Multi-mycotoxin determination methodology

Jean-Yves Pierard; Christophe Depasse; Arnout Delafortrie; Jean-Claude Motte

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Multi-mycotoxin determination methodology

In: Meeting the mycotoxin menace

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Jean-Yves Pierard

Jean-Yves Pierard
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Christophe Depasse

Christophe Depasse
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Arnout Delafortrie

Arnout Delafortrie
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Jean-Claude Motte

Jean-Claude Motte
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Type:: Chapter

Pages:: 255–268

DOI:: https://doi.org/10.3920/978-90-8686-523-9_016

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This paper describes the methodology used as a tool for the development of multi-mycotoxin analysis methods able to identify and quantify simultaneously several mycotoxins of different structures or families. The main steps are the liquid extraction using a solvent mixture of acetonitrile and water (84-16%, v/v), the purification using both ion exchange column and Mycosep columns, the HPLC analysis on a C-18 column and the double detection using both UV and fluorescence, respectively. As they are all nonspecific each step has to be optimized.

The currently most relevant mycotoxins in food and animal feed such as nivalenol, deoxynivalenol, 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, zearalenone, zearalenol and ochratoxin A, have been investigated following the developed procedure and the first results obtained on samples are quite satisfactory.

This procedure, when completed, will offer a complete screening of mycotoxins likely to be present in sample batches.

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Meeting the mycotoxin menace

E-Book ISBN:: 9789086865239

Publisher:: Wageningen Academic

Print Publication Date:: 10 Oct 2004

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- Life Sciences

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Multi-mycotoxin determination methodology

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Meeting the mycotoxin menace

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