Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Gut commensal Bacteroides faecichinchillae, a potential novel candidate of next-generation probiotics targeting type 2 diabetes
in Beneficial MicrobesSearch for other papers by M. Godet in
Current site
Google Scholar
Search for other papers by E. Delmas in
Current site
Google Scholar
Search for other papers by C. GeÌrard in
Current site
Google Scholar
Search for other papers by E. Meugnier in
Current site
Google Scholar
Search for other papers by A. Bravard in
Current site
Google Scholar
Search for other papers by C. Pinteur in
Current site
Google Scholar
Search for other papers by N. Vega in
Current site
Google Scholar
Search for other papers by N. Bendridi in
Current site
Google Scholar
Service de Biochimie et Biologie MoleÌculaire, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, 69500 Bron, France
Search for other papers by D. Cheillan in
Current site
Google Scholar
Search for other papers by S. Fourati in
Current site
Google Scholar
Search for other papers by D. Rainteau in
Current site
Google Scholar
Search for other papers by M. Le Barz in
Current site
Google Scholar
Search for other papers by S. Binda in
Current site
Google Scholar
Search for other papers by F. Leulier in
Current site
Google Scholar
Search for other papers by A. Bernalier-Donadille in
Current site
Google Scholar
Search for other papers by H. Vidal in
Current site
Google Scholar
Abstract
The implication of gut microbiota in the pathophysiology of type 2 diabetes, one of the major health concerns worldwide, has demonstrated the benefits of using probiotics to improve dysbiosis. Facing the lack of defined research strategy to found and qualify bacterial with real antidiabetic activity, we have developed a program to identify, select and validate novel strains able to improve metabolic alterations in type 2 diabetes. Among potential candidates, we identified Bacteroides faecichinchillae (named BAfa hereafter), and the present work presents the validation of the antidiabetic potential of the ST37 (DSMZ 26883) BAfa strain in mice fed a high-fat high-sucrose (HFS) diet for 10 weeks followed by 4 weeks of treatment. Daily gavage with live, but not heat inactivated, BAfa improved glucose tolerance, fasting hyperinsulinemia and liver triglyceride content in HFS fed mice. Furthermore, these beneficial effects were retained after lyophilization. Using this preclinical model, we characterised BAfa mechanism of action, showing that four weeks of treatment was associated with reduced hepatic accumulation of ceramides, key actors of insulin resistance. The underlying mechanism could be related to BAfa-induced changes in the intestinal bile acid profile and inhibition of the Fxr-Fgf15 pathway in the ileum. BAfa supplementation during 4 weeks in HFS fed mice was also associated with mild modifications of gut microbiota composition, with the upregulation of several species known for their beneficial metabolic actions (such as Lactobacillus johnsonii, Limosilactobacillus reutei, Roseburia, Turicimonas muris, Phocaeicola dorei or Akkermansia muciniphila) and for being up-regulated during metformin treatment. Altogether, these data indicate that BAfa and related B. faecichinchillae strains could be considered for developing next-generation probiotics to treat type 2 diabetes, potentially in combination with the anti-diabetic drug metformin.
Kauf
Sofortzugang erwerben (PDF-Download und unbegrenzter Online-Zugang):
Institutszugang
Melden Sie sich mit Open Athens, Shibboleth oder Ihren institutionellen Anmeldedaten an.
Persönliche Anmeldung
Melden Sie sich mit Ihrem brill.com-Konto an
-
Al-Fakhrany, O.M. and Elekhnawy, E., 2024. Next-generation probiotics: the upcoming biotherapeutics. Molecular Biology Reports 51: 505. https://doi.org/10.1007/s11033-024-09398-5
-
Beau, A., Benoit, B., Le Barz, M., Meugnier, E., Penhoat, A., Calzada, C., Pinteur, C., Loizon, E., Chanon, S., Vieille-Marchiset, A., Sauvinet, V., Godet, M., Laugerette, F., Holowacz, S., Jacouton, E., Michalski, M.-C. and Vidal, H., 2023. Inhibition of intestinal FXR activity as a possible mechanism for the beneficial effects of a probiotic mix supplementation on lipid metabolism alterations and weight gain in mice fed a high fat diet. Gut Microbes 15: 2281015. https://doi.org/10.1080/19490976.2023.2281015
-
Bravard, A., GeÌrard, C., Defois, C., Benoit, B., Makki, K., Meugnier, E., Rainteau, D., Rieusset, J., Godet, M. and Vidal, H., 2021. Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice. Science Reports 11: 16684. https://doi.org/10.1038/s41598-021-95117-0
-
Brown, E.M., Ke, X., Hitchcock, D., Jeanfavre, S., Avila-Pacheco, J., Nakata, T., Arthur, T.D., Fornelos, N., Heim, C., Franzosa, E.A., Watson, N., Huttenhower, C., Haiser, H.J., Dillow, G., Graham, D.B., Finlay, B.B., Kostic, A.D., Porter, J.A., Vlamakis, H., Clish, C.B. and Xavier, R.J., 2019. Bacteroides-derived sphingolipids are critical for maintaining intestinal homeostasis and symbiosis. Cell Host and Microbe 25: 668-680.e7. https://doi.org/10.1016/j.chom.2019.04.002
-
Cani, P.D., 2014. The gut microbiota manages host metabolism. Nature Reviews Endocrinology 10: 74-76. https://doi.org/10.1038/nrendo.2013.240
-
Choi, K.J., Yoon, M.Y., Kim, J.-E. and Yoon, S.S., 2023. Gut commensal Kineothrix alysoides mitigates liver dysfunction by restoring lipid metabolism and gut microbial balance. Science Reports 13: 14668. https://doi.org/10.1038/s41598-023-41160-y
-
Clarke, K.R. and Owens, N.J.P., 1983. A simple and versatile micro-computer program for the determination of âmost probable numberâ. Journal of Microbiological Methods 1: 133-137. https://doi.org/10.1016/0167-7012(83)90031-3
-
Clement, K., LeRoy, T., Debadat, J. and Voland, L., 2022. Composition and use of Turicimonas muris for the treatment of metabolic diseases. PCT/FR2022/052482.
-
Da Costa, W.K.A., BrandaÌo, L.R., Martino, M.E., Garcia, E.F., Alves, A.F., de Souza, E.L., de Souza Aquino, J., Saarela, M., Leulier, F., Vidal, H. and Magnani, M., 2019. Qualification of tropical fruit-derived Lactobacillus plantarum strains as potential probiotics acting on blood glucose and total cholesterol levels in Wistar rats. Food Research International 124: 109-117. https://doi.org/10.1016/j.foodres.2018.08.035
-
de Carvalho, M.B., Jorge, G.M.C.P., Zanardo, L.W., Hamada, L.M., Izabel, L. dos S., Santoro, S. and Magdalon, J., 2024. The role of FGF19 in metabolic regulation: insights from preclinical models to clinical trials. American Journal of Physiology â Endocrinology and Metabolism 327: E279-E289. https://doi.org/10.1152/ajpendo.00156.2024
-
Depommier, C., Everard, A., Druart, C., Plovier, H., Van Hul, M., Vieira-Silva, S., Falony, G., Raes, J., Maiter, D., Delzenne, N.M., de Barsy, M., Loumaye, A., Hermans, M.P., Thissen, J.-P., de Vos, W.M. and Cani, P.D., 2019. Supplementation with Akkermansia muciniphila in overweight and obese human volunteers: a proof-of-concept exploratory study. Nature Medicine 25: 1096-1103. https://doi.org/10.1038/s41591-019-0495-2
-
FaÌk, F. and Bäckhed, F., 2012. Lactobacillus reuteri prevents diet-induced obesity, but not atherosclerosis, in a strain dependent fashion in Apoeâ/â mice. PLoS ONE 7: e46837. https://doi.org/10.1371/journal.pone.0046837
-
GarcıÌa-LoÌpez, M., Meier-Kolthoff, J.P., Tindall, B.J., Gronow, S., Woyke, T., Kyrpides, N.C., Hahnke, R.L. and Göker, M., 2019. Analysis of 1,000 type-strain genomes improves taxonomic classification of Bacteroidetes. Frontiers in Microbiology 10: 2083. https://doi.org/10.3389/fmicb.2019.02083
-
Gauffin Cano, P., Santacruz, A., Moya, AÌ. and Sanz, Y., 2012. Bacteroides uniformis CECT 7771 ameliorates metabolic and immunological dysfunction in mice with high-fat-diet induced obesity. PLoS ONE 7: e41079. https://doi.org/10.1371/journal.pone.0041079
-
GeÌrard, C. and Vidal, H., 2019. Impact of gut microbiota on host glycemic control. Frontiers in Endocrinology 10: 29. https://doi.org/10.3389/fendo.2019.00029
-
GeÌrard, P., Lepercq, P., Leclerc, M., Gavini, F., Raibaud, P. and Juste, C., 2007. Bacteroides sp. strain D8, the first cholesterol-reducing bacterium isolated from human feces. Applied and Environmental Microbiology 73: 5742-5749. https://doi.org/10.1128/AEM.02806-06
-
Global status report on noncommunicable diseases, 2014. World Health Organization WHO/NMH/NVI/15.1
-
Godet, M., Meugnier, E., Vitalis, O., Bendridi, N., Vieille-Marchiset, A., Vega, N., Benoit, B., Pinteur, C., Rainteau, D., Cheillan, D., Michalski, M.-C., Chikh, K. and Vidal, H., 2025. Evaluation of the effects of metformin on gut functions and microbiota and their contribution to improving glucose tolerance in diabetic mice. Molecular Metabolism 102: 102263. https://doi.org/10.1016/j.molmet.2025.102263
-
Hammerschmidt, P. and Brüning, J.C., 2022. Contribution of specific ceramides to obesity-associated metabolic diseases. Cellular and Molecular Life Sciences 79: 395. https://doi.org/10.1007/s00018-022-04401-3
-
Hsieh, F.-C., Lee, C.-L., Chai, C.-Y., Chen, W.-T., Lu, Y.-C. and Wu, C.-S., 2013. Oral administration of Lactobacillus reuteri GMNL-263 improves insulin resistance and ameliorates hepatic steatosis in high fructose-fed rats. Nutrition and Metabolism 10: 35. https://doi.org/10.1186/1743-7075-10-35
-
Humbert, L., Maubert, M.A., Wolf, C., Duboc, H., MaheÌ, M., Farabos, D., Seksik, P., Mallet, J.M., Trugnan, G., Masliah, J. and Rainteau, D., 2012. Bile acid profiling in human biological samples: comparison of extraction procedures and application to normal and cholestatic patients. Journal of Chromatography B 899: 135-145. https://doi.org/10.1016/j.jchromb.2012.05.015
-
Jiang, C., Xie, C., Lv, Y., Li, J., Krausz, K.W., Shi, J., Brocker, C.N., Desai, D., Amin, S.G., Bisson, W.H., Liu, Y., Gavrilova, O., Patterson, A.D. and Gonzalez, F.J., 2015. Intestine-selective farnesoid X receptor inhibition improves obesity-related metabolic dysfunction. Nature Communications 6: 10166. https://doi.org/10.1038/ncomms10166
-
Johnson, E.L., Heaver, S.L., Waters, J.L., Kim, B.I., Bretin, A., Goodman, A.L., Gewirtz, A.T., Worgall, T.S. and Ley, R.E., 2020. Sphingolipids produced by gut bacteria enter host metabolic pathways impacting ceramide levels. Nature Communications 11: 2471. https://doi.org/10.1038/s41467-020-16274-w
-
Karlsson, F.H., Tremaroli, V., Nookaew, I., Bergström, G., Behre, C.J., Fagerberg, B., Nielsen, J. and Bäckhed, F., 2013. Gut metagenome in European women with normal, impaired and diabetic glucose control. Nature 498: 99-103. https://doi.org/10.1038/nature12198
-
Kasai, C., Sugimoto, K., Moritani, I., Tanaka, J., Oya, Y., Inoue, H., Tameda, M., Shiraki, K., Ito, M., Takei, Y. and Takase, K., 2015. Comparison of the gut microbiota composition between obese and non-obese individuals in a Japanese population, as analyzed by terminal restriction fragment length polymorphism and next-generation sequencing. BMC Gastroenterology 15: 100. https://doi.org/10.1186/s12876-015-0330-2
-
Kitahara, M., Sakamoto, M., Tsuchida, S., Kawasumi, K., Amao, H., Benno, Y. and Ohkuma, M., 2012. Bacteroides stercorirosoris sp. nov. and Bacteroides faecichinchillae sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces. International Journal of Systematic and Evolutionary Microbiology 62: 1145-1150. https://doi.org/10.1099/ijs.0.032706-0
-
Le Barz, M., Vors, C., Combe, E., Joumard-Cubizolles, L., Lecomte, M., Joffre, F., Trauchessec, M., Pesenti, S., Loizon, E., Breyton, A.-E., Meugnier, E., Bertrand, K., Drai, J., Robert, C., Durand, A., Cuerq, C., Gaborit, P., Leconte, N., Bernalier-Donadille, A., Cotte, E., Laville, M., Lambert-Porcheron, S., Ouchchane, L., Vidal, H., Malpuech-BrugeÌre, C., Cheillan, D. and Michalski, M.-C., 2021. Milk polar lipids favorably alter circulating and intestinal ceramide and sphingomyelin species in postmenopausal women. JCI Insight 6: e146161. https://doi.org/10.1172/jci.insight.146161
-
Le, H.H., Lee, M.-T., Besler, K.R. and Johnson, E.L., 2022. Host hepatic metabolism is modulated by gut microbiotaâderived sphingolipids. Cell Host and Microbe 30: 798-808.e7. https://doi.org/10.1016/j.chom.2022.05.002
-
Lee, H., An, J., Kim, J., Choi, D., Song, Y., Lee, C.-K., Kong, H., Kim, S.B. and Kim, K., 2022. A novel bacterium, Butyricimonas virosa, preventing HFD-induced diabetes and metabolic disorders in mice via GLP-1 receptor. Frontiers in Microbiology 13: 858192. https://doi.org/10.3389/fmicb.2022.858192
-
Ley, R.E., Turnbaugh, P.J., Klein, S. and Gordon, J.I., 2006. Human gut microbes associated with obesity. Nature 444: 1022-1023. https://doi.org/10.1038/4441022a
-
Li, Y., Wang, L., Yi, Q., Luo, L. and Xiong, Y., 2024. Regulation of bile acids and their receptor FXR in metabolic diseases. Frontiers in Nutrition 11: 1447878. https://doi.org/10.3389/fnut.2024.1447878
-
Natividad, J.M., Agus, A., Planchais, J., Lamas, B., Jarry, A.C., Martin, R., Michel, M.-L., Chong-Nguyen, C., Roussel, R., Straube, M., Jegou, S., McQuitty, C., Le Gall, M., Da Costa, G., Lecornet, E., Michaudel, C., Modoux, M., Glodt, J., Bridonneau, C., Sovran, B., Dupraz, L., Bado, A., Richard, M.L., Langella, P., Hansel, B., Launay, J.-M., Xavier, R.J., Duboc, H. and Sokol, H., 2018. Impaired aryl hydrocarbon receptor ligand production by the gut microbiota is a key factor in metabolic syndrome. Cell Metabolism 28: 737-749.e4. https://doi.org/10.1016/j.cmet.2018.07.001
-
Plovier, H., Everard, A., Druart, C., Depommier, C., Van Hul, M., Geurts, L., Chilloux, J., Ottman, N., Duparc, T., Lichtenstein, L., Myridakis, A., Delzenne, N.M., Klievink, J., Bhattacharjee, A., van der Ark, K.C.H., Aalvink, S., Martinez, L.O., Dumas, M.-E., Maiter, D., Loumaye, A., Hermans, M.P., Thissen, J.-P., Belzer, C., de Vos, W.M. and Cani, P.D., 2017. A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice. Nature Medicine 23: 107-113. https://doi.org/10.1038/nm.4236
-
Qin, J., Li, Y., Cai, Z., Li, S., Zhu, J., Zhang, F., Liang, S., Zhang, W., Guan, Y., Shen, D., Peng, Y., Zhang, D., Jie, Z., Wu, W., Qin, Y., Xue, W., Li, J., Han, L., Lu, D., Wu, P., Dai, Y., Sun, X., Li, Z., Tang, A., Zhong, S., Li, X., Chen, W., Xu, R., Wang, M., Feng, Q., Gong, M., Yu, J., Zhang, Y., Zhang, M., Hansen, T., Sanchez, G., Raes, J., Falony, G., Okuda, S., Almeida, M., LeChatelier, E., Renault, P., Pons, N., Batto, J.-M., Zhang, Z., Chen, H., Yang, R., Zheng, W., Li, S., Yang, H., Wang, J., Ehrlich, S.D., Nielsen, R., Pedersen, O., Kristiansen, K. and Wang, J., 2012. A metagenome-wide association study of gut microbiota in type 2 diabetes. Nature 490: 55-60. https://doi.org/10.1038/nature11450
-
Ruan, Y., Sun, J., He, J., Chen, F., Chen, R. and Chen, H., 2015. Effect of probiotics on glycemic control: a systematic review and meta-analysis of randomized, controlled trials. PLoS ONE 10: e0132121. https://doi.org/10.1371/journal.pone.0132121
-
Shin, N.-R., Lee, J.-C., Lee, H.-Y., Kim, M.-S., Whon, T.W., Lee, M.-S. and Bae, J.-W., 2014. An increase in the Akkermansia spp. population induced by metformin treatment improves glucose homeostasis in diet-induced obese mice. Gut 63: 727-735. https://doi.org/10.1136/gutjnl-2012-303839
-
Sokolowska, E. and Blachnio-Zabielska, A., 2019. The Role of Ceramides in Insulin Resistance. Front. Endocrinol. 10. https://doi.org/10.3389/fendo.2019.00577
-
Sun, L., Xie, C., Wang, G., Wu, Y., Wu, Q., Wang, X., Liu, J., Deng, Y., Xia, J., Chen, B., Zhang, S., Yun, C., Lian, G., Zhang, X., Zhang, H., Bisson, W.H., Shi, J., Gao, X., Ge, P., Liu, C., Krausz, K.W., Nichols, R.G., Cai, J., Rimal, B., Patterson, A.D., Wang, X., Gonzalez, F.J. and Jiang, C., 2018. Gut microbiota and intestinal FXR mediate the clinical benefits of metformin. Nature Medicine 24: 1919-1929. https://doi.org/10.1038/s41591-018-0222-4
-
Tan, H., Zhai, Q. and Chen, W., 2019. Investigations of Bacteroides spp. towards next-generation probiotics. Food Research International 116: 637-644. https://doi.org/10.1016/j.foodres.2018.08.088
-
Turnbaugh, P.J., Bäckhed, F., Fulton, L. and Gordon, J.I., 2008. Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Cell Host Microbe 3: 213-223. https://doi.org/10.1016/j.chom.2008.02.015
-
Turnbaugh, P.J., Ley, R.E., Mahowald, M.A., Magrini, V., Mardis, E.R. and Gordon, J.I., 2006. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444: 1027-1031. https://doi.org/10.1038/nature05414
-
Won, S.-M., Chen, S., Lee, S.Y., Lee, K.E., Park, K.W. and Yoon, J.-H., 2020. Lactobacillus sakei ADM14 induces anti-obesity effects and changes in gut microbiome in high-fat diet-induced obese mice. Nutrients 12: 3703. https://doi.org/10.3390/nu12123703
-
Wong, S.K., Chin, K.-Y., Suhaimi, F.H., Fairus, A. and Ima-Nirwana, S., 2016. Animal models of metabolic syndrome: a review. Nutrition and Metabolism 13: 65. https://doi.org/10.1186/s12986-016-0123-9
-
Wu, F., Guo, Y., Wang, Y., Sui, X., Wang, H., Zhang, H., Xin, B., Yang, C., Zhang, C., Jiang, S., Qu, L., Feng, Q., Dai, Z., Shi, C. and Li, Y., 2024. Effects of long-term fasting on gut microbiota, serum metabolome, and their association in male adults. Nutrients 17: 35. https://doi.org/10.3390/nu17010035
-
Wu, H., Esteve, E., Tremaroli, V., Khan, M.T., Caesar, R., ManneraÌs-Holm, L., StaÌhlman, M., Olsson, L.M., Serino, M., Planas-FeÌlix, M., Xifra, G., Mercader, J.M., Torrents, D., Burcelin, R., Ricart, W., Perkins, R., FernaÌndez-Real, J.M. and Bäckhed, F., 2017. Metformin alters the gut microbiome of individuals with treatment-naive type 2 diabetes, contributing to the therapeutic effects of the drug. Nature Medicine 23: 850-858. https://doi.org/10.1038/nm.4345
-
Xie, C., Jiang, C., Shi, J., Gao, X., Sun, D., Sun, L., Wang, T., Takahashi, S., Anitha, M., Krausz, K.W., Patterson, A.D. and Gonzalez, F.J., 2016. An intestinal farnesoid X receptorâceramide signaling axis modulates hepatic gluconeogenesis in mice. Diabetes 66: 613-626. https://doi.org/10.2337/db16-0663
-
Xin, J., Zeng, D., Wang, H., Ni, X., Yi, D., Pan, K. and Jing, B., 2014. Preventing non-alcoholic fatty liver disease through Lactobacillus johnsonii BS15 by attenuating inflammation and mitochondrial injury and improving gut environment in obese mice. Applied Microbiology and Biotechnology 98: 6817-6829. https://doi.org/10.1007/s00253-014-5752-1
-
Xu, X., Wang, Y., Wu, X., Cai, T., Dong, L., Liang, S., Zhu, L., Song, X., Dong, Y., Zheng, Y., Li, L. and Sun, W., 2024. Administration of Alistipes indistinctus prevented the progression from nonalcoholic fatty liver disease to nonalcoholic steatohepatitis by enhancing the gut barrier and increasing Lactobacillus spp. Biochemical and Biophysical Research Communications 741: 151033. https://doi.org/10.1016/j.bbrc.2024.151033
-
Yao, K., Zeng, L., He, Q., Wang, W., Lei, J. and Zou, X., 2017. Effect of probiotics on glucose and lipid metabolism in type 2 diabetes mellitus: a meta-analysis of 12 randomized controlled trials. Medical Science Monitor 23: 3044-3053. https://doi.org/10.12659/MSM.902600
-
Zhang, C., Ma, K., Nie, K., Deng, M., Luo, W., Wu, X., Huang, Y. and Wang, X., 2022. Assessment of the safety and probiotic properties of Roseburia intestinalis: A potential âNext Generation Probioticâ. Frontiers in Microbiology 13: 973046. https://doi.org/10.3389/fmicb.2022.973046
-
Zhang, J., Zhou, J., He, Z. and Li, H., 2024. Bacteroides and NAFLD: pathophysiology and therapy. Frontiers in Microbiology 15: 1288856. https://doi.org/10.3389/fmicb.2024.1288856
-
Zhou, H., Huang, D., Sun, Z. and Chen, X., 2024. Effects of intestinal Desulfovibrio bacteria on host health and its potential regulatory strategies: A review. Microbiology Research 284: 127725. https://doi.org/10.1016/j.micres.2024.127725
-
Zhu, Y., Chen, B., Zhang, X., Akbar, M.T., Wu, T., Zhang, Y., Zhi, L. and Shen, Q., 2024. Exploration of the Muribaculaceae family in the gut microbiota: diversity, metabolism, and function. Nutrients 16: 2660. https://doi.org/10.3390/nu16162660
Supplementary Materials
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 120 | 120 | 120 |
| Gesamttextansichten | 3 | 3 | 3 |
| PDF-Downloads | 9 | 9 | 9 |
Gut commensal Bacteroides faecichinchillae, a potential novel candidate of next-generation probiotics targeting type 2 diabetes
in Beneficial MicrobesSearch for other papers by M. Godet in
Current site
Google Scholar
PubMed
Search for other papers by E. Delmas in
Current site
Google Scholar
PubMed
Search for other papers by C. GeÌrard in
Current site
Google Scholar
PubMed
Search for other papers by E. Meugnier in
Current site
Google Scholar
PubMed
Search for other papers by A. Bravard in
Current site
Google Scholar
PubMed
Search for other papers by C. Pinteur in
Current site
Google Scholar
PubMed
Search for other papers by N. Vega in
Current site
Google Scholar
PubMed
Search for other papers by N. Bendridi in
Current site
Google Scholar
PubMed
Service de Biochimie et Biologie MoleÌculaire, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, 69500 Bron, France
Search for other papers by D. Cheillan in
Current site
Google Scholar
PubMed
Search for other papers by S. Fourati in
Current site
Google Scholar
PubMed
Search for other papers by D. Rainteau in
Current site
Google Scholar
PubMed
Search for other papers by M. Le Barz in
Current site
Google Scholar
PubMed
Search for other papers by S. Binda in
Current site
Google Scholar
PubMed
Search for other papers by F. Leulier in
Current site
Google Scholar
PubMed
Search for other papers by A. Bernalier-Donadille in
Current site
Google Scholar
PubMed
Search for other papers by H. Vidal in
Current site
Google Scholar
PubMed
Abstract
The implication of gut microbiota in the pathophysiology of type 2 diabetes, one of the major health concerns worldwide, has demonstrated the benefits of using probiotics to improve dysbiosis. Facing the lack of defined research strategy to found and qualify bacterial with real antidiabetic activity, we have developed a program to identify, select and validate novel strains able to improve metabolic alterations in type 2 diabetes. Among potential candidates, we identified Bacteroides faecichinchillae (named BAfa hereafter), and the present work presents the validation of the antidiabetic potential of the ST37 (DSMZ 26883) BAfa strain in mice fed a high-fat high-sucrose (HFS) diet for 10 weeks followed by 4 weeks of treatment. Daily gavage with live, but not heat inactivated, BAfa improved glucose tolerance, fasting hyperinsulinemia and liver triglyceride content in HFS fed mice. Furthermore, these beneficial effects were retained after lyophilization. Using this preclinical model, we characterised BAfa mechanism of action, showing that four weeks of treatment was associated with reduced hepatic accumulation of ceramides, key actors of insulin resistance. The underlying mechanism could be related to BAfa-induced changes in the intestinal bile acid profile and inhibition of the Fxr-Fgf15 pathway in the ileum. BAfa supplementation during 4 weeks in HFS fed mice was also associated with mild modifications of gut microbiota composition, with the upregulation of several species known for their beneficial metabolic actions (such as Lactobacillus johnsonii, Limosilactobacillus reutei, Roseburia, Turicimonas muris, Phocaeicola dorei or Akkermansia muciniphila) and for being up-regulated during metformin treatment. Altogether, these data indicate that BAfa and related B. faecichinchillae strains could be considered for developing next-generation probiotics to treat type 2 diabetes, potentially in combination with the anti-diabetic drug metformin.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 120 | 120 | 120 |
| Gesamttextansichten | 3 | 3 | 3 |
| PDF-Downloads | 9 | 9 | 9 |
