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Possible synergistic effect between high lactate and insufficient intake of peptides caused biomass reduction during high-cell starter culture production
in Beneficial MicrobesSearch for other papers by M. Boonmee in
Current site
Google Scholar
Abstract
Lactic starter culture production is normally subjected to the end-product inhibition on growth, which limits the biomass produced per production batch. Removal of lactate ions during the biomass production has improved the biomass production. It allows for the use of higher sugar concentration so that high biomass concentration can be obtained. Lactate removal by ion exchange resin during Lactococcus lactis NZ133 cultivation was applied as a strategy for enhancing the production of lactic starter culture biomass. At high lactose concentrations of 180 g/l, the unexpected reduction in the biomass was evident regardless of the remaining sugar in the fermentation broth. The amount of protein and proteins/polypeptides pattern profile during cultivation were investigated as protein availability was suspected to be the potential cause of biomass reduction during high cell cultivation applying the ion exchange technique. Reduction in biomass concentration, after its maximum of 26 g/l, was observed after the protein concentration was unchanged while the remaining lactose continued to be utilised. A sharp decrease in protein concentration following the addition of resin corresponded to the disappearance of the smear band of protein sized 6,512-26,625 Da when more resin was added to remove lactate. The smear band remained throughout the conventional batch cultivation period. Based on the results, insufficient supply of peptides caused by the loss through adsorption onto ion exchange resin which occurred at high lactate level was postulated as the most probable cause of the biomass reduction. The result also indicated an inefficient use of supplemented protein sources supplied in correspond to the increase in lactose concentration due to the presence of appreciable amounts of residual protein at the end of cultivation process.
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Amrane, A. and Prigent, Y., 1997. Growth and lactic acid production coupling for Lactobacillus helveticus cultivated on supplemented whey: influence of peptidic nitrogen deficiency. Journal of Biotechnology 55: 1-8.
'Growth and lactic acid production coupling for Lactobacillus helveticus cultivated on supplemented whey: influence of peptidic nitrogen deficiency ' () 55 Journal of Biotechnology : 1 -8.
-
Boonmee, M., Leksawasdi, N., Bridge, W. and Rogers, P.L., 2003. Batch and continuous culture of Lactococcus lactis NZ133: experimental data and model development. Biochemical Engineering Journal 14: 127-135.
'Batch and continuous culture of Lactococcus lactis NZ133: experimental data and model development ' () 14 Biochemical Engineering Journal : 127 -135.
-
Boonmee, M., Leksawasdi, N., Bridge, W. and Rogers, P.L., 2006. Electrodialysis for lactate removal in the production of the dairy starter culture Lactococcus lactis NZ133. International Journal of Food Science and Technology 42: 567-572.
'Electrodialysis for lactate removal in the production of the dairy starter culture Lactococcus lactis NZ133 ' () 42 International Journal of Food Science and Technology : 567 -572.
-
Cao, X., Yun, H.S. and Koo, Y.-M., 2002. Recovery of (+)-lactic acid by anion exchange resin Amberlite IRA-400. Biochemical Engineering Journal 11: 189-196.
'Recovery of (+)-lactic acid by anion exchange resin Amberlite IRA-400 ' () 11 Biochemical Engineering Journal : 189 -196.
-
Charbonnel, P., Lamarque, M., Piard, J.-C., Gilbert, C., Juillard, V. and Atlan, D., 2003. Diversity of oligopeptide transport specificity in Lactococcus lactis species. The Journal of Biological Chemistry 278: 14832-14840.
'Diversity of oligopeptide transport specificity in Lactococcus lactis species ' () 278 The Journal of Biological Chemistry : 14832 -14840.
-
Fitzpatrick, J.J. and O'Keeffe, U., 2001. Influence of whey protein hydrolysate addition to whey permeate batch fermentations for producing lactic acid. Process Biochemistry 37: 183-186.
'Influence of whey protein hydrolysate addition to whey permeate batch fermentations for producing lactic acid ' () 37 Process Biochemistry : 183 -186.
-
Guimont, Ch., 2002. Change of free amino acids in M17 medium after growth of Streptococcus thermophilus and identification of a glutamine transport ATP-binding protein. International Dairy Journal 12: 729-736.
'Change of free amino acids in M17 medium after growth of Streptococcus thermophilus and identification of a glutamine transport ATP-binding protein ' () 12 International Dairy Journal : 729 -736.
-
Juillard, V., Guillot, A., Le Bars, D. and Gripon, J.-C., 1998. Specificity of milk peptide utilization by Lactococcus lactis. Applied and Environmental Microbiology 64: 1230-1236.
-
Juillard, V., Laan, H., Kunji, E.R., Jeronimus-Stratingh, C.M., Bruins, A.P. and Konings, W.N., 1995a. The extracellular PI-type proteinase of Lactococcus lactis hydrolyzes beta-casein into more than one hundred different oligopeptides. Journal of Bacteriology 177: 3472-3478.
'The extracellular PI-type proteinase of Lactococcus lactis hydrolyzes beta-casein into more than one hundred different oligopeptides ' () 177 Journal of Bacteriology : 3472 -3478.
-
Juillard, V., Le Bars, D., Kunji, E.R., Konings, W.N., Gripon, J.C. and Richard, J., 1995b. Oligopeptides are the main source of nitrogen for Lactococcus lactis during growth in milk. Applied and Environmental Microbiology 61: 3024-3030.
'Oligopeptides are the main source of nitrogen for Lactococcus lactis during growth in milk ' () 61 Applied and Environmental Microbiology : 3024 -3030.
-
Kunji, E.R.S., Hagting, A., De Vries, C.J., Juillard, V., Haandrikman, A.J., Poolman, B. and Konings, W.N., 1995. Transport of β-casein-derived peptides by the oligopeptide transport system is a crucial step in the proteolytic pathway of Lactococcus lactis. The Journal of Biological Chemistry 270: 1569-1574.
-
Letort, C., Nardi, M., Garault, P., Monnet, V. and Juillard, V., 2002. Casein utilization by Streptococcus thermophilus results in a diauxic growth in milk. Applied and Environmental Microbiology 68: 3162-3165.
'Casein utilization by Streptococcus thermophilus results in a diauxic growth in milk ' () 68 Applied and Environmental Microbiology : 3162 -3165.
-
Li, H., Mustacchi, R., Knowles, C.J., Skibar, W., Sunderland, G., Dalrymple, I. and Jackman, S.A., 2004. An electrokinetic bioreactor: using direct electric current for enhanced lactic acid fermentation and product recovery. Tetrahedron 60: 655-661.
'An electrokinetic bioreactor: using direct electric current for enhanced lactic acid fermentation and product recovery ' () 60 Tetrahedron : 655 -661.
-
Luedeking, R. and Piret, E.L., 1959. A kinetic study of the lactic acid fermentation. Journal of Biochemical and Microbiological Technology and Engineering 1: 393-412.
'A kinetic study of the lactic acid fermentation ' () 1 Journal of Biochemical and Microbiological Technology and Engineering : 393 -412.
-
Moldes, A.B., Alonso, J.L. and Parajó, J.C., 2003. Recovery of lactic acid from simultaneous saccharification and fermentation media using anion exchange resins. Bioprocess and Biosystems Engineering 25: 357-363.
'Recovery of lactic acid from simultaneous saccharification and fermentation media using anion exchange resins ' () 25 Bioprocess and Biosystems Engineering : 357 -363.
-
Norton, S., Lacroix, C. and Vuillemard, J.G., 1994. Reduction of yeast extract supplementation in lactic acid fermentation of whey permeate by immobilized cell technology. Journal of Dairy Science 77: 2494-2508.
'Reduction of yeast extract supplementation in lactic acid fermentation of whey permeate by immobilized cell technology ' () 77 Journal of Dairy Science : 2494 -2508.
-
Østlie, H., Floberghagen, V., Reinbold, G., Hammond E.G., Vegarud, G. and Langsrud, T., 1995. Autolysis of dairy propionibacteria: growth studies, peptidase activities, and proline production. Journal of Dairy Science 78: 1224-1237.
'Autolysis of dairy propionibacteria: growth studies, peptidase activities, and proline production ' () 78 Journal of Dairy Science : 1224 -1237.
-
Schägger, H., 2006. Tricine-SDS-PAGE. Nature Protocols 1: 16-22.
'Tricine-SDS-PAGE ' () 1 Nature Protocols : 16 -22.
-
Van Asseldonk, M.G.J., De Vos, W.M. and Simons, A.F.M., 1991. DNA fragment from Lactococcus lactis and fusion proteins thereof. European Patent Application EP0455280A1. 27 March 1991.
-
Venkatesh, K.V., Okos, M.R. and Wankat, P.C., 1993. Kinetic model of growth and lactic acid production from lactose by Lactobacillus bulgaricus. Process Biochemistry 28: 231-241.
-
Yankov, D., Molinier, J., Albet, J., Malmary, G. and Kyuchoukov, G., 2004. Lactic acid extraction from aqueous solutions with tri-n-octylamine dissolved in decanol and dodecane. Biochemical Engineering Journal 21: 63-71.
'Lactic acid extraction from aqueous solutions with tri-n-octylamine dissolved in decanol and dodecane ' () 21 Biochemical Engineering Journal : 63 -71.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 136 | 73 | 12 |
| Gesamttextansichten | 5 | 0 | 0 |
| PDF-Downloads | 13 | 0 | 0 |
Possible synergistic effect between high lactate and insufficient intake of peptides caused biomass reduction during high-cell starter culture production
in Beneficial MicrobesSearch for other papers by M. Boonmee in
Current site
Google Scholar
PubMed
Abstract
Lactic starter culture production is normally subjected to the end-product inhibition on growth, which limits the biomass produced per production batch. Removal of lactate ions during the biomass production has improved the biomass production. It allows for the use of higher sugar concentration so that high biomass concentration can be obtained. Lactate removal by ion exchange resin during Lactococcus lactis NZ133 cultivation was applied as a strategy for enhancing the production of lactic starter culture biomass. At high lactose concentrations of 180 g/l, the unexpected reduction in the biomass was evident regardless of the remaining sugar in the fermentation broth. The amount of protein and proteins/polypeptides pattern profile during cultivation were investigated as protein availability was suspected to be the potential cause of biomass reduction during high cell cultivation applying the ion exchange technique. Reduction in biomass concentration, after its maximum of 26 g/l, was observed after the protein concentration was unchanged while the remaining lactose continued to be utilised. A sharp decrease in protein concentration following the addition of resin corresponded to the disappearance of the smear band of protein sized 6,512-26,625 Da when more resin was added to remove lactate. The smear band remained throughout the conventional batch cultivation period. Based on the results, insufficient supply of peptides caused by the loss through adsorption onto ion exchange resin which occurred at high lactate level was postulated as the most probable cause of the biomass reduction. The result also indicated an inefficient use of supplemented protein sources supplied in correspond to the increase in lactose concentration due to the presence of appreciable amounts of residual protein at the end of cultivation process.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 136 | 73 | 12 |
| Gesamttextansichten | 5 | 0 | 0 |
| PDF-Downloads | 13 | 0 | 0 |
