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Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals
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An increasing incidence of T-2 and HT-2 toxins (T-2 and HT-2) representing group A trichothecenes has been observed in Fusarium infected small grain cereals in the last decade. Quite recently, the existence of glucosides of T-2 and HT-2 has also been proved next to the common parent forms. There is a strong desire to deepen the knowledge of the occurrence of these conjugates and the possibilities of their analytical determination. A new analytical procedure has been developed for monitoring T-2/HT-2 conjugates in cereal samples. Four different sample preparation methods based on crude acetonitrile:water extraction, QuEChERS, solid phase extraction and immunoaffinity clean-up, were tested. The latter approach employing dedicated immunoaffinity cross-reactive cartridges was shown to be the best option for selective isolation and pre-concentration of the target conjugated analytes. The samples obtained in this way were examined by ultra-high performance liquid chromatography hyphenated to high resolution tandem mass spectrometry that enabled the confirmation of the presence of conjugated T-2 and HT-2. In addition to mono-glycosylated forms of T-2 and HT-2 detected in naturally contaminated barley, wheat and oats, we have also documented for the first time the existence of diglucosides of HT-2 in barley.
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Berthiller, F., Krska, R., Domig, K.J., Kneifel, W., Juge, N., Schuhmacher, R. and Adam, G., 2011. Hydrolytic fate of deoxynivalenol-3-glucoside during digestion. Toxicology Letters 206: 264-267.
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| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 289 | 60 | 9 |
| Gesamttextansichten | 53 | 1 | 0 |
| PDF-Downloads | 16 | 0 | 0 |
Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals
in World Mycotoxin JournalSearch for other papers by Z. Veprikova in
Current site
Google Scholar
PubMed
Search for other papers by M. Vaclavikova in
Current site
Google Scholar
PubMed
Search for other papers by O. Lacina in
Current site
Google Scholar
PubMed
Search for other papers by Z. Dzuman in
Current site
Google Scholar
PubMed
Search for other papers by M. Zachariasova in
Current site
Google Scholar
PubMed
Search for other papers by J. Hajslova in
Current site
Google Scholar
PubMed
An increasing incidence of T-2 and HT-2 toxins (T-2 and HT-2) representing group A trichothecenes has been observed in Fusarium infected small grain cereals in the last decade. Quite recently, the existence of glucosides of T-2 and HT-2 has also been proved next to the common parent forms. There is a strong desire to deepen the knowledge of the occurrence of these conjugates and the possibilities of their analytical determination. A new analytical procedure has been developed for monitoring T-2/HT-2 conjugates in cereal samples. Four different sample preparation methods based on crude acetonitrile:water extraction, QuEChERS, solid phase extraction and immunoaffinity clean-up, were tested. The latter approach employing dedicated immunoaffinity cross-reactive cartridges was shown to be the best option for selective isolation and pre-concentration of the target conjugated analytes. The samples obtained in this way were examined by ultra-high performance liquid chromatography hyphenated to high resolution tandem mass spectrometry that enabled the confirmation of the presence of conjugated T-2 and HT-2. In addition to mono-glycosylated forms of T-2 and HT-2 detected in naturally contaminated barley, wheat and oats, we have also documented for the first time the existence of diglucosides of HT-2 in barley.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 289 | 60 | 9 |
| Gesamttextansichten | 53 | 1 | 0 |
| PDF-Downloads | 16 | 0 | 0 |
