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Immunoaffinity clean-up of mycotoxins with organic solvent-free elution
in World Mycotoxin JournalSearch for other papers by J. Stroka in
Current site
Google Scholar
Search for other papers by J. Seidler in
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A novel immunoaffinity (IA) clean-up procedure for the isolation of mycotoxins from maize extracts is described. Commonly, mycotoxins are eluted with organic solvents from IA columns after isolation. The new procedure uses water at 70 °C as alternative. The resulting eluate is suitable for direct and complete injection onto a reversed-phase liquid-chromatography column. Evaporation, reconstitution or dilution are not required. The procedure has been tested for a variety of mycotoxins, including deoxynivalenol (DON) zearalenone, T-2 and HT-2 toxins, aflatoxins and ochratoxin A in an array of different matrices. The example of DON in maize is discussed in more detail and evaluated for repeatability (5%, n=10) and recovery (95%, n=10).
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| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 181 | 79 | 16 |
| Gesamttextansichten | 19 | 5 | 0 |
| PDF-Downloads | 24 | 12 | 0 |
Immunoaffinity clean-up of mycotoxins with organic solvent-free elution
in World Mycotoxin JournalSearch for other papers by J. Stroka in
Current site
Google Scholar
PubMed
Search for other papers by J. Seidler in
Current site
Google Scholar
PubMed
A novel immunoaffinity (IA) clean-up procedure for the isolation of mycotoxins from maize extracts is described. Commonly, mycotoxins are eluted with organic solvents from IA columns after isolation. The new procedure uses water at 70 °C as alternative. The resulting eluate is suitable for direct and complete injection onto a reversed-phase liquid-chromatography column. Evaporation, reconstitution or dilution are not required. The procedure has been tested for a variety of mycotoxins, including deoxynivalenol (DON) zearalenone, T-2 and HT-2 toxins, aflatoxins and ochratoxin A in an array of different matrices. The example of DON in maize is discussed in more detail and evaluated for repeatability (5%, n=10) and recovery (95%, n=10).
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 181 | 79 | 16 |
| Gesamttextansichten | 19 | 5 | 0 |
| PDF-Downloads | 24 | 12 | 0 |
