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Lactobacillus plantarum L9 but not Lactobacillus acidophilus LA reduces tumour necrosis factor induced bacterial translocation in Caco-2 cells
in Beneficial MicrobesDepartment of Travel Health, Nanjing Entry-Exit Inspection and Quarantine Bureau, Nanjing, Jiangsu, 211106, China P.R.
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Translocation of bacteria across the intestinal barrier is important in the pathogenesis of systemic sepsis and multiple organ dysfunction syndromes. Inflammatory cytokines increase paracellular permeability that allows increased luminal bacteria to translocate across mucosal epithelium and further deteriorate the gut barrier. In order to reduce this risk, the prophylactic use of probiotics has been recently addressed. In this paper, we investigate the protective role toward tumour necrosis factor (TNF)-α induced non-pathogenic Escherichia coli translocation across Caco-2 monolayers of Lactobacillus strains. According to our experimental data, Lactobacillus plantarum L9 and Lactobacillus acidophilus LA have good capacities to adhere to Caco-2 cells. Addition of L. plantarum L9 and L. acidophilus LA to the enterocyte monolayer surface result in significant inhibition of E. coli adhesion and cell internalisation. However, L. plantarum L9 and L. acidophilus LA did not inhibit the growth of the non-pathogenic E. coli B5 after 24 h incubation. Exposure to TNF-α for 6 h caused a dramatic increase in E. coli B5 translocation across Caco-2 cells, which was uncoupled from increases in paracellular permeability. Pretreatment with L. plantarum L9 prevent TNF-α induced transcellular bacterial translocation and IL-8 production in Caco-2 cells. L. plantarum L9 also did not affect the integrity of the monolayers, as indicated by lactate dehydrogenase release, horseradish peroxidase permeability, and transepithelial electrical resistance. L. plantarum L9 showed the potential to protect enterocytes from an acute inflammatory response and therefore could be good potential prophylactic agents in counteracting bacterial translocation.
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-
Alexander, J.W., Boyce, S.T., Babcock, G.F., Gianotti, L., Peck, M.D., Dunn, D.L., Pyles, T., Childress, C.P. and Ash, S.K., 1990. The process of microbial translocation. Annals of Surgery 212: 496-510.
'The process of microbial translocation ' () 212 Annals of Surgery : 496 -510.
-
Anderssen, E.L., Diep, D.B., Nes, I.F., Eijsink, V.G. and Nissen-Meyer, J., 1998. Antagonistic activity of Lactobacillus plantarum C11: two new two-peptide bacteriocins, plantaricins EF and JK, and the induction factor plantaricin A. Applied and Environmental Microbiology 64: 2269-2272.
'Antagonistic activity of Lactobacillus plantarum C11: two new two-peptide bacteriocins, plantaricins EF and JK, and the induction factor plantaricin A ' () 64 Applied and Environmental Microbiology : 2269 -2272.
-
Artis, D., 2008. Epithelial-cell recognition of commensal bacteria and maintenance of immune homeostasis in the gut. Nature Reviews Immunology 8: 411-420.
'Epithelial-cell recognition of commensal bacteria and maintenance of immune homeostasis in the gut ' () 8 Nature Reviews Immunology : 411 -420.
-
Bauer, T.M., Fernández, J., Navasa, M., Vila, J. and Rodés, J., 2002. Failure of Lactobacillus spp. to prevent bacterial translocation in a rat model of experimental cirrhosis. Journal of Hepatology 36: 501-506.
'Failure of Lactobacillus spp. to prevent bacterial translocation in a rat model of experimental cirrhosis ' () 36 Journal of Hepatology : 501 -506.
-
Berg, R.D., 1995. Bacterial translocation from the gastrointestinal tract. Trends in Microbiology 3: 149-154.
'Bacterial translocation from the gastrointestinal tract ' () 3 Trends in Microbiology : 149 -154.
-
Berkes, J., Viswanathan, V.K., Savkovic, S.D. and Hecht, G., 2003. Intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation. Gut 52: 439-451.
'Intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation ' () 52 Gut : 439 -451.
-
Bernet, M.F., Brassart, D., Neeser, J.R. and Servin, A.L., 1993. Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions. Applied and Environmental Microbiology 59: 4121-4128.
'Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions ' () 59 Applied and Environmental Microbiology : 4121 -4128.
-
Bernet, M.F., Brassart, D., Neeser, J.R. and Servin, A.L., 1994. Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. Gut 35: 483-489.
'Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria ' () 35 Gut : 483 -489.
-
Borruel, N., Casellas, F., Antolin, M., Llopis, M., Carol, M., Espiin, E., Naval, J., Guarner, F. and Malagelada, J.R., 2003. Effects of nonpathogenic bacteria on cytokine secretion by human intestinal mucosa. American Journal of Gastroenterology 98: 865-870.
'Effects of nonpathogenic bacteria on cytokine secretion by human intestinal mucosa ' () 98 American Journal of Gastroenterology : 865 -870.
-
Clark, E., Hoare, C., Tanianis-Hughes, J., Carlson, G.L. and Warhurst, G., 2005. Interferon gamma induces translocation of commensal Escherichia coli across gut epithelial cells via a lipid raft-mediated process. Gastroenterology 128: 1258-1267.
'Interferon gamma induces translocation of commensal Escherichia coli across gut epithelial cells via a lipid raft-mediated process ' () 128 Gastroenterology : 1258 -1267.
-
Clark, E.C., Patel, S.D., Chadwick, P.R., Warhurst, G., Curry, A. and Carlson, G.L., 2003. Glutamine deprivation facilitates tumour necrosis factor induced bacterial translocation in Caco-2 cells by depletion of enterocyte fuel substrate. Gut 52: 224-230.
'Glutamine deprivation facilitates tumour necrosis factor induced bacterial translocation in Caco-2 cells by depletion of enterocyte fuel substrate ' () 52 Gut : 224 -230.
-
Daig, R., Andus, T., Aschenbrenner, E., Falk, W., Schölmerich, J. and Gross, V., 1996. Increased interleukin 8 expression in the colon mucosa of patients with inflammatory bowel disease. Gut 38: 216-222.
'Increased interleukin 8 expression in the colon mucosa of patients with inflammatory bowel disease ' () 38 Gut : 216 -222.
-
Fayol-Messaoudi, D., Berger, C.N., Coconnier-Polter, M.H., Liévin-Le Moal, V. and Servin, A.L., 2005. pH-, Lactic acid-, and non-lactic acid-dependent activities of probiotic lactobacilli against Salmonella enterica serovar Typhimurium. Applied and Environmental Microbiology 71: 6008-6013.
'pH-, Lactic acid-, and non-lactic acid-dependent activities of probiotic lactobacilli against Salmonella enterica serovar Typhimurium ' () 71 Applied and Environmental Microbiology : 6008 -6013.
-
Grotz, M.R., Deitch, E.A., Ding, J., Xu, D., Huang, Q. and Regel, G., 1999. Intestinal cytokine response after gut ischemia: role of gut barrier failure. Annals of Surgery 229: 478-486.
'Intestinal cytokine response after gut ischemia: role of gut barrier failure ' () 229 Annals of Surgery : 478 -486.
-
Lu, L. and Walker, W.A., 2001. Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium. American Journal of Clinical Nutrition 73: 1124S-1130S.
'Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium ' () 73 American Journal of Clinical Nutrition : 1124S -1130S.
-
Ma, D., Forsythe, P. and Bienenstock, J., 2004. Live Lactobacillus reuteri is essential for the inhibitory effect on tumor necrosis factor alpha-induced interleukin-8 expression. Infection and Immunity 72: 5308-5314.
'Live Lactobacillus reuteri is essential for the inhibitory effect on tumor necrosis factor alpha-induced interleukin-8 expression ' () 72 Infection and Immunity : 5308 -5314.
-
MacFie, J., O’Boyle, C., Mitchell, C.J., Buckley, P.M., Johnstone, D. and Sudworth, P., 1999. Gut origin of sepsis: a prospective study investigating associations between bacterial translocation, gastric microflora, and septic morbidity. Gut 45: 223-228.
'Gut origin of sepsis: a prospective study investigating associations between bacterial translocation, gastric microflora, and septic morbidity ' () 45 Gut : 223 -228.
-
Macutkiewicz, C., Carlson, G., Clark, E., Dobrindt, U., Roberts, I. and Warhurst, G., 2008. Characterisation of Escherichia coli strains involved in transcytosis across gut epithelial cells exposed to metabolic and inflammatory stress. Microbes and Infection 10: 424-431.
'Characterisation of Escherichia coli strains involved in transcytosis across gut epithelial cells exposed to metabolic and inflammatory stress ' () 10 Microbes and Infection : 424 -431.
-
Mangell, P., Lennernäs, P., Wang, M., Olsson, C., Ahrné, S., Molin, G., Thorlacius, H. and Jeppsson, B., 2006. Adhesive capability of Lactobacillus plantarum 299V is immprotant for preventing bacterial translocation in endotoxemic rats. APMIS 114: 611-618.
'Adhesive capability of Lactobacillus plantarum 299V is immprotant for preventing bacterial translocation in endotoxemic rats ' () 114 APMIS : 611 -618.
-
Mattila-Sandholm, T., Mättö, J. and Saarela, M., 1999. Lactic acid bacteria with health claims – interactions and interference with gastrointestinal flora. International Dairy Journal 9: 25-35.
'Lactic acid bacteria with health claims – interactions and interference with gastrointestinal flora ' () 9 International Dairy Journal : 25 -35.
-
McGuckin, M.A., Eri, R., Simms, L.A., Florin, T.H. and Radford-Smith, G., 2009. Intestinal barrier dysfunction in inflammatory bowel diseases. Inflammatory Bowel Diseases 15: 100-113.
'Intestinal barrier dysfunction in inflammatory bowel diseases ' () 15 Inflammatory Bowel Diseases : 100 -113.
-
Nazli, A., Wang, A., Steen, O., Prescott, D., Lu, J., Perdue, M.H., Söderholm, J.D., Sherman, P.M. and McKay, D.M., 2006. Enterocyte cytoskeleton changes are crucial for enhanced translocation of nonpathogenic Escherichia coli across metabolically stressed gut epithelia. Infection and Immunity 74: 192-201.
'Enterocyte cytoskeleton changes are crucial for enhanced translocation of nonpathogenic Escherichia coli across metabolically stressed gut epithelia ' () 74 Infection and Immunity : 192 -201.
-
Peitzman, A.B., Udekwu, A.O., Ochoa, J. and Smith, S., 1991. Bacterial translocation in trauma patients. Journal of Trauma 31: 1083-1086.
'Bacterial translocation in trauma patients ' () 31 Journal of Trauma : 1083 -1086.
-
Sartor, R.B., 2004. Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases: antibiotics, probiotics and prebiotics. Gastroenterology 126: 1620-1633.
'Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases: antibiotics, probiotics and prebiotics ' () 126 Gastroenterology : 1620 -1633.
-
Schillinger, U. and Lücke, F.K., 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Applied and Environmental Microbiology 55: 1901-1906.
'Antibacterial activity of Lactobacillus sake isolated from meat ' () 55 Applied and Environmental Microbiology : 1901 -1906.
-
Sedman, P.C., MacFie, J., Sagar, P., Mitchell, C.J., May, J., Mancey-Jones, B. and Johnstone, D., 1994. The prevalence of gut translocation in humans. Gastroenterology 107: 643-649.
'The prevalence of gut translocation in humans ' () 107 Gastroenterology : 643 -649.
-
Servin, A.L., 2004. Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiology Reviews 28: 405-440.
'Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens ' () 28 FEMS Microbiology Reviews : 405 -440.
-
Seydel, K.B., Li, E., Swanson, P.E. and Stanley Jr., S.L., 1997. Human intestinal epithelial cells produce proinflammatory cytokines in response to infection in a SCID mouse-human intestinal xenograft model of amebiasis. Infection and Immunity 65: 1631-1639.
'Human intestinal epithelial cells produce proinflammatory cytokines in response to infection in a SCID mouse-human intestinal xenograft model of amebiasis ' () 65 Infection and Immunity : 1631 -1639.
-
Sherman, P.M., Ossa, J.C. and Johnson-Henry, K., 2009. Unraveling mechanisms of action of probiotics. Nutrition in Clinical Practice 24: 10-14.
'Unraveling mechanisms of action of probiotics ' () 24 Nutrition in Clinical Practice : 10 -14.
-
Stecher, B. and Hardt, W.D., 2008. The role of microbiota in infectious disease. Trends in Microbiology 16: 107-114.
'The role of microbiota in infectious disease ' () 16 Trends in Microbiology : 107 -114.
-
Swank, G.M. and Deitch, E.A., 1996. Role of the gut in multiple organ failure: bacterial translocation and permeability changes. World Journal of Surgery 20: 411-417.
'Role of the gut in multiple organ failure: bacterial translocation and permeability changes ' () 20 World Journal of Surgery : 411 -417.
-
Todoriki, K., Mukai, T., Sato, S. and Toba, T., 2001. Inhibition of adhesion of food-borne pathogens to Caco-2 cells by Lactobacillus strains. Journal of Applied Microbiology 91: 154-159.
'Inhibition of adhesion of food-borne pathogens to Caco-2 cells by Lactobacillus strains ' () 91 Journal of Applied Microbiology : 154 -159.
-
Wang, B., Li, J., Li, Q., Zhang, H. and Li, N., 2009. Isolation of adhesive strains and evaluation of the colonization and immune response by Lactobacillus plantarum L2 in the rat gastrointestinal tract. International Journal of Food Microbiology 132: 59-66.
'Isolation of adhesive strains and evaluation of the colonization and immune response by Lactobacillus plantarum L2 in the rat gastrointestinal tract ' () 132 International Journal of Food Microbiology : 59 -66.
-
Wang, B., Li, J., Chen, J., Huang, Q., Li, N. and Li, J., 2010. Effect of live Lactobacillus plantarum L2 on TNF-α-induced MCP-1 production in Caco-2 cell. International Journal of Food Microbiology 142: 237-241.
'Effect of live Lactobacillus plantarum L2 on TNF-α-induced MCP-1 production in Caco-2 cell ' () 142 International Journal of Food Microbiology : 237 -241.
-
Wells, C.L., Van de Westerlo, E.M., Jechorek, R.P., Haines, H.M. and Erlandsen, S.L., 1998. Cytochalasin-induced actin disruption of polarized enterocytes can augment internalization of bacteria. Infection and Immunity 66: 2410-2419.
'Cytochalasin-induced actin disruption of polarized enterocytes can augment internalization of bacteria ' () 66 Infection and Immunity : 2410 -2419.
-
Ye, D., Ma, I. and Ma, T.Y., 2006. Molecular mechanism of tumor necrosis factor-alpha modulation of intestinal epithelial tight junction barrier. American Journal of Physiology Gastrointestinal and Liver Physiology 290: G496-504.
'Molecular mechanism of tumor necrosis factor-alpha modulation of intestinal epithelial tight junction barrier ' () 290 American Journal of Physiology Gastrointestinal and Liver Physiology : G496 -504.
-
Youakim, A. and Ahdieh, M., 1999. Interferon-gamma decreases barrier function in T84 cells by reducing ZO-1 levels and disrupting apical actin. American Journal of Physiology 276: G1279-1288.
'Interferon-gamma decreases barrier function in T84 cells by reducing ZO-1 levels and disrupting apical actin ' () 276 American Journal of Physiology : G1279 -1288.
-
Zallen, G., Moore, E.E., Johnson, J.L., Tamura, D.Y., Aiboshi, J., Biffl, W.L. and Silliman, C.C., 1999. Circulating postinjury neutrophils are primed for the release of proinflammatory cytokines. Journal of Trauma 46: 42-48.
'Circulating postinjury neutrophils are primed for the release of proinflammatory cytokines ' () 46 Journal of Trauma : 42 -48.
-
Zareie, M., Johnson-Henry, K., Jury, J., Yang, P.C., Ngan, B.Y., McKay, D.M., Soderholm, J.D., Perdue, M.H. and Sherman, P.M., 2006. Probiotics prevent bacterial translocation and improve intestinal barrier function in rats following chronic psychological stress. Gut 55: 1553-1560.
'Probiotics prevent bacterial translocation and improve intestinal barrier function in rats following chronic psychological stress ' () 55 Gut : 1553 -1560.
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Lactobacillus plantarum L9 but not Lactobacillus acidophilus LA reduces tumour necrosis factor induced bacterial translocation in Caco-2 cells
in Beneficial MicrobesDepartment of Travel Health, Nanjing Entry-Exit Inspection and Quarantine Bureau, Nanjing, Jiangsu, 211106, China P.R.
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PubMed
Translocation of bacteria across the intestinal barrier is important in the pathogenesis of systemic sepsis and multiple organ dysfunction syndromes. Inflammatory cytokines increase paracellular permeability that allows increased luminal bacteria to translocate across mucosal epithelium and further deteriorate the gut barrier. In order to reduce this risk, the prophylactic use of probiotics has been recently addressed. In this paper, we investigate the protective role toward tumour necrosis factor (TNF)-α induced non-pathogenic Escherichia coli translocation across Caco-2 monolayers of Lactobacillus strains. According to our experimental data, Lactobacillus plantarum L9 and Lactobacillus acidophilus LA have good capacities to adhere to Caco-2 cells. Addition of L. plantarum L9 and L. acidophilus LA to the enterocyte monolayer surface result in significant inhibition of E. coli adhesion and cell internalisation. However, L. plantarum L9 and L. acidophilus LA did not inhibit the growth of the non-pathogenic E. coli B5 after 24 h incubation. Exposure to TNF-α for 6 h caused a dramatic increase in E. coli B5 translocation across Caco-2 cells, which was uncoupled from increases in paracellular permeability. Pretreatment with L. plantarum L9 prevent TNF-α induced transcellular bacterial translocation and IL-8 production in Caco-2 cells. L. plantarum L9 also did not affect the integrity of the monolayers, as indicated by lactate dehydrogenase release, horseradish peroxidase permeability, and transepithelial electrical resistance. L. plantarum L9 showed the potential to protect enterocytes from an acute inflammatory response and therefore could be good potential prophylactic agents in counteracting bacterial translocation.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 557 | 130 | 30 |
| Gesamttextansichten | 24 | 0 | 0 |
| PDF-Downloads | 12 | 0 | 0 |
