Enzymatic hydrolysis of black soldier fly larvae oil enhances antimicrobial and antioxidant properties across different strains
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Abstract
Black soldier fly larvae (BSFL) present a sustainable solution for converting organic waste into value-added biomass. However, the bioactive potential of oil extracted from the larvae remains underexplored. This study characterised BSFL oil composition from diverse genetic strains reared on different substrates, focusing on the fatty acid profiles as well as antimicrobial and antioxidant activities via enzymatic hydrolysis. Overall proximate analysis revealed that moisture, ash, protein, oil and carbohydrate contents ranged from 2.22-4.44%, 6.30-16.97%, 31.74-47.13%, 18.78-50.03% and 5.35-17.73%, respectively. Specifically, fatty acid profile was dominated by lauric (C12:0), palmitic (C16:0), oleic (C18:1), and linoleic acids (C18:2), with relative compositions significantly influences by both substrate type and genetic strain. Whole-cell biocatalyst (WCB) from Rhizopus oryzae exhibited lipase activity of 134.44 U/g, and acheived 79.63-87.67% hydrolysis, releasing free fatty acids. Notably, hydrolysis enhanced antimicrobial activity against Bacillus cereus (6.80-12.11 mm to 9.38-16.61 mm) and Pseudomonas aeruginosa (6.97-10.20 mm to 8.10-12.00 mm), while maintaining antioxidant activity (IC50: 35.3-108.2 mg/ml in extracts; 28.37-109.65 mg/ml in hydrolysates). These findings suggest enzymatic hydrolysis is an effective strategy to unlock the bioactive compounds in BSFL oil, positioning it as a promising natural antimicrobial and antioxidant source for feed and food applications.
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Supplementary Materials
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Enzymatic hydrolysis of black soldier fly larvae oil enhances antimicrobial and antioxidant properties across different strains
In: Journal of Insects as Food and FeedSearch for other papers by S.A. Putri in
Current site
Google Scholar
PubMed
Search for other papers by M.Z. Arrazi in
Current site
Google Scholar
PubMed
Search for other papers by P. Unadirekkul in
Current site
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PubMed
Search for other papers by Q.-H. Zhang in
Current site
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PubMed
Search for other papers by Á. Kenéz in
Current site
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PubMed
Search for other papers by R.E. Putra in
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PubMed
Search for other papers by L. Melani in
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PubMed
Search for other papers by N. Puniamoorthy in
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PubMed
Search for other papers by M.Y. Abduh in
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PubMed
Abstract
Black soldier fly larvae (BSFL) present a sustainable solution for converting organic waste into value-added biomass. However, the bioactive potential of oil extracted from the larvae remains underexplored. This study characterised BSFL oil composition from diverse genetic strains reared on different substrates, focusing on the fatty acid profiles as well as antimicrobial and antioxidant activities via enzymatic hydrolysis. Overall proximate analysis revealed that moisture, ash, protein, oil and carbohydrate contents ranged from 2.22-4.44%, 6.30-16.97%, 31.74-47.13%, 18.78-50.03% and 5.35-17.73%, respectively. Specifically, fatty acid profile was dominated by lauric (C12:0), palmitic (C16:0), oleic (C18:1), and linoleic acids (C18:2), with relative compositions significantly influences by both substrate type and genetic strain. Whole-cell biocatalyst (WCB) from Rhizopus oryzae exhibited lipase activity of 134.44 U/g, and acheived 79.63-87.67% hydrolysis, releasing free fatty acids. Notably, hydrolysis enhanced antimicrobial activity against Bacillus cereus (6.80-12.11 mm to 9.38-16.61 mm) and Pseudomonas aeruginosa (6.97-10.20 mm to 8.10-12.00 mm), while maintaining antioxidant activity (IC50: 35.3-108.2 mg/ml in extracts; 28.37-109.65 mg/ml in hydrolysates). These findings suggest enzymatic hydrolysis is an effective strategy to unlock the bioactive compounds in BSFL oil, positioning it as a promising natural antimicrobial and antioxidant source for feed and food applications.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 506 | 506 | 59 |
| Full Text Views | 18 | 18 | 2 |
| PDF Views & Downloads | 55 | 55 | 6 |
