Save

Analysis of the human intestinal epithelial cell transcriptional response to Lactobacillus acidophilus, Lactobacillus salivarius, Bifidobacterium lactis and Escherichia coli

In: Beneficial Microbes
Authors:
H. Putaala Health & Nutrition, Danisco Sweeteners, Sokeritehtaantie 20, 02460 Kantvik, Finland

Search for other papers by H. Putaala in
Current site
Google Scholar
PubMed
Close
,
R. Barrangou Danisco USA Inc., 3329 Agriculture Drive, Madison, WI 53716, USA

Search for other papers by R. Barrangou in
Current site
Google Scholar
PubMed
Close
,
G. Leyer Danisco USA Inc., 3329 Agriculture Drive, Madison, WI 53716, USA

Search for other papers by G. Leyer in
Current site
Google Scholar
PubMed
Close
,
A. Ouwehand Health & Nutrition, Danisco Sweeteners, Sokeritehtaantie 20, 02460 Kantvik, Finland

Search for other papers by A. Ouwehand in
Current site
Google Scholar
PubMed
Close
,
E. Bech Hansen

Search for other papers by E. Bech Hansen in
Current site
Google Scholar
PubMed
Close
,
D. Romero Danisco USA Inc., 3329 Agriculture Drive, Madison, WI 53716, USA

Search for other papers by D. Romero in
Current site
Google Scholar
PubMed
Close
, and
N. Rautonen Health & Nutrition, Danisco Sweeteners, Sokeritehtaantie 20, 02460 Kantvik, Finland

Search for other papers by N. Rautonen in
Current site
Google Scholar
PubMed
Close
Download Citation Get Permissions

Access options

Get access to the full article by using one of the access options below.

Institutional Login

Log in with Open Athens, Shibboleth, or your institutional credentials

Login via Institution

Purchase

Buy instant access (PDF download and unlimited online access):

€36.93

The complex microbial population residing in the human gastrointestinal tract consists of commensal, potential pathogenic and beneficial species, which are probably perceived differently by the host and consequently could be expected to trigger specific transcriptional responses. Here, we provide a comparative analysis of the global in vitro transcriptional response of human intestinal epithelial cells to Lactobacillus acidophilus NCFM™, Lactobacillus salivarius Ls-33, Bifidobacterium animalis subsp. lactis 420, and enterohaemorrhagic Escherichia coli O157:H7 (EHEC). Interestingly, L. salivarius Ls-33 DCE-induced changes were overall more similar to those of B. lactis 420 than to L. acidophilus NCFM™, which is consistent with previously observed in vivo immunomodulation properties. In the gene ontology and pathway analyses both specific and unspecific changes were observed. Common to all was the regulation of apoptosis and adipogenesis, and lipid-metabolism related regulation by the probiotics. Specific changes such as regulation of cell-cell adhesion by B. lactis 420, superoxide metabolism by L. salivarius Ls-33, and regulation of MAPK pathway by L. acidophilus NCFM™ were noted. Furthermore, fundamental differences were observed between the pathogenic and probiotic treatments in the Toll-like receptor pathway, especially for adapter molecules with a lowered level of transcriptional activation of MyD88, TRIF, IRAK1 and TRAF6 by probiotics compared to EHEC. The results in this study provide insights into the relationship between probiotics and human intestinal epithelial cells, notably with regard to strain-specific responses, and highlight the differences between transcriptional responses to pathogenic and probiotic bacteria.

Content Metrics

All Time Past 365 days Past 30 Days
Abstract Views 646 221 33
Full Text Views 67 3 0
PDF Views & Downloads 79 7 0