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Butyrate modulating effects on pro-inflammatory pathways in human intestinal epithelial cells

In: Beneficial Microbes
Authors:
A. Elce Dipartimento di Scienze Umanistiche, Università Telematica Pegaso, Piazza Trieste e Trento, 48, 80132 Naples, Italy.
CEINGE-Biotecnologie avanzate, Via Gaetano Salvatore 486, Naples 80145, Italy.

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F. Amato CEINGE-Biotecnologie avanzate, Via Gaetano Salvatore 486, Naples 80145, Italy.
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.

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F. Zarrilli Dipartimento di Bioscienze e Territorio, Università del Molise, Contrada Fonte Lappone, 86090 Pesche, Isernia, Italy.
CEINGE-Biotecnologie avanzate, Via Gaetano Salvatore 486, Naples 80145, Italy.

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A. Calignano Dipartimento di Farmacia, Università di Napoli Federico II, Montesano, Via Domenico Montesano 49, 80131 Naples, Italy.

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R. Troncone Dipartimento di Scienze Mediche Traslazionali, Sezione di Pediatria, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
European Laboratory for the Investigation of Food-Induced Diseases, Via S. Pansini 5, 80131 Naples, Italy.

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G. Castaldo CEINGE-Biotecnologie avanzate, Via Gaetano Salvatore 486, Naples 80145, Italy.
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.

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R.B. Canani Dipartimento di Scienze Mediche Traslazionali, Sezione di Pediatria, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
European Laboratory for the Investigation of Food-Induced Diseases, Via S. Pansini 5, 80131 Naples, Italy.

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Butyrate acts as energy source for intestinal epithelial cells and as key mediator of several immune processes, modulating gene expression mainly through histone deacetylation inhibition. Thanks to these effects, butyrate has been proposed for the treatment of many intestinal diseases. Aim of this study was to investigate the effect of butyrate on the expression of a large series of target genes encoding proteins involved in pro-inflammatory pathways. We performed quantitative real-time-PCR analysis of the expression of 86 genes encoding proteins bearing to pro-inflammatory pathways, before and after butyrate exposure, in primary epithelial cells derived from human small intestine and colon. Butyrate significantly down-regulated the expression of genes involved in inflammatory response, among which nuclear factor kappa beta, interferon-gamma, Toll like 2 receptor and tumour necrosis factor-alpha. Further confirmations of these data, including studies at protein level, would support the use of butyrate as effective therapeutic strategy in intestinal inflammatory disorders.

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