Feeding and condition shifts after encountering a pathogen
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Abstract
Feeding behaviour is a dynamic process, especially if an individual is dealing with an infection. Here, we used Tenebrio molitor beetles to evaluate the effects of changes in diet macronutrients (protein:carbohydrate) on: (i) feeding behaviour before and after infection (using the entomopathogenic fungus Metarhizium robertsii) in males; and (ii) body condition, measured as the amount of proteins, carbohydrates, and lipids in the body, in males and females. Given that females also depend on the nutrients from the spermatophore, we also addressed the impact on female condition of using spermatophores from males whose diets differed in macronutrients whether they were confronting an infection. We found that males with different diets and regardless of their infection status, and females with different diets, all consumed less of the protein-rich diet but more of the carbohydrate-rich diet. In addition, infection in males produced anorexia. The infection resulted in males and the females they mated with, with fewer body proteins and lipids. This suggests that unlike studies in other insects, T. molitor does not consume large amounts of protein during the adult stage, even during an infection. Femalesâ condition depended strongly on that of their mates, improving even when paired with infected males. This implies that females may be using the nutrients that the males transfer during mating for maintenance.
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-
Adamo, S.A. (2012). The effects of the stress response on immune function in invertebrates: an evolutionary perspective on an ancient connection. â Horm. Behav. 62: 324-330.
-
Adamo, S.A., Fidler, T.L. & Forestell, C.A. (2007). Illness-induced anorexia and its possible function in the caterpillar, Manduca sexta. â Brain. Behav. Immun. 21: 292-300.
-
Adamo, S.A., Bartlett, A., Le, J., Spencer, N. & Sullivan, K. (2010). Illness-induced anorexia may reduce trade-offs between digestion and immune function. â Anim. Behav. 79: 3-10.
-
Alaux, C., Ducloz, F., Crauser, D. & Le Conte, Y. (2010). Diet effects on honeybee immunocompetence. â Biol. Lett. 6: 562-565.
-
Aubert, A. (1999). Sickness and behaviour in animals: a motivational perspective. â Neurosci. Biobehav. Rev. 23: 1029-1036.
-
Ayres, J.S. & Schneider, D.S. (2009). The role of anorexia in resistance and tolerance to infections in Drosophila. â PLoS Biol. 7: e1000150.
-
Behmer, S.T. (2009). Insect herbivore nutrient regulation. â Annu. Rev. Entomol. 54: 165-187.
-
Berthoud, H.-R. & Seeley, R.J. (1999). Neural and metabolic control of macronutrient intake. â CRC Press, Boca Raton, FL.
-
Bhattacharya, A.K., Ameel, J.J. & Waldbauer, G.P. (1970). A method for sexing living pupal and adult yellow mealworms. â Ann. Entomol. Soc. Am. 63: 1783.
-
Bonduriansky, R. & Chenoweth, S.F. (2009). Intralocus sexual conflict. â Trends Ecol. Evol. 24: 280-288.
-
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. â Anal. Biochem. 72: 248-254.
-
Bunning, H., Bassett, L., Clowser, C., Rapkin, J., Jensen, K., House, C.M., Archer, C.R. & Hunt, J. (2016). Dietary choice for a balanced nutrient intake increases the mean and reduces the variance in the reproductive performance of male and female cockroaches. â Ecol. Evol. 6: 4711-4730.
-
Cahenzli, F. & Erhardt, A. (2013). Nectar amino acids enhance reproduction in male butterflies. â Oecologia 171: 197-205.
-
Carazo, P., Fernández-Perea, R. & Font, E. (2012). Quantity estimation based on numerical cues in the mealworm beetle (Tenebrio molitor). â Front. Psychol. 3: 502.
-
Chandra, R.K. (1996). Nutrition, immunity and infection: from basic knowledge of dietary manipulation of immune responses to practical application of ameliorating suffering and improving survival. â Proc. Natl. Acad. Sci. USA 93: 14304-14307.
-
Chapman, R.F., Simpson, S.J. & Douglas, A.E. (2013). The insects: structure and function. â Cambridge University Press, Cambridge.
-
Chown, S.L. & Nicolson, S. (2004). Insect physiological ecology: mechanisms and patterns. â Oxford University Press, Oxford.
-
Clutton-Brock, T.H. (1984). Reproductive effort and terminal investment in iteroparous animals. â Am. Nat. 123: 212-229.
-
Cordes, N., Albrecht, F., Engqvist, L., Schmoll, T., Baier, M., Müller, C. & Reinhold, K. (2015). Larval food composition affects courtship song and sperm expenditure in a lekking moth. â Ecol. Entomol. 40: 34-41.
-
Cotter, S.C., Simpson, S.J., Raubenheimer, D. & Wilson, K. (2011). Macronutrient balance mediates trade-offs between immune function and life history traits. â Funct. Ecol. 25: 186-198.
-
Dadd, R.H. (1960). The nutritional requirements of locusts â I development of synthetic diets and lipid requirements. â J. Insect Physiol. 4: 319-347.
-
Dantzer, R. (2004). Cytokine-induced sickness behaviour: a neuroimmune response to activation of innate immunity. â Eur. J. Pharmacol. 500: 399-411.
-
Dantzer, R. & Kelley, K.W. (1989). Stress and immunity: an integrated view of relationships between the brain and the immune system. â Life Sci. 44: 1995-2008.
-
Drnevich, J.M., Papke, R.S., Rauser, C.L. & Rutowski, R.L. (2001). Material benefits from multiple mating in female mealworm beetles (Tenebrio molitor L.). â J. Insect Behav. 14: 215-230.
-
Duffield, K.R., Hampton, K.J., Houslay, T.M., Rapkin, J., Hunt, J., Sadd, B.M. & Sakaluk, S.K. (2020). Macronutrient intake and simulated infection threat independently affect life history traits of male decorated crickets. â Ecol. Evol. 10: 11766-11778.
-
Dunn, P.E., Bohnert, T.J. & Russell, V. (1994). Regulation of antibacterial protein synthesis following infection and during metamorphosis of Manduca sexta. â Ann. N.Y. Acad. Sci. 712: 117-130.
-
Dussutour, A., Latty, T., Beekman, M. & Simpson, S.J. (2010). Amoeboid organism solves complex nutritional challenges. â Proc. Natl. Acad. Sci. USA 107: 4607-4611.
-
Engebretson, J.A. & Mason, W.H. (1980). Transfer of 65 Zn at mating in Heliothis virescens. â Environ. Entomol. 9: 119-121.
-
Engqvist, L. (2005). The mistreatment of covariate interaction terms in linear model analyses of behavioural and evolutionary ecology studies. â Anim. Behav. 70: 967-971.
-
Ferkau, C. & Fischer, K. (2006). Costs of reproduction in male Bicyclus anynana and Pieris napi butterflies: effects of mating history and food limitation. â Ethology 112: 1117-1127.
-
Foray, V., Pelisson, P., Bel-venner, M., Desouhant, E., Venner, S., Menu, F., Giron, D. & Rey, B. (2012). A handbook for uncovering the complete energetic budget in insects: the van Handelâs method (1985) revisited. â Physiol. Entomol. 37: 295-302.
-
Fox, J. & Weisberg, S. (2019). An R companion to applied regression, 3rd edn. â Sage, Thousand Oaks, CA.
-
Fraenkel, G. (1950). The nutrition of the mealworm, Tenebrio molitor L.(Tenebrionidae, Coleoptera). â Physiol. Zool. 23: 92-108.
-
Freitak, D., Ots, I., Vanatoa, A. & Hörak, P. (2003). Immune response is energetically costly in white cabbage butterfly pupae. â Proc. Roy. Soc. Lond. B: Biol. Sci. 270: S220-S222.
-
Gerber, G.H. (1976). Reproductive behaviour and physiology of Tenebrio molitor (Coleoptera: Tenebrionidae). III. Histogenetic changes in the internal genitalia, mesenteron, and cuticle during sexual maturation. â Can. J. Zool. 54: 990-1002.
-
Goettel, M.S. & Inglis, G.D. (1997). Fungi: hyphomycetes. â In: Manual of techniques in insect pathology (Lacey, L., ed.). Elsevier Science, Amsterdam, p. 213-249.
-
Graves, S., Piepho, H.-P. & Selzer, M.L. (2015). Package âmultcompViewâ. â Vis. Paired Comp.
-
Grover, C.D., Kay, A.D., Monson, J.A., Marsh, T.C. & Holway, D.A. (2007). Linking nutrition and behavioural dominance: carbohydrate scarcity limits aggression and activity in Argentine ants. â Proc. Roy. Soc. Lond. B: Biol. Sci. 274: 2951-2957.
-
Gwynne, D.T. (2008). Sexual conflict over nuptial gifts in insects. â Annu. Rev. Entomol. 53: 83-101.
-
Harrison, S.J., Raubenheimer, D., Simpson, S.J., Godin, J.-G.J. & Bertram, S.M. (2014). Towards a synthesis of frameworks in nutritional ecology: interacting effects of protein, carbohydrate and phosphorus on field cricket fitness. â Proc. Roy. Soc. Lond. B: Biol. Sci. 281: 20140539.
-
Hart, B.L. (1988). Biological basis of the behavior of sick animals. â Neurosci. Biobehav. Rev. 12: 123-137.
-
Heller, K.G., Faltin, S., Fleischmann, P. & Helversen, O.V. (1998). The chemical composition of the spermatophore in some species of phaneropterid bushcrickets (Orthoptera: Tettigonioidea). â J. Insect Physiol. 44: 1001-1008.
-
Hurd, H. & Ardin, R. (2003). Infection increases the value of nuptial gifts, and hence male reproductive success, in the Hymenolepis diminuta-Tenebrio molitor association. â Proc. Roy. Soc. Lond. B: Biol. Sci. 270: S172-S174.
-
Jensen, K., Mayntz, D., Toft, S., Clissold, F.J., Hunt, J., Raubenheimer, D. & Simpson, S.J. (2012). Optimal foraging for specific nutrients in predatory beetles. â Proc. Roy. Soc. Lond. B: Biol. Sci. 279: 2212-2218.
-
Jensen, K., McClure, C., Priest, N.K. & Hunt, J. (2015). Sex-specific effects of protein and carbohydrate intake on reproduction but not lifespan in Drosophila melanogaster. â Aging Cell 14: 605-615.
-
Karlsson, B. (1995). Resource allocation and mating systems in butterflies. â Evolution 49: 955-961.
-
Kazlauskas, N., Klappenbach, M., Depino, A.M. & Locatelli, F.F. (2016). Sickness behavior in honey bees. â Front. Physiol. 7: 261.
-
Kivleniece, I., Krams, I., DaukÅ¡te, J., Krama, T. & Rantala, M.J. (2010). Sexual attractiveness of immune-challenged male mealworm beetles suggests terminal investment in reproduction. â Anim. Behav. 80: 1015-1021.
-
Knell, R.J. & Webberley, K.M. (2004). Sexually transmitted diseases of insects: distribution, evolution, ecology and host behaviour. â Biol. Rev. 79: 557-581.
-
Krams, I.A., Krama, T., Moore, F.R., Kivleniece, I., Kuusik, A., Freeberg, T.M., Mänd, R., Rantala, M.J., DaukÅ¡te, J. & Mänd, M. (2014). Male mealworm beetles increase resting metabolic rate under terminal investment. â J. Evol. Biol. 27: 541-550.
-
Kuo, T.-H., Pike, D.H., Beizaeipour, Z. & Williams, J.A. (2010). Sleep triggered by an immune response in Drosophila is regulated by the circadian clock and requires the NFκB Relish. â BMC Neurosci. 11: 1-12.
-
Kyriazakis, I., Emmans, G.C. & Whittemore, C.T. (1991). The ability of pigs to control their protein intake when fed in three different ways. â Phys. Behav. 50: 1197-1203.
-
Kyriazakis, I., Tolkamp, B.J. & Hutchings, M.R. (1998). Towards a functional explanation for the occurrence of anorexia during parasitic infections. â Anim. Behav. 56: 265-274.
-
Lazzaro, B.P. & Little, T.J. (2009). Immunity in a variable world. â Philos. Trans. Roy. Soc. Lond. B Biol. Sci. 364: 15-26.
-
Lee, K.P., Cory, J.S., Wilson, K., Raubenheimer, D. & Simpson, S.J. (2006). Flexible diet choice offsets protein costs of pathogen resistance in a caterpillar. â Proc. Roy. Soc. Lond. B: Biol. Sci. 273: 823-829.
-
Lee, K.P., Simpson, S.J., Clissold, F.J., Brooks, R., Ballard, J.W.O., Taylor, P.W., Soran, N. & Raubenheimer, D. (2008). Lifespan and reproduction in Drosophila: new insights from nutritional geometry. â Proc. Natl. Acad. Sci. USA 105: 2498-2503.
-
Leger, R.J.S., Butt, T.M., Goettel, M.S., Staples, R.C. & Roberts, D.W. (1989). Productionin vitro of appressoria by the entomopathogenic fungus Metarhizium anisopliae. â Exp. Mycol. 13: 274-288.
-
Lenth, R. & Lenth, M.R. (2018). Package âlsmeansâ. â Am. Stat. 34: 216-221.
-
Lin, L., Fang, W., Liao, X., Wang, F., Wei, D. & Leger, R.J.S. (2011). The MrCYP52 cytochrome P450 monoxygenase gene of Metarhizium robertsii is important for utilizing insect epicuticular hydrocarbons. â PLoS ONE 6: e28984.
-
Lochmiller, R.L. & Deerenberg, C. (2000). Trade-offs in evolutionary immunology: just what is the cost of immunity? â Oikos 88: 87-98.
-
Maklakov, A.A., Simpson, S.J., Zajitschek, F., Hall, M.D., Dessmann, J., Clissold, F., Raubenheimer, D., Bonduriansky, R. & Brooks, R.C. (2008). Sex-specific fitness effects of nutrient intake on reproduction and lifespan. â Curr. Biol. 18: 1062-1066.
-
Muller, K., Thiéry, D., Moret, Y. & Moreau, J. (2015). Male larval nutrition affects adult reproductive success in wild European grapevine moth (Lobesia botrana). â Behav. Ecol. Sociobiol. 69: 39-47.
-
Nielsen, M.L. & Holman, L. (2012). Terminal investment in multiple sexual signals: immune-challenged males produce more attractive pheromones. â Funct. Ecol. 26: 20-28.
-
Povey, S., Cotter, S.C., Simpson, S.J. & Wilson, K. (2014). Dynamics of macronutrient self-medication and illness-induced anorexia in virally infected insects. â J. Anim. Ecol. 83: 245-255.
-
R Core Team (2017). R: a language and environment for statistical com-puting. â R Found. Stat. Comput. Vienna, available online at https//www.R-project.org.
-
Rapkin, J., Jensen, K., Archer, C.R., House, C.M., Sakaluk, S.K., Del Castillo, E. & Hunt, J. (2018). The geometry of nutrient space-based life-history trade-offs: sex-specific effects of macronutrient intake on the trade-off between encapsulation ability and reproductive effort in decorated crickets. â Am. Nat. 191: 452-474.
-
Raubenheimer, D. & Simpson, S.J. (1997). Integrative models of nutrient balancing: application to insects and vertebrates. â Nutr. Res. Rev. 10: 151-179.
-
Raubenheimer, D. & Simpson, S.J. (2018). Nutritional ecology and foraging theory. â Curr. Opin. Insect Sci. 27: 38-45.
-
Raubenheimer, D., Lee, K.P. & Simpson, S.J. (2005). Does Bertrandâs rule apply to macronutrients? â Proc. Roy. Soc. Lond. B: Biol. Sci. 272: 2429-2434.
-
Raubenheimer, D., Simpson, S.J. & Mayntz, D. (2009). Nutrition, ecology and nutritional ecology: toward an integrated framework. â Funct. Ecol. 23: 4-16.
-
Reddiex, A.J., Gosden, T.P., Bonduriansky, R. & Chenoweth, S.F. (2013). Sex-specific fitness consequences of nutrient intake and the evolvability of diet preferences. â Am. Nat. 182: 91-102.
-
Reyes-RamÃrez, A., Rocha-Ortega, M. & Córdoba-Aguilar, A. (2019a). Female preferences when female condition and male ornament expression vary. â Biol. J. Linn. Soc. 128: 828-837.
-
Reyes-RamÃrez, A., EnrÃquez-Vara, J.N., Rocha-Ortega, M., Téllez-GarcÃa, A. & Córdoba-Aguilar, A. (2019b). Female choice for sick males over healthy males: consequences for offspring. â Ethology. 125: 241-249.
-
Reyes-RamÃrez, A., Rocha-Ortega, M. & Córdoba-Aguilar, A. (2021). Dietary macronutrient balance and fungal infection as drivers of spermatophore quality in the mealworm beetle. â Curr. Res. Insect Sci.: 100009.
-
Rho, M.S. & Lee, K.P. (2014). Geometric analysis of nutrient balancing in the mealworm beetle, Tenebrio molitor L.(Coleoptera: Tenebrionidae). â J. Insect Phys. 71: 37-45.
-
Rodrigues, M.A., Martins, N.E., Balancé, L.F., Broom, L.N., Dias, A.J.S., Fernandes, A.S.D., Rodrigues, F., Sucena, Ã. & Mirth, C.K. (2015). Drosophila melanogaster larvae make nutritional choices that minimize developmental time. â J. Insect Physiol. 81: 69-80.
-
Sadd, B., Holman, L., Armitage, H., Lock, F., Marland, R. & Siva-Jothy, M.T. (2006). Modulation of sexual signalling by immune challenged male mealworm beetles (Tenebrio molitor, L.): evidence for terminal investment and dishonesty. â J. Evol. Biol. 19: 321-325.
-
Scheiner, S.M. (1993). MANOVA: multiple response variables and multispecies interactions. â In: Design and analysis of ecological experiments (Scheiner, S.M. & Gurevitch, J., eds). Chapman & Hall, New York, NY, p. 94-112.
-
Simpson, S.J. & Abisgold, J.D. (1985). Compensation by locusts for changes in dietary nutrients: behavioural mechanisms. â Physiol. Entomol. 10: 443-452.
-
Simpson, S.J. & Raubenheimer, D. (1993). A multi-level analysis of feeding behaviour: the geometry of nutritional decisions. â Philos. Trans. Roy. Soc. Lond. B: Biol. Sci. 342: 381-402.
-
Simpson, S.J. & Raubenheimer, D. (1999). Assuaging nutritional complexity: a geometrical approach. â Proc. Nutr. Soc. 58: 779-789.
-
Simpson, S.J. & Raubenheimer, D. (2012). The nature of nutrition: a unifying framework. â Aust. J. Zool. 59: 350-368.
-
Simpson, S.J., Batley, R. & Raubenheimer, D. (2003). Geometric analysis of macronutrient intake in humans: the power of protein? â Appetite 41: 123-140.
-
Simpson, S.J., Sibly, R.M., Lee, K.P., Behmer, S.T. & Raubenheimer, D. (2004). Optimal foraging when regulating intake of multiple nutrients. â Anim. Behav. 68: 1299-1311.
-
Simpson, S.J., Le Couteur, D.G., Raubenheimer, D., Solon-Biet, S.M., Cooney, G.J., Cogger, V.C. & Fontana, L. (2017). Dietary protein, aging and nutritional geometry. â Ageing Res. Rev. 39: 78-86.
-
Simpson, S.J., Ribeiro, C. & González-Tokman, D. (2018). Feeding behavior. â In: Insect behaviour from mechanical to ecological evolution consequences (Córdoba-Aguilar, A., González-Tokman, D. & González-Santoyo, I., eds). Oxford University Press, Oxford, p. 416.
-
Singer, M.S., Mace, K.C. & Bernays, E.A. (2009). Self-medication as adaptive plasticity: increased ingestion of plant toxins by parasitized caterpillars. â PLoS ONE 4: e4796.
-
South, S.H., House, C.M., Moore, A.J., Simpson, S.J. & Hunt, J. (2011). Male cockroaches prefer a high carbohydrate diet that makes them more attractive to females: implications for the study of condition dependence. â Evol. Int. J. Org. Evol. 65: 1594-1606.
-
St, L., Joshi, L., Bidochka, M.J., Rizzo, N.W. & Roberts, D.W. (1996). Characterization and ultrastructural localization of chitinases from Metarhizium anisopliae, M. flavoviride, and Beauveria bassiana during fungal invasion of host (Manduca sexta) cuticle. â Appl. Environ. Microbiol. 62: 907-912.
-
Stanley-Samuelson, D.W. & Loher, W. (1983). Arachidonic and other long-chain polyunsaturated fatty acids in spermatophores and spermathecae of Teleogryllus commodus: significance in prostaglandin-mediated reproductive behaviour. â J. Insect Physiol. 29: 41-45.
-
Tye, S.P., Blaske, B.K. & Siepielski, A.M. (2020). Population-level variation of digestive physiology costs of mounting an immune response in damselflies. â Ecol. Entomol. 45: 635-643.
-
Van Handel, E. (1985). Rapid determination of total lipids in mosquitoes. â J. Am. Mosq. Control Ass. 1: 302-304.
-
Van Handel, E. & Day, J.F. (1988). Assay of lipids, glycogen and sugars in individual mosquitoes: correlations with wing length in field-collected Aedes vexans. â J. Am. Mosq. Control Ass. 4: 549-550.
-
Voigt, C.C., Lehmann, G.U.C., Michener, R.H. & Joachimski, M.M. (2006). Nuptial feeding is reflected in tissue nitrogen isotope ratios of female katydids. â Funct. Ecol. 20: 656-661.
-
Waldbauer, G.P. & Friedman, S. (1991). Self-selection of optimal diets by insects. â Annu. Rev. Entomol. 36: 43-63.
-
Wang, C. & Leger, R.J.S. (2005). Developmental and transcriptional responses to host and nonhost cuticles by the specific locust pathogen Metarhizium anisopliae var. acridum. â Eukaryot. Cell 4: 937-947.
-
Wang, C. & Leger, R.J.S. (2007). The Metarhizium anisopliae perilipin homolog MPL1 regulates lipid metabolism, appressorial turgor pressure, and virulence. â J. Biol. Chem. 282: 21110-21115.
-
Watanabe, M. & Sato, K. (1993). A spermatophore structured in the bursa copulatrix of the small white Pieris rapae (Lepidoptera, Pieridae) during copulation, and its sugar content. â J Res Lepid 32: 26-36.
-
Weaver, D.K. & McFarlane, J.E. (1990). The effect of larval density on growth and development of Tenebrio molitor. â J. Insect Physiol. 36: 531-536.
-
Weers, P.M.M. & Ryan, R.O. (2006). Apolipophorin III: role model apolipoprotein. â Insect Biochem. Mol. Biol. 36: 231-240.
-
Worden, B.D. & Parker, P.G. (2001). Polyandry in grain beetles, Tenebrio molitor, leads to greater reproductive success: material or genetic benefits? â Behav. Ecol. 12: 761-767.
-
Zajitschek, F., Hunt, J., Zajitschek, S.R.K., Jennions, M.D. & Brooks, R. (2007). No intra-locus sexual conflict over reproductive fitness or ageing in field crickets. â PLoS ONE 2: e155.
-
Zhong, W., McClure, C.D., Evans, C.R., Mlynski, D.T., Immonen, E., Ritchie, M.G. & Priest, N.K. (2013). Immune anticipation of mating in Drosophila: Turandot M promotes immunity against sexually transmitted fungal infections. â Proc. Roy. Soc. Lond. B: Biol. Sci. 280: 20132018.
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|---|---|---|---|
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Feeding and condition shifts after encountering a pathogen
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Abstract
Feeding behaviour is a dynamic process, especially if an individual is dealing with an infection. Here, we used Tenebrio molitor beetles to evaluate the effects of changes in diet macronutrients (protein:carbohydrate) on: (i) feeding behaviour before and after infection (using the entomopathogenic fungus Metarhizium robertsii) in males; and (ii) body condition, measured as the amount of proteins, carbohydrates, and lipids in the body, in males and females. Given that females also depend on the nutrients from the spermatophore, we also addressed the impact on female condition of using spermatophores from males whose diets differed in macronutrients whether they were confronting an infection. We found that males with different diets and regardless of their infection status, and females with different diets, all consumed less of the protein-rich diet but more of the carbohydrate-rich diet. In addition, infection in males produced anorexia. The infection resulted in males and the females they mated with, with fewer body proteins and lipids. This suggests that unlike studies in other insects, T. molitor does not consume large amounts of protein during the adult stage, even during an infection. Femalesâ condition depended strongly on that of their mates, improving even when paired with infected males. This implies that females may be using the nutrients that the males transfer during mating for maintenance.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 739 | 154 | 5 |
| å ¨ææµè§æ¬¡æ° | 27 | 0 | 0 |
| PDFä¸è½½æ¬¡æ° | 54 | 0 | 0 |
