Evolution of some biochemical markers of growth in relation to osteoarticular status in young horses: results of a longitudinal study in three breeds
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Abstract
Osteocalcin (OC), bone fraction of alkaline phosphatases (BAP) and hydroxyproline (HOP) are markers of bone cell activity. The kinetics of these markers and the analysis of their variations could be related to the osteoarticular status (OAS) of young horses. The growth of Thoroughbreds, French Trotters and Selle Franc̨ais horses was followed up to 18 months. Blood samples were taken regularly to measure OC, HOP and BAP by standardized techniques. The OAS was evaluated by radiographic examination of the limbs. Based on radiographic findings, two groups of horses were investigated, with no lesions or severely affected. Analysis of variance was used to detect the effects of age and breed, and OAS on parameters. The logarithmic model was used to determine the kinetics of the markers. A rapid decrease in marker concentrations with age and differences between breed was observed. At birth, BAP, OC and HOP concentrations were significantly higher in normal horses (1910 UI l−1, 192 ng ml−1 and 35 mg l−1, respectively) than in horses with severe lesions (1620 UI l−1, 149 ng ml−1 and 24 mg l−1, respectively). During the first 6 months, OC, HOP and BAP remained lower in severely affected horses.
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McIlwraith CW (2005). Use of synovial fluid and serum biomarkers in equine bone and joint disease: a review. Equine Veterinary Journal 37: 473–482.
-
Price PA and Baukol SA (1980). 125-dihydroxyvitamin D3 increases synthesis of the vitamin K-dependent bone protein by osteosarcoma cells. Journal of Biological Chemistry 255: 11660–11663.
-
Davicco MJ, Faulconnier Y, Coxam V, Dubroeucq H, Martin- Rosset W and Barlet JP (1994). Systemic bone growth factors in light breed mares and their foals. Archives Internationales de Physiologie, de Biochimie et de Biophysique 102: 115–119.
-
Mäenpää PH, Pirskanen A and Koskinen E (1988). Biochemical indicators of bone formation in foal after transfer from pasture to stables for the winter months. American Journal of Veterinary Research 49: 1990–1992.
-
Glade M, Gupta S and Reimers TJ (1984). Hormonal responses to high and low planes of nutrition in weanling Thouroughbreds. Journal of Animal Science 5: 658–665.
-
McIlwraith CW (1986). Proceedings of the AQHA Developmental Orthopaedic Disease Symposium. Amarillo, TX: AQHA, pp. 1–77.
-
Jeffcott LB and Henson FMD (1998). Studies on growth cartilage in the horse and their application to aetiopathogenesis of dyschondroplasia (osteochondrosis). The Veterinary Journal 156: 177–192.
-
Jeffcott LB (2005). Developmental diseases affecting growing horse. The Growing Horse: Nutrition and Prevention of Growth Disorders 114 (pp. 243–255). Rome: EAAP publication.
-
Delmas PD (1993). Biochemical markers of bone turnover. Journal of Bone and Mineral Research 8: S549–S555.
-
Lepage OM, Carstanjen B and Uebelhart D (2001). Noninvasive assessment of equine bone: an update. The Veterinary Journal 161: 10–23.
-
Price JS, Jackson BF, Gray JA, Harris PA, Wright IM, Pfeiffer DU, Robins SP, Eastell R and Ricketts SW (2001). Biochemical markers of bone metabolism in growing Thoroughbreds: a longitudinal study. Research in Veterinary Science 71: 37–44.
-
Carstanjen B, Amory H, Sulon J,HarsO, Remy B, Langlois Pand Lepage OM (2005). Serum osteocalcin and CTX-MMP concentration in young exercising Thoroughbred racehorses. Journal of Veterinary Medicine Series A 52: 114–120.
-
Black A, Schoknecht PA, Ralston SL and Shapes SA (1999). Diurnal variation and age differences in biochemical markers of bone turnover in horses. Journal of Animal Science 77: 75–83.
-
Lepage OM, Marcoux M and Tremblay A (1990). Serum osteocalcin or bone Gla-protein a biochemical marker for bone metabolism in horses: differences in serum levels with age. Canadian Journal of Veterinary Research 5: 223–226.
-
Lepage OM, Eicher R, Uebelhart B and Tschudi P (1997). Influence of type and breed of horses on serum osteocalcin concentration and evaluation of the applicability of a bovine RIA and a human IRMA. American Journal of Veterinary Research 5: 48–57.
-
Price JS, Jackson BF, Eastell R, Goodship AE, Blumsohn A, Wright I, Stoneham S, Lanyon LE and Russell RGG (1995). Age related changes in biochemical markers of bone metabolism in horses. Equine Veterinary Journal 27: 201–207.
-
Robert C, Valette JP and Denoix JM (2006). Correlation between routine radiographic findings and early racing career in French Trotters. Equine Veterinary Journal Supplement 36: 473–478.
-
Hoppe F (1984). Radiological investigations of osteochondrosis dissecans in Standardbred Trotters and Swedish Warmblood horses. Equine Veterinary Journal 1: 425–429.
-
Pool RR (1993). Difficulties in definition of equine osteochondrosis; differentiation of developmental and acquired lesions. Equine Veterinary Journal Supplement 16: 5–12.
-
Jaeschke G (1975). Routine determination of free hydroxyproline in horse serum methods and normal values. Zentralblatt für Veterinarmedecin A 22: 89–101.
-
Delmas PD, Christiansen C, Mann KG and Price PA (1990). Bone Gla Protein (Osteocalcin) Assay standardization report. Journal of Bone and Mineral Research 5: 5–11.
-
Hank AM, Hoffmann WE, Sanecki RK, Schaeffer DJ and Dorner JL (1993). Quantitative determination of equine alkaline phosphatase isoenzymes in foal and adult serum. Journal of Veterinary Internal Medecine 7: 20–24.
-
Billinghurst RC, Brama PA, van Weeren PR, Knowlton MS and McIlwraith CW (2005). Evaluation of serum concentrations of biomarkers of skeletal metabolism and results of radiography as indicators of severity of osteochondrosis in foals. American Journal of Veterinary Research 65: 143–150.
-
Jackson BF, Lonnell C, Verheyen KL, Dyson P, Pfeiffer DU and Price JS (2005). Biochemical markers of bone metabolism and risk of dorsal metacarpal disease in 2-year-old Thoroughbreds. Equine veterinary Journal 37: 87–91.
-
Ducy P, Desbois CH, Boyce B, Pinero G, Story B, Dunstan C, Smith E, Bonadio J, Goldstein S, Gundberg C, Bradley A and Karsenty G (1996). Increased bone formation in osteocal-cin-deficient mice. Nature 38: 448–452.
-
Jackson BF, Blumsohn A, Goodship AE, Wilson AM and Price JS (2003). Circadian variation in biochemical markers of bone cell activity and insulin-like growth factor-I in two-year-old horses. Journal of Animal Science 11: 2804–2810.
-
May SA, Wyn-Jones G and Peremans KY (1986). Importance of oblique views in radiography of the equine limb. Equine Veterinary Journal 18(1): 7–13.
-
van Weeren PR, Sloet van Oldruitenborgh-Oosterbaan M and Barneveld A (1999). The influence of birth weight, rate of weight gain and final achieved height and sex on the development of osteochondrotic lesions in a population of genetically predisposed Warmblood foals. Equine Veterinary Journal Supplement 31: 26–30.
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Evolution of some biochemical markers of growth in relation to osteoarticular status in young horses: results of a longitudinal study in three breeds
In: Comparative Exercise PhysiologySearch for other papers by J.P. Valette in
Current site
Google Scholar
PubMed
Search for other papers by C. Robert in
Current site
Google Scholar
PubMed
Search for other papers by M.P. Toquet in
Current site
Google Scholar
PubMed
Search for other papers by J.M. Denoix in
Current site
Google Scholar
PubMed
Search for other papers by G. Fortier in
Current site
Google Scholar
PubMed
Abstract
Osteocalcin (OC), bone fraction of alkaline phosphatases (BAP) and hydroxyproline (HOP) are markers of bone cell activity. The kinetics of these markers and the analysis of their variations could be related to the osteoarticular status (OAS) of young horses. The growth of Thoroughbreds, French Trotters and Selle Franc̨ais horses was followed up to 18 months. Blood samples were taken regularly to measure OC, HOP and BAP by standardized techniques. The OAS was evaluated by radiographic examination of the limbs. Based on radiographic findings, two groups of horses were investigated, with no lesions or severely affected. Analysis of variance was used to detect the effects of age and breed, and OAS on parameters. The logarithmic model was used to determine the kinetics of the markers. A rapid decrease in marker concentrations with age and differences between breed was observed. At birth, BAP, OC and HOP concentrations were significantly higher in normal horses (1910 UI l−1, 192 ng ml−1 and 35 mg l−1, respectively) than in horses with severe lesions (1620 UI l−1, 149 ng ml−1 and 24 mg l−1, respectively). During the first 6 months, OC, HOP and BAP remained lower in severely affected horses.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 71 | 36 | 6 |
| PDF Views & Downloads | 50 | 25 | 6 |
