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Investigating therapeutic efficacy of Lactobacillus salivarius GZLC1 on colitis by examining its effects on intestinal fungi, antioxidant capacity, and inflammatory response in mice

Beneficial Microbes
著者:
J. Ma Bijie Academy of Agricultural Sciences, Bijie 551799, China P.R

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D. Wu Bijie Academy of Agricultural Sciences, Bijie 551799, China P.R

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M. Liu School of Basic Medicine, Hubei University of Arts and Science, Xiangyang 441053, China P.R

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C. Xu College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China P.R

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Q. He College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China P.R

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M. Wang Bijie Academy of Agricultural Sciences, Bijie 551799, China P.R

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F.A. Kiani Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan

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M.S. Rafiq National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China P.R

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K. Mehmood Faculty of Veterinary and Animal Sciences, The University of Bahawalpur, 63100, Pakistan

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D. Fouad Department of Zoology, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia

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F.S. Ataya Department of Biochemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia

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K. Li College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China P.R

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Abstract

We investigated the therapeutic potential of Lactobacillus salivarius in colitis mice, and the mice were randomly allocated into three groups with each consisting of 10 mice (n = 10): a control group (CSG), a DSS-induced colitis model group (DSG), and a L. salivarius intervention group (LSG). The intervention group received daily oral administration of L. salivarius for seven consecutive days. Compared to the DSS model group, mice receiving L. salivarius exhibited significantly reduced weight loss, lower DAI scores, lessened colon shortening, and improved histopathological profiles, indicating a substantial reduction in inflammatory damage. Additionally, ITS sequencing revealed that L. salivarius significantly influenced the composition of the intestinal fungal community, decreasing the abundance of pathogenic fungi, such as Candida species, by approximately 40%, and restoring fungal homeostasis by reducing the Basidiomycota/Ascomycota ratio. Moreover, L. salivarius effectively alleviated DSS-induced oxidative stress by lowering serum MDA levels while enhancing the activity of SOD and GSH-Px. Furthermore, the probiotic intervention resulted in 30-40% reduction in pro-inflammatory factors (TNF-α, IL-6, and IL-1β) and an increase in the anti-inflammatory factor IL-10 levels, suggesting a pronounced anti-inflammatory effect. In conclusion, L. salivarius exerts significant protective effects against DSS-induced colitis by modulating the gut fungal community, mitigating oxidative stress, and suppressing inflammatory responses. This study is novel in that few probiotic studies have investigated the impact of L. salivarius on gut fungi in DSS-induced colitis. These findings highlight its potential as a therapeutic candidate for managing inflammatory bowel disease.

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