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Alleviation of enterotoxigenic Escherichia coli challenge by recombinant Lactobacillus plantarum expressing a FaeG- and DC-targeting peptide fusion protein
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FaeG is the major subunit of K88 fimbriae. These cell surface attachments are considered to be the major virulence factor of enterotoxigenic Escherichia coli (ETEC), which causes diarrhoea in piglets. The use of dendritic cell-targeting peptide (DCpep) has been demonstrated to be an effective approach to enhance the immunity of vaccines. Lactobacillus plantarum is an attractive candidate for oral vaccination owing to its beneficial effects and safety. In this study, L. plantarum was employed to deliver a FaeG-DCpep fusion antigen, and the immune response in mice was evaluated. The synthesis of FaeG-DCpep dramatically increased the adhesion of recombinant L. plantarum (RLP) to IPEC-J2 cell surfaces, resulting in direct competition between L. plantarum and ETEC during adhesion assays. Significantly higher levels of body weight gain, sera immunoglobulin G and intestinal immunoglobulin A were observed in BALB/c mice immunised with RLP. In addition, the number of CD19+ B cells and CD11c+DC cells and the expression levels of several cytokines in the spleen and lymph nodes increased significantly compared to non-immunised mice. The oral administration of RLP also alleviated the symptoms of ETEC challenge, as shown by haematoxylin-eosin staining, indicating that RLP may be an efficient vaccine candidate.
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-
Banchereau, J. and Steinman, R.M., 1998. Dendritic cells and the control of immunity. Nature 392: 245-252.
'Dendritic cells and the control of immunity ' () 392 Nature : 245 -252.
-
Borre, Y.E., Moloney, R.D., Clarke, G., Dinan, T.G. and Cryan, J.F., 2014. The impact of microbiota on brain and behavior: mechanisms and therapeutic potential. Advances in Experimental Medicine and Biology 817: 373-403.
'The impact of microbiota on brain and behavior: mechanisms and therapeutic potential ' () 817 Advances in Experimental Medicine and Biology : 373 -403.
-
Chowdhury, M.Y., Li, R., Kim, J.H., Park, M.E., Kim, T.H., Pathinayake, P., Weeratunga, P., Song, M.K., Son, H.Y., Hong, S.P., Sung, M.H., Lee, J.S. and Kim, C.J., 2014. Mucosal vaccination with recombinant Lactobacillus casei- displayed CTA1-conjugated consensus matrix protein-2 (sM2) induces broad protection against divergent influenza subtypes in BALB/c mice. PLoS ONE 9: e94051.
'Mucosal vaccination with recombinant Lactobacillus casei- displayed CTA1-conjugated consensus matrix protein-2 (sM2) induces broad protection against divergent influenza subtypes in BALB/c mice ' () 9 PLoS ONE : e94051.
-
Curiel, T.J., Morris, C., Brumlik, M., Landry, S.J., Finstad, K., Nelson, A., Joshi, V., Hawkins, C., Alarez, X., Lackner, A. and Mohamadzadeh, M., 2004. Peptides identified through phage display direct immunogenic antigen to dendritic cells. Journal of Immunology 172: 7425-7431.
'Peptides identified through phage display direct immunogenic antigen to dendritic cells ' () 172 Journal of Immunology : 7425 -7431.
-
Dieye, Y., Usai, S., Clier, F., Gruss, A. and Piard, J.C., 2001. Design of a protein-targeting system for lactic acid bacteria. Journal of Bacteriology 183: 4157-4166.
'Design of a protein-targeting system for lactic acid bacteria ' () 183 Journal of Bacteriology : 4157 -4166.
-
Garcia-Fruitos, E., 2012. Lactic acid bacteria: a promising alternative for recombinant protein production. Microbial Cell Factories 11: 157.
'Lactic acid bacteria: a promising alternative for recombinant protein production ' () 11 Microbial Cell Factories : 157.
-
Gilliland, S.E., 1990. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiology Reviews 7: 175-188.
'Health and nutritional benefits from lactic acid bacteria ' () 7 FEMS Microbiology Reviews : 175 -188.
-
Grangette, C., Muller-Alouf, H., Geoffroy, M., Goudercourt, D., Turneer, M. and Mercenier, A., 2002. Protection against tetanus toxin after intragastric administration of two recombinant lactic acid bacteria: impact of strain viability and in vivo persistence. Vaccine 20: 3304-3309.
'Protection against tetanus toxin after intragastric administration of two recombinant lactic acid bacteria: impact of strain viability and in vivo persistence ' () 20 Vaccine : 3304 -3309.
-
Hawiger, D., Inaba, K., Dorsett, Y., Guo, M., Mahnke, K., Rivera, M., Ravetch, J.V., Steinman, R.M. and Nussenzweig, M.C., 2001. Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo. Journal of Experimental Medicine 194: 769-779.
'Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo ' () 194 Journal of Experimental Medicine : 769 -779.
-
He, L.Z., Ramakrishna, V., Connolly, J.E., Wang, X.T., Smith, P.A., Jones, C.L., Valkova-Valchanova, M., Arunakumari, A., Treml, J.F., Goldstein, J., Wallace, P.K., Keler, T. and Endres, M.J., 2004. A novel human cancer vaccine elicits cellular responses to the tumor-associated antigen, human chorionic gonadotropin beta. Clinical Cancer Research 10: 1920-1927.
'A novel human cancer vaccine elicits cellular responses to the tumor-associated antigen, human chorionic gonadotropin beta ' () 10 Clinical Cancer Research : 1920 -1927.
-
Hesse, C., Ginter, W., Forg, T., Mayer, C.T., Baru, A.M., Arnold-Schrauf, C., Unger, W.W., Kalay, H., Van Kooyk, Y., Berod, L. and Sparwasser, T., 2013. In vivo targeting of human DC-SIGN drastically enhances CD8(+) T-cell-mediated protective immunity. European Journal of Immunology 43: 2543-2553.
'In vivo targeting of human DC-SIGN drastically enhances CD8(+) T-cell-mediated protective immunity ' () 43 European Journal of Immunology : 2543 -2553.
-
Hiramatsu, Y., Hosono, A., Konno, T., Nakanishi, Y., Muto, M., Suyama, A., Hachimura, S., Sato, R., Takahashi, K. and Kaminogawa, S., 2011. Orally administered Bifidobacterium triggers immune responses following capture by CD11c(+) cells in Peyer’s patches and cecal patches. Cytotechnology 63: 307-317.
'Orally administered Bifidobacterium triggers immune responses following capture by CD11c(+) cells in Peyer’s patches and cecal patches ' () 63 Cytotechnology : 307 -317.
-
Hirayama, K. and Rafter, J., 1999. The role of lactic acid bacteria in colon cancer prevention: mechanistic considerations. Antonie Van Leeuwenhoek 76: 391-394.
'The role of lactic acid bacteria in colon cancer prevention: mechanistic considerations ' () 76 Antonie Van Leeuwenhoek : 391 -394.
-
Hosoya, S., Villena, J., Shimazu, T., Tohno, M., Fujie, H., Chiba, E., Shimosato, T., Aso, H., Suda, Y., Kawai, Y., Saito, T., Alvarez, S., Ikegami, S., Itoh, H. and Kitazawa, H., 2011. Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells. Veterinary Research 42: 111.
'Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells ' () 42 Veterinary Research : 111.
-
Hu, C.X., Xu, Z.R., Li, W.F., Niu, D., Lu, P. and Fu, L.L., 2009. Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice. Biotechnology Letters 31: 991-997.
'Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice ' () 31 Biotechnology Letters : 991 -997.
-
Jiang, Y., Hu, J., Guo, Y., Yang, W., Ye, L., Shi, C., Liu, Y., Yang, G. and Wang, C., 2015. Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC-targeting peptide fusion protein. Journal of Biotechnology 216: 82-89.
'Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC-targeting peptide fusion protein ' () 216 Journal of Biotechnology : 82 -89.
-
Jiang, Y., Yang, G., Meng, F., Yang, W., Hu, J., Ye, L., Shi, C. and Wang, C., 2016. Immunological mechanisms involved in probiotic-mediated protection against Citrobacter rodentium-induced colitis. Beneficial Microbes 7: 397-407.
'Immunological mechanisms involved in probiotic-mediated protection against Citrobacter rodentium-induced colitis ' () 7 Beneficial Microbes : 397 -407.
-
Kazemi, R., Akhavian, A., Amani, J., Salimian, J., Motamedi, M.J., Mousavi, A., Jafari, M. and Salmanian, A.H., 2016. Immunogenic properties of trivalent recombinant protein composed of B-subunits of LT, STX-2, and CT toxins. Microbes and Infection 18: 421-429.
'Immunogenic properties of trivalent recombinant protein composed of B-subunits of LT, STX-2, and CT toxins ' () 18 Microbes and Infection : 421 -429.
-
Klechevsky, E., Flamar, A.L., Cao, Y., Blanck, J.P., Liu, M., O’Bar, A., Agouna-Deciat, O., Klucar, P., Thompson-Snipes, L., Zurawski, S., Reiter, Y., Palucka, A.K., Zurawski, G. and Banchereau, J., 2010. Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR. Blood 116: 1685-1697.
'Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR ' () 116 Blood : 1685 -1697.
-
Kolotilin, I., Kaldis, A., Devriendt, B., Joensuu, J., Cox, E. and Menassa, R., 2012. Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco. PLoS ONE 7: e42405.
'Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco ' () 7 PLoS ONE : e42405.
-
Lee do, K., Jang, S., Baek, E.H., Kim, M.J., Lee, K.S., Shin, H.S., Chung, M.J., Kim, J.E., Lee, K.O. and Ha, N.J., 2009. Lactic acid bacteria affect serum cholesterol levels, harmful fecal enzyme activity, and fecal water content. Lipids Health Disease 8: 21.
'Lactic acid bacteria affect serum cholesterol levels, harmful fecal enzyme activity, and fecal water content ' () 8 Lipids Health Disease : 21.
-
Lee, J.S., Awji, E.G. Lee, S.J., Tassew, D.D., Park, Y.B., Park, K.S., Kim, M.K., Kim, B. and Park, S.C., 2012. Effect of Lactobacillus plantarum CJLP243 on the growth performance and cytokine response of weaning pigs challenged with enterotoxigenic Escherichia coli. Journal of Animal Science 90: 3709-3717.
'Effect of Lactobacillus plantarum CJLP243 on the growth performance and cytokine response of weaning pigs challenged with enterotoxigenic Escherichia coli ' () 90 Journal of Animal Science : 3709 -3717.
-
Li, D., Romain, G., Flamar, A.L., Duluc, D., Dullaers, M., Li, X.H., Zurawski, S., Bosquet, N., Palucka, A.K., Le Grand, R., O’Garra, A., Zurawski, G., Banchereau, J. and Oh, S., 2012. Targeting self- and foreign antigens to dendritic cells via DC-ASGPR generates IL-10-producing suppressive CD4+ T cells. Journal of Experimental Medicine 209: 109-121.
'Targeting self- and foreign antigens to dendritic cells via DC-ASGPR generates IL-10-producing suppressive CD4+ T cells ' () 209 Journal of Experimental Medicine : 109 -121.
-
Lillie, R.D., 1965. Histopathologic technic and practical histochemistry. McGraw-Hill, New York, NY, USA, 715 pp.
'Histopathologic technic and practical histochemistry ', () 715.
-
Mariman, R., Tielen, F., Koning, F. and Nagelkerken, L., 2015. The probiotic mixture VSL#3 has differential effects on intestinal immune parameters in healthy female BALB/c and C57BL/6 mice. Journal of Nutrition 145: 1354-1361.
'The probiotic mixture VSL#3 has differential effects on intestinal immune parameters in healthy female BALB/c and C57BL/6 mice ' () 145 Journal of Nutrition : 1354 -1361.
-
Melkebeek, V., Goddeeris, B.M. and Cox, E., 2013. ETEC vaccination in pigs. Veterinary Immunology and Immunopathology 152: 37-42.
'ETEC vaccination in pigs ' () 152 Veterinary Immunology and Immunopathology : 37 -42.
-
Mohamadzadeh, M., Duong, T., Sandwick, S.J., Hoover, T. and Klaenhammer, T.R., 2009. Dendritic cell targeting of Bacillus anthracis protective antigen expressed by Lactobacillus acidophilus protects mice from lethal challenge. Proceedings of the National Academy of Sciences of the USA 106: 4331-4336.
'Dendritic cell targeting of Bacillus anthracis protective antigen expressed by Lactobacillus acidophilus protects mice from lethal challenge ' () 106 Proceedings of the National Academy of Sciences of the USA : 4331 -4336.
-
Morello, E., Bermudez-Humaran, L.G., Llull, D., Sole, V., Miraglio, N., Langella, P. and Poquet, I., 2008. Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion. Journal of Molecular Microbiology and Biotechnology 14: 48-58.
'Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion ' () 14 Journal of Molecular Microbiology and Biotechnology : 48 -58.
-
Nagy, B. and Fekete, P.Z., 1999. Enterotoxigenic Escherichia coli (ETEC) in farm animals. Veterinary Research 30: 259-284.
'Enterotoxigenic Escherichia coli (ETEC) in farm animals ' () 30 Veterinary Research : 259 -284.
-
Ni, L., Gayet, I., Zurawski, S., Duluc, D., Flamar, A.L., Li, X.H., O’Bar, A., Clayton, S., Palucka, A.K., Zurawski, G., Banchereau, J. and Oh, S., 2010. Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses. Journal of Immunology 185: 3504-3513.
'Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses ' () 185 Journal of Immunology : 3504 -3513.
-
Nolte, M.A., Hoen, E.N., Van Stijn, A., Kraal, G. and Mebius, R.E., 2000. Isolation of the intact white pulp. Quantitative and qualitative analysis of the cellular composition of the splenic compartments. European Journal of Immunology 30: 626-634.
'Isolation of the intact white pulp ' () 30 European Journal of Immunology : 626 -634.
-
Nouaille, S., Ribeiro, L.A., Miyoshi, A., Pontes, D., Le Loir, Y., Oliveira, S.C., Langella, P. and Azevedo, V., 2003. Heterologous protein production and delivery systems for Lactococcus lactis. Genetics and Molecular Research 2: 102-111.
'Heterologous protein production and delivery systems for Lactococcus lactis ' () 2 Genetics and Molecular Research : 102 -111.
-
Perdigon, G., Fuller, R. and Raya, R., 2001. Lactic acid bacteria and their effect on the immune system. Current Issues in Intestinal Microbiology 2: 27-42.
'Lactic acid bacteria and their effect on the immune system ' () 2 Current Issues in Intestinal Microbiology : 27 -42.
-
Perdigon, G., Maldonado Galdeano, C., Valdez, J.C. and Medici, M., 2002. Interaction of lactic acid bacteria with the gut immune system. European Journal of Clinical Nutrition 56, Suppl. 4: S21-26.
'Interaction of lactic acid bacteria with the gut immune system ' () 4 European Journal of Clinical Nutrition : S21 -26.
-
Rafter, J., 2002. Lactic acid bacteria and cancer: mechanistic perspective. British Journal of Nutrition 88, Suppl. 1: S89-94.
'Lactic acid bacteria and cancer: mechanistic perspective ' () 1 British Journal of Nutrition : S89 -94.
-
Rausch, D., Ruan, X., Nandre, R., Duan, Q., Hashish, E., Casey, T.A. and Zhang, W., in press. Antibodies derived from a toxoid MEFA (multiepitope fusion antigen) show neutralizing activities against heat-labile toxin (LT), heat-stable toxins (STa, STb), and Shiga toxin 2e (Stx2e) of porcine enterotoxigenic Escherichia coli (ETEC). Veterinary Microbiology. DOI: http://dx.doi.org/10.1016/j.vetmic.2016.02.002.
-
Rolny, I.S., Tiscornia, I., Racedo, S.M., Perez, P.F. and Bollati-Fogolin, M., 2016. Lactobacillus delbrueckii subsp lactis CIDCA 133 modulates response of human epithelial and dendritic cells infected with Bacillus cereus. Beneficial Microbes 7: 749-760.
'Lactobacillus delbrueckii subsp lactis CIDCA 133 modulates response of human epithelial and dendritic cells infected with Bacillus cereus ' () 7 Beneficial Microbes : 749 -760.
-
Sehgal, K., Dhodapkar, K.M. and Dhodapkar, M.V., 2014. Targeting human dendritic cells in situ to improve vaccines. Immunology Letters 162: 59-67.
'Targeting human dendritic cells in situ to improve vaccines ' () 162 Immunology Letters : 59 -67.
-
Shaw, D.M., Gaerthe, B., Leer, R.J., Van Der Stap, J.G., Smittenaar, C., Heijne Den Bak-Glashouwer, M., Thole, J.E., Tielen, F.J., Pouwels, P.H. and Havenith, C.E., 2000. Engineering the microflora to vaccinate the mucosa: serum immunoglobulin G responses and activated draining cervical lymph nodes following mucosal application of tetanus toxin fragment C-expressing lactobacilli. Immunology 100: 510-518.
'Engineering the microflora to vaccinate the mucosa: serum immunoglobulin G responses and activated draining cervical lymph nodes following mucosal application of tetanus toxin fragment C-expressing lactobacilli ' () 100 Immunology : 510 -518.
-
Sorvig, E., Mathiesen, G., Naterstad, K., Eijsink, V.G. and Axelsson, L., 2005. High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors. Microbiology 151: 2439-2449.
'High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors ' () 151 Microbiology : 2439 -2449.
-
Tauer, C., Heinl, S., Egger, E., Heiss, S. and Grabherr, R., 2014. Tuning constitutive recombinant gene expression in Lactobacillus plantarum. Microbial Cell Factories 13: 150.
'Tuning constitutive recombinant gene expression in Lactobacillus plantarum ' () 13 Microbial Cell Factories : 150.
-
Vlasova, A.N., Chattha, K.S., Kandasamy, S., Liu, Z., Esseili, M., Shao, L., Rajashekara, G. and Saif, L.J., 2013. Lactobacilli and bifidobacteria promote immune homeostasis by modulating innate immune responses to human rotavirus in neonatal gnotobiotic pigs. PLoS ONE 8: e76962.
'Lactobacilli and bifidobacteria promote immune homeostasis by modulating innate immune responses to human rotavirus in neonatal gnotobiotic pigs ' () 8 PLoS ONE : e76962.
-
Wang, L., Fouts, D.E., Starkel, P., Hartmann, P., Chen, P., Llorente, C., DePew, J., Moncera, K., Ho, S.B., Brenner, D.A., Hooper, L.V. and Schnabl, B., 2016a. Intestinal REG3 lectins protect against alcoholic steatohepatitis by reducing mucosa-associated microbiota and preventing bacterial translocation. Cell Host and Microbe 19: 227-239.
'Intestinal REG3 lectins protect against alcoholic steatohepatitis by reducing mucosa-associated microbiota and preventing bacterial translocation ' () 19 Cell Host and Microbe : 227 -239.
-
Wang, Z., Wang, L., Chen, Z., Ma, X., Yang, X., Zhang, J. and Jiang, Z., 2016b. In vitro evaluation of swine-derived Lactobacillus reuteri: probiotic properties and effects on intestinal porcine epithelial cells challenged with enterotoxigenic Escherichia coli K88. Journal of Microbiology and Biotechnology 26: 1018-1025.
'In vitro evaluation of swine-derived Lactobacillus reuteri: probiotic properties and effects on intestinal porcine epithelial cells challenged with enterotoxigenic Escherichia coli K88 ' () 26 Journal of Microbiology and Biotechnology : 1018 -1025.
-
Wen, K., Liu, F., Li, G., Bai, M., Kocher, J., Yang, X., Wang, H., Clark-Deener, S. and Yuan, L., 2015. Lactobacillus rhamnosus GG dosage affects the adjuvanticity and protection against rotavirus diarrhea in gnotobiotic pigs. Journal of Pediatric Gastroenterology and Nutrition 60: 834-843.
'Lactobacillus rhamnosus GG dosage affects the adjuvanticity and protection against rotavirus diarrhea in gnotobiotic pigs ' () 60 Journal of Pediatric Gastroenterology and Nutrition : 834 -843.
-
Wen, L.J., Hou, X.L., Wang, G.H., Yu, L.Y., Wei, X.M., Liu, J.K., Liu, Q. and Wei, C.H., 2012. Immunization with recombinant Lactobacillus casei strains producing K99, K88 fimbrial protein protects mice against enterotoxigenic Escherichia coli. Vaccine 30: 3339-3349.
'Immunization with recombinant Lactobacillus casei strains producing K99, K88 fimbrial protein protects mice against enterotoxigenic Escherichia coli ' () 30 Vaccine : 3339 -3349.
-
Wilson, R.A. and Francis, D.H., 1986. Fimbriae and enterotoxins associated with Escherichia coli serogroups isolated from pigs with colibacillosis. American Journal of Veterinary Research 47: 213-217.
'Fimbriae and enterotoxins associated with Escherichia coli serogroups isolated from pigs with colibacillosis ' () 47 American Journal of Veterinary Research : 213 -217.
-
Woting, A. and Blaut, M., 2016. The intestinal microbiota in metabolic disease. Nutrients 8(4): 202.
'The intestinal microbiota in metabolic disease ' () 8 Nutrients : 202.
-
Xia, P., Song, Y., Zou, Y., Yang, Y. and Zhu, G., 2015a. F4 enterotoxigenic Escherichia coli (ETEC) adhesion mediated by the major fimbrial subunit FaeG. Journal of Basic Microbiology.
'F4 enterotoxigenic Escherichia coli (ETEC) adhesion mediated by the major fimbrial subunit FaeG ', in Journal of Basic Microbiology , ().
-
Xia, P., Zou, Y., Wang, Y., Song, Y., Liu, W., Francis, D.H. and Zhu, G., 2015b. Receptor for the F4 fimbriae of enterotoxigenic Escherichia coli (ETEC). Applied Microbiology and Biotechnology 99: 4953-4959.
'Receptor for the F4 fimbriae of enterotoxigenic Escherichia coli (ETEC) ' () 99 Applied Microbiology and Biotechnology : 4953 -4959.
-
Yang, Y., Galle, S., Le, M.H., Zijlstra, R.T. and Ganzle, M.G., 2015. Feed fermentation with reuteran- and levan-producing Lactobacillus reuteri reduces colonization of weanling pigs by enterotoxigenic Escherichia coli. Applied Environmental Microbiology 81: 5743-5752.
'Feed fermentation with reuteran- and levan-producing Lactobacillus reuteri reduces colonization of weanling pigs by enterotoxigenic Escherichia coli ' () 81 Applied Environmental Microbiology : 5743 -5752.
-
Yu, Q., Yuan, L., Deng, J. and Yang, Q., 2015. Lactobacillus protects the integrity of intestinal epithelial barrier damaged by pathogenic bacteria. Frontiers in Cellular and Infection Microbiology 5: 26.
'Lactobacillus protects the integrity of intestinal epithelial barrier damaged by pathogenic bacteria ' () 5 Frontiers in Cellular and Infection Microbiology : 26.
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Alleviation of enterotoxigenic Escherichia coli challenge by recombinant Lactobacillus plantarum expressing a FaeG- and DC-targeting peptide fusion protein
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FaeG is the major subunit of K88 fimbriae. These cell surface attachments are considered to be the major virulence factor of enterotoxigenic Escherichia coli (ETEC), which causes diarrhoea in piglets. The use of dendritic cell-targeting peptide (DCpep) has been demonstrated to be an effective approach to enhance the immunity of vaccines. Lactobacillus plantarum is an attractive candidate for oral vaccination owing to its beneficial effects and safety. In this study, L. plantarum was employed to deliver a FaeG-DCpep fusion antigen, and the immune response in mice was evaluated. The synthesis of FaeG-DCpep dramatically increased the adhesion of recombinant L. plantarum (RLP) to IPEC-J2 cell surfaces, resulting in direct competition between L. plantarum and ETEC during adhesion assays. Significantly higher levels of body weight gain, sera immunoglobulin G and intestinal immunoglobulin A were observed in BALB/c mice immunised with RLP. In addition, the number of CD19+ B cells and CD11c+DC cells and the expression levels of several cytokines in the spleen and lymph nodes increased significantly compared to non-immunised mice. The oral administration of RLP also alleviated the symptoms of ETEC challenge, as shown by haematoxylin-eosin staining, indicating that RLP may be an efficient vaccine candidate.
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