 -54.
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Gut microbial differences in breast and prostate cancer cases from two randomised controlled trials compared to matched cancer-free controls
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Implicated in several chronic diseases, the gastrointestinal microbiome is hypothesised to influence carcinogenesis. We compared faecal microbiota of newly diagnosed treatment-naïve overweight and obese cancer patients and matched controls. Cases were enrolled in presurgical weight-loss trials for breast (NCT02224807) and prostate (NCT01886677) cancers and had a body mass index (BMI) ≥25 kg/m2. Cancer-free controls were matched 1:1 by age (±5 years), race, gender, and BMI (±5 kg/m2). All participants provided faecal samples; isolated bacterial DNA were PCR amplified at the V4 region of the 16S rRNA gene and analysed using the QIIME pipeline. Tests compared cases versus controls, then separately by gender. Microbial alpha-diversity and beta-diversity were assessed, and relative abundance of Operational Taxonomic Units (OTU’s) were compared at the genus level, with false discovery rate (FDR) correction. 22 overweight and obese cancer patients were matched with 22 cancer-free controls, with an average BMI of 30.5±4.3 kg/m2, age 54.4±5.3 years, and 54.5% were black. Fourteen matches were made between breast cancer cases and healthy female controls, and 8 matches were made with prostate cancer cases and healthy male controls. Comparison of all cases and controls revealed no differences in alpha diversity, though prostate cancer patients had higher Chao1 (P=0.006) and Observed Species (P=0.036) than cancer-free males. Beta-diversity metrics were significantly different between cases and controls (P<0.03 for all tests in whole sample and in men), though only unweighted Unifrac was different in women (P=0.005). Kruskal Wallis tests indicated significant differences among 16 genera in all matches, 9 in female, and 51 in male. This study suggests the faecal microbiota of treatment-naive breast and prostate cancer patients differs from controls, though larger samples are needed to substantiate these findings. Trial registration: NIH Clinical Trials, NCT01886677, NCT02224807, registered 26 June 2013, 25 Aug 2014 (respectively) – retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT01886677; https://clinicaltrials.gov/ct2/show/NCT02224807
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