Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Isolation of bacterial microbiota associated to honey bees and evaluation of potential biocontrol agents of Varroa destructor
in Beneficial MicrobesSearch for other papers by M.L. Saccà in
Current site
Google Scholar
Search for other papers by M. Lodesani in
Current site
Google Scholar
The honey bee parasitic mite Varroa destructor is one of the main causes of depopulation of bee colonies. Bacterial symbionts associated to honey bees are known to produce a variety of bioactive molecules that have been suggested to play a protective role against honey bee pathogens. We hypothesised that among these bacteria, those colonising the external body of honey bees, and therefore able to survive and reproduce in the hive environment outside the insect gut, may be good candidate biocontrol agents to be tested against V. destructor. The aim of this study was to isolate bacterial species from healthy honey bees and dead varroa mites and to evaluate the potential miticidal effect of their spent medium containing both bacterial metabolites and viable cells, with the final objective of finding a long-lasting solution for mite control. 61 bacterial strains belonging to the Firmicutes, Proteobacteria and Actinobacteria phyla were isolated from the surface of foragers, nurse bees and larvae collected in 10 different apiaries. The most common species was Lactobacillus kunkeei (62%). Growth capability of a selection of isolates was observed at 30 and 34 °C with 1% and 20% glucose and fructose. Laboratory bioassays were conducted by spraying mites with six-day-grown bacterial cultures containing 107 cfu/ml of four strains of L. kunkeei, Bacillus thuringiensis, Bifidobacterium asteroides and an Acetobacteraceae bacterium. The effect of each strain on varroa survival was tested independently. The first three caused 95-100% mortality of mites in 3 days, indicating a potential role as natural antagonists towards varroa. The mediation of pH of the bacterial cultures did not appear to be determinant in mite inhibition, suggesting the involvement of other modes of action against varroa. The exploitation of honey bee microbiota for controlling one of the major threats for honey bee health may be a promising approach deserving further investigation.
Kauf
Sofortzugang erwerben (PDF-Download und unbegrenzter Online-Zugang):
Institutszugang
Melden Sie sich mit Open Athens, Shibboleth oder Ihren institutionellen Anmeldedaten an.
Persönliche Anmeldung
Melden Sie sich mit Ihrem brill.com-Konto an
-
Aizenberg-Gershtein, Y., Izhaki, I. and Halpern, M., 2013. Do honeybees shape the bacterial community composition in floral nectar? PLoS ONE 8: e67556.
'Do honeybees shape the bacterial community composition in floral nectar? ' () 8 PLoS ONE : e67556.
-
Aksoy, H.M., Ozman-Sullivan, S.K., Ocal, H., Celik, N. and Sullivan, G.T., 2008. The effects of Pseudomonas putida biotype B on Tetranychus urticae (Acari: Tetranychidae). Experimental and Applied Acarology 46: 223-230. https://doi.org/10.1007/s10493-008-9155-9
-
Alberoni, D., Baffoni, L., Gaggìa, F., Ryan, P.M., Murphy, K., Ross, P.R., Stanton, C. and Di Gioia, D., 2018. Impact of beneficial bacteria supplementation on the gut microbiota, colony development and productivity of Apis mellifera L. Beneficial Microbes 9: 269-278. https://doi.org/10.3920/BM2017.0061
-
Alberoni, D., Gaggìa, F., Baffoni, L. and Di Gioia, D., 2016. Beneficial microorganisms for honey bees: problems and progresses. Applied Microbiology and Biotechnology 100: 9469-9482. https://doi.org/10.1007/s00253-016-7870-4
-
Alquisira-Ramírez, E.V., Paredes-Gonzalez, J.R., Hernández-Velázquez, V.M., Ramírez-Trujillo, J.A. and Peña-Chora, G., 2014. In vitro susceptibility of Varroa destructor and Apis mellifera to native strains of Bacillus thuringiensis. Apidologie 45: 707-718. https://doi.org/10.1007/s13592-014-0288-z
-
Alquisira-Ramírez, E.V., Peña-Chora, G., Hernández-Velázquez, V.M., Alvear-García, A., Arenas-Sosa, I. and Suarez-Rodríguez, R., 2017. Effects of Bacillus thuringiensis strains virulent to Varroa destructor on larvae and adults of Apis mellifera. Ecotoxicology and Environmental Safety 142: 69-78. https://doi.org/10.1016/j.ecoenv.2017.03.050
-
Anderson, K.E., Sheehan, T.H., Mott, B.M., Maes, P., Snyder, L., Schwan, M.R., Walton, A., Jones, B.M. and Corby-Harris, V., 2013. Microbial ecology of the hive and pollination landscape: bacterial associates from floral nectar, the alimentary tract and stored food of honey bees (Apis mellifera). PLoS ONE 8: e83125. https://doi.org/10.1371/journal.pone.0083125
-
Arredondo, D., Castelli, L., Porrini, M.P., Garrido, P.M., Eguaras, M.J., Zunino, P. and Antúnez, K., 2017. Lactobacillus kunkeei strains decreased the infection by honey bee pathogens Paenibacillus larvae and Nosema ceranae. Beneficial Microbes 9: 279-290. https://doi.org/10.3920/BM2017.0075
-
Asama, T., Arima, T.H., Gomi, T., Keishi, T., Tani, H., Kimura, Y., Tatefuji, T. and Hashimoto, K., 2015. Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults. Journal of Applied Microbiology 119: 818-826. https://doi.org/10.1111/jam.12889
-
Audisio, M.C., 2016. Gram-positive bacteria with probiotic potential for the Apis mellifera L. honey bee: the experience in the northwest of Argentina. Probiotics and Antimicrobial Proteins 9: 22-31. https://doi.org/10.1007/s12602-016-9231-0
-
Audisio, M.C., Sabaté, D.C. and Benítez-Ahrendts, M.R., 2015. Effect of Lactobacillus johnsonii CRL1647 on different parameters of honeybee colonies and bacterial populations of the bee gut. Beneficial Microbes 6: 687-695. https://doi.org/10.3920/BM2014.0155
-
Audisio, M.C., Torres, M.J., Sabaté, D.C., Ibarguren, C. and Apella, M.C., 2011. Properties of different lactic acid bacteria isolated from Apis mellifera L. bee-gut. Microbiological Research 166: 1-13. https://doi.org/10.1016/j.micres.2010.01.003
-
Baffoni, L., Gaggìa, F., Alberoni, D., Cabbri, R., Nanetti, A., Biavati, B. and Di Gioia, D., 2016. Effect of dietary supplementation of Bifidobacterium and Lactobacillus strains in Apis mellifera L. against Nosema ceranae. Beneficial Microbes 7: 45-51. https://doi.org/10.3920/BM2015.0085
-
Büchler, R. and Nanetti, A., 2016. Seasonal brood interruption study 2016/2017. Coloss Varroa control task force. Coloss, Institute of Bee Health, University of Bern, Bern, Switzerland. Available at: https://tinyurl.com/y3usl4py
-
Chandler, D., Sunderland, K.D., Ball, B.V. and Davidson, G., 2001. Prospective biological control agents of Varroa destructor n. sp., an important pest of the European honeybee, Apis mellifera, Biocontrol Science and Technology 11: 429-448. https://doi.org/10.1080/09583150120067472
-
Cordova-Kreylos, A.L., Fernandez, L.E., Koivunen, M., Yang, A., Flor-Weiler, L. and Marrone, P.G., 2013. Isolation and characterization of Burkholderia rinojensis sp. nov., a non-Burkholderia cepacia complex soil bacterium with insecticidal and miticidal activities. Applied and Environmental Microbiology 79: 7669-7678. https://doi.org/10.1128/AEM.02365-13
-
Crotti, E., Rizzi, A., Chouaia, B., Ricci, I., Favia, G., Alma, A., Sacchi, L., Bourtzis, K., Mandrioli, M., Cherif, A., Bandi, C. and Daffonchio, D., 2010. Acetic acid bacteria, newly emerging symbionts of insects. Applied and Environmental Microbiology 76: 6963-6970. https://doi.org/10.1128/AEM.01336-10
-
Crotti, E., Sansonno, L., Prosdocimi, E.M., Vacchini, V., Hamdi, C., Cherif, A., Gonella, E., Marzorati, M. and Balloi, A., 2013. Microbial symbionts of honeybees: a promising tool to improve honeybee health. New Biotechnology 30: 716-722. https://doi.org/10.1016/j.nbt.2013.05.004
-
De Miranda, J.R., Cordoni, G. and Budge, G., 2010. The acute bee paralysis virus-Kashmir bee virus-Israeli acute paralysis virus complex. Journal of Invertebrate Pathology 103: S30-47. https://doi.org/10.1016/j.jip.2009.06.014
-
Endo, A. and Salminen, S., 2013. Honeybees and beehives are rich sources for fructophilic lactic acid bacteria. Systematic and Applied Microbiology 36: 444-448. https://doi.org/10.1016/j.syapm.2013.06.002
-
Endo, A., Futagawa-Endo, Y. and Dicks, L.M.T., 2009. Isolation and characterization of fructophilic lactic acid bacteria from fructose-rich niches. Systematic and Applied Microbiology 32: 593-600. https://doi.org/10.1016/j.syapm.2009.08.002
-
Evans, J.D. and Armstrong, T.-N., 2006. Antagonistic interactions between honey bee bacterial symbionts and implications for disease. BMC Ecology 6: 4. https://doi.org/10.1186/1472-6785-6-4
-
Evans, J.D. and Lopez, D.L., 2004. Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae). Journal of Economic Entomology 97: 752-756.
'Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae) ' () 97 Journal of Economic Entomology : 752 -756.
-
Forsgren, E., Olofsson, T.C., Vásquez, A. and Fries, I., 2010. Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae. Apidologie 41: 99-108. https://doi.org/10.1051/apido/2009065
-
Giacobino, A., Pacini, A., Molineri, A., Rodriguez, G., Crisanti, P., Bulacio Cagnolo, N., Merke, J., Orellano, E., Bertozzi, E., Pietronave, H. and Signorini, M., 2018. Potential associations between the mite Varroa destructor and other stressors in honeybee colonies (Apis mellifera L.) in temperate and subtropical climate from Argentina. Preventive Veterinary Medicine 159: 143-152. https://doi.org/10.1016/j.prevetmed.2018.09.011
-
Gregorc, A., Alburaki, M., Werle, C., Knight, P.R. and Adamczyk, J., 2017. Brood removal or queen caging combined with oxalic acid treatment to control varroa mites (Varroa destructor) in honey bee colonies (Apis mellifera). Apidologie 48: 821-832. https://doi.org/10.1007/s13592-017-0526-2
-
Hammer, Ø., Harper, D.A.T. and Ryan, P.D., 2001. PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4(1): 1-9. https://doi.org/10.1016/j.bcp.2008.05.025
-
Heberle, H., Meirelles, G.V., Da Silva, F.R., Telles, G.P. and Minghim, R., 2015. InteractiVenn: a web-based tool for the analysis of sets through Venn diagrams. BMC Bioinformatics 16: 169. https://doi.org/10.1186/s12859-015-0611-3
-
Hrabak, J., 2004. The microorganisms isolated from the mites Varroa destructor and the verification of their pathogenity. Standing Commission of Bee Pathology. Available at: http://www.fiitea.org/foundation/files/090.pdf
-
Hubert, J., Bicianova, M., Ledvinka, O., Kamler, M., Lester, P.J., Nesvorna, M., Kopecky, J. and Erban, T., 2017. Changes in the bacteriome of honey bees associated with the parasite Varroa destructor, and pathogens Nosema and Lotmaria passim. Microbial Ecology 73: 685-698. https://doi.org/10.1007/s00248-016-0869-7
-
Hubert, J., Erban, T., Kamler, M., Kopecky, J., Nesvorna, M., Hejdankova, S., Titera, D., Tyl, J. and Zurek, L., 2015. Bacteria detected in the honeybee parasitic mite Varroa destructor collected from beehive winter debris. Journal of Applied Microbiology 119: 640-654. https://doi.org/10.1111/jam.12899
-
Janashia, I. and Alaux, C., 2016. Specific immune stimulation by endogenous bacteria in honey bees (Hymenoptera: Apidae). Journal of Economic Entomology 109: 1474-1477. https://doi.org/10.1093/jee/tow065
-
Janashia, I., Carminati, D., Rossetti, L., Zago, M., Fornasari, M.E., Haertlé, T., Chanishvili, N. and Giraffa, G., 2016. Characterization of fructophilic lactic microbiota of Apis mellifera from the Caucasus mountains. Annals of Microbiology 66: 1387-1395. https://doi.org/10.1007/s13213-016-1226-2
-
Killer, J., Dubná, S., Sedláček, I. and Švec, P., 2014. Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood. International Journal of Systematic and Evolutionary Microbiology 64: 152-157. https://doi.org/10.1099/ijs.0.053033-0
-
Kumar, S., Stecher, G. and Tamura, K., 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870-1874. https://doi.org/10.1093/molbev/msw054
-
Kwong, W.K. and Moran, N.A., 2016. Gut microbial communities of social bees. Nature Reviews Microbiology 14: 374.
'Gut microbial communities of social bees ' () 14 Nature Reviews Microbiology : 374.
-
Lamei, S., Stephan, J.G., Riesbeck, K., Vasquez, A., Olofsson, T., Nilson, B., De Miranda, J.R. and Forsgren, E., 2019. The secretome of honey bee-specific lactic acid bacteria inhibits Paenibacillus larvae growth. Journal of Apicultural Research 58: 405-412. https://doi.org/10.1080/00218839.2019.1572096
-
Lee, F.J., Miller, K.I., McKinlay, J.B. and Newton, I.L.G., 2018. Differential carbohydrate utilization and organic acid production by honey bee symbionts. FEMS Microbiology Ecology 94: fiy113. https://doi.org/10.1093/femsec/fiy113
-
Lee, K.V, Steinhauer, N., Rennich, K., Wilson, M.E., Tarpy, D.R., Caron, D.M., Rose, R., Delaplane, K.S., Baylis, K., Lengerich, E.J., Pettis, J., Skinner, J.A., Wilkes, J.T., Sagili, R. and Van Engelsdorp, D., 2015. A national survey of managed honey bee 2013-2014 annual colony losses in the USA. Apidologie 46: 292-305. https://doi.org/10.1007/s13592-015-0356-z
-
Lodesani, M. and Costa, C., 2005. Limits of chemotherapy in beekeeping: development of resistance and the problem of residues. Bee World 86: 102-109. https://doi.org/10.1080/0005772X.2005.11417324
-
Lodesani, M., Costa, C., Franceschetti, S., Bergomi, P., Galaverna, G. and Dall’Asta, C., 2017. Toxicity of destruxins against the parasitic mite Varroa destructor and its host Apis mellifera. Journal of Apicultural Research 56: 278-287. https://doi.org/10.1080/00218839.2017.1304611
-
Lodesani, M., Franceschetti, S. and Dall’Ollio, R., 2019. Evaluation of early spring bio-technical management techniques to control varroosis in Apis mellifera. Apidologie 50: 131-140. https://doi.org/10.1007/s13592-018-0621-z
-
Ludvigsen, J., Rangberg, A., Avershina, E., Sekelja, M., Kreibich, C., Amdam, G. and Rudi, K., 2015. Shifts in the midgut/pyloric microbiota composition within a honey bee apiary throughout a season. Microbes and Environments 30: 235-244. https://doi.org/10.1264/jsme2.ME15019
-
Maddaloni, M. and Pascual, D.W., 2015. Isolation of oxalotrophic bacteria associated with Varroa destructor mites. Letters in Applied Microbiology 61: 411-417. https://doi.org/10.1111/lam.12486
-
Maggi, M., Negri, P., Plischuk, S., Szawarski, N., De Piano, F., De Feudis, L., Eguaras, M. and Audisio, C., 2013. Effects of the organic acids produced by a lactic acid bacterium in Apis mellifera colony development, Nosema ceranae control and fumagillin efficiency. Veterinary Microbiology 167: 474-483. https://doi.org/10.1016/j.vetmic.2013.07.030
-
Marche, M.G., Satta, A., Floris, I., Pusceddu, M., Buffa, F. and Ruiu, L., 2019. Quantitative variation in the core bacterial community associated with honey bees from Varroa-infested colonies. Journal of Apicultural Research 58: 444-454. https://doi.org/10.1080/00218839.2019.1589669
-
Meikle, W.G., Sammataro, D., Neumann, P. and Pflugfelder, J., 2012. Challenges for developing pathogen-based biopesticides against Varroa destructor (Mesostigmata: Varroidae). Apidologie 43: 501-514. https://doi.org/10.1007/s13592-012-0118-0
-
Mutinelli, F., Costa, C., Lodesani, M., Baggio, A., Medrzycki, P., Formato, G. and Porrini, C., 2010. Honey bee colony losses in Italy. Journal of Apicultural Research 49: 119-120. https://doi.org/10.3896/IBRA.1.49.1.24
-
Nanetti, A., 1999. Oxalic acid for mite control – results and review. In: FAIR CT97-3686. Coordination in Europe of integrated control of Varroa mites in honey bee colonies. Appendix VI. Final Technical Report. European Commission, Brussels, Belgium, pp. 9-16.
'Oxalic acid for mite control – results and review ', () 9 -16.
-
Nanetti, A., Bartolomei, P., Bellato, S., De Salvio, M., Gattavecchia, E. and Ghini, R., 2003. Pharmacodynamics of oxalic acid in the honey bee colony. In: 38th Apimondia International Congress. Apimondia, Ljubljana, Slovenia, pp. 1-6. Available at: http://www.fiitea.org/foundation/files/164.pdf
-
Nanetti, A., Higes, M., Baracani, G. and Besana, A., 2012. Control de la varroa con ácido oxálico en combinación con la interrupción artificial de la puesta de cría. In: Proceedings of II Congreso Ibérico de Apicultura. October 18-20, 2012. Guadalajara, Spain.
Control de la varroa con ácido oxálico en combinación con la interrupción artificial de la puesta de cría
-
Nazzi, F., Brown, S.P., Annoscia, D., Del Piccolo, F., Di Prisco, G., Varricchio, P., Della Vedova, G., Cattonaro, F., Caprio, E. and Pennacchio, F., 2012. Synergistic parasite-pathogen interactions mediated by host immunity can drive the collapse of honeybee colonies. PLoS Pathogens 8: e1002735.
'Synergistic parasite-pathogen interactions mediated by host immunity can drive the collapse of honeybee colonies ' () 8 PLoS Pathogens : e1002735.
-
Neveling, D.P., Endo, A. and Dicks, L.M.T., 2012. Fructophilic Lactobacillus kunkeei and Lactobacillus brevis isolated from fresh flowers, bees and bee-hives. Current Microbiology 65: 507-515. https://doi.org/10.1007/s00284-012-0186-4
-
Olofsson, T.C. and Vásquez, A., 2008. Detection and identification of a novel lactic acid bacterial flora within the honey stomach of the honeybee Apis mellifera. Current Microbiology 57: 356-363. https://doi.org/10.1007/s00284-008-9202-0
-
Pakwan, C., Kaltenpoth, M., Weiss, B., Chantawannakul, P., Jun, G. and Disayathanoowat, T., 2018. Bacterial communities associated with the ectoparasitic mites Varroa destructor and Tropilaelaps mercedesae of the honey bee (Apis mellifera). FEMS Microbiology Ecology 94: fix160. https://doi.org/10.1093/femsec/fix160
-
Powell, J.E., Martinson, V.G., Urban-Mead, K. and Moran, N.A., 2014. Routes of acquisition of the gut microbiota of the honey bee Apis mellifera. Applied and Environmental Microbiology 80: 7378-7387. https://doi.org/10.1128/AEM.01861-14
-
Price-Whelan, A., Dietrich, L.E.P. and Newman, D.K., 2006. Rethinking ‘secondary’ metabolism: physiological roles for phenazine antibiotics. Nature Chemical Biology 2: 71-78. https://doi.org/10.1038/nchembio764
-
Qi, S., Zhu, L., Wang, D., Wang, C., Chen, X., Xue, X. and Wu, L., 2020. Flumethrin at honey-relevant levels induces physiological stresses to honey bee larvae (Apis mellifera L.) in vitro. Ecotoxicology and Environmental Safety 190: 110101. https://doi.org/10.1016/j.ecoenv.2019.110101
-
Raymann, K., Coon, K.L., Shaffer, Z., Salisbury, S. and Moran, N.A., 2018. Pathogenicity of Serratia marcescens strains in honey bees. mBio 9: e01649-18. https://doi.org/10.1128/mBio.01649-18
-
Ribière, C., Hegarty, C., Stephenson, H., Whelan, P. and O’Toole, P.W., 2019. Gut and whole-body microbiota of the honey bee separate thriving and non-thriving hives. Microbial Ecology 78: 195-205. https://doi.org/10.1007/s00248-018-1287-9
-
Rinkevich, F.D., 2020. Detection of amitraz resistance and reduced treatment efficacy in the varroa mite, Varroa destructor, within commercial beekeeping operations. PLoS ONE 15: e0227264. https://doi.org/10.1371/journal.pone.0227264
-
Rodríguez, M., Gerding, M. and France, A., 2009. Selection of entomopathogenic fungi to control Varroa destructor (Acari: Varroidae). Chilean Journal of Agricultural Research 69: 534-540. https://doi.org/10.4067/S0718-58392009000400008
-
Rokop, Z.P., Horton, M.A. and Newton, I.L.G., 2015. Interactions between cooccurring lactic acid bacteria in honey bee hives. Applied and Environmental Microbiology 81: 7261-7270. https://doi.org/10.1128/AEM.01259-15
-
Rosenkranz, P., Aumeier, P. and Ziegelmann, B., 2010. Biology and control of Varroa destructor. Journal of Invertebrate Pathology 103: S96-119. https://doi.org/10.1016/j.jip.2009.07.016
-
Sabate, D.C., Cruz, M.S., Benitez-Ahrendts, M.R. and Audisio, M.C., 2012. Beneficial effects of Bacillus subtilis subsp. subtilis Mori2, a honey-associated strain, on honeybee colony performance. Probiotics and Antimicrobial Proteins 4: 39-46. https://doi.org/10.1007/s12602-011-9089-0
-
Sandionigi, A., Vicario, S., Prosdocimi, E.M., Galimberti, A., Ferri, E., Bruno, A., Balech, B., Mezzasalma, V. and Casiraghi, M., 2015. Towards a better understanding of Apis mellifera and Varroa destructor microbiomes: introducing ‘phyloh’ as a novel phylogenetic diversity analysis tool. Molecular Ecology Resources 15: 697-710. https://doi.org/10.1111/1755-0998.12341
-
Schütte, C., Gols, R., Kleespies, R.G., Poitevin, O. and Dicke, M., 2008. Novel bacterial pathogen Acaricomes phytoseiuli causes severe disease symptoms and histopathological changes in the predatory mite Phytoseiulus persimilis (Acari, Phytoseiidae). Journal of Invertebrate Pathology 98: 127-135. https://doi.org/10.1016/j.jip.2008.03.006
-
Shaw, K.E., Davidson, G., Clark, S.J., Ball, B.V, Pell, J.K., Chandler, D. and Sunderland, K.D., 2002. Laboratory bioassays to assess the pathogenicity of mitosporic fungi to Varroa destructor (Acari: Mesostigmata), an ectoparasitic mite of the honeybee, Apis mellifera. Biological Control 24: 266-276. https://doi.org/10.1016/S1049-9644(02)00029-4
-
Shen, M., Cui, L., Ostiguy, N. and Cox-Foster, D., 2005. Intricate transmission routes and interactions between picorna-like viruses (Kashmir bee virus and sacbrood virus) with the honeybee host and the parasitic varroa mite. Journal of General Virology 86: 2281-2289. https://doi.org/10.1099/vir.0.80824-0
-
Torres, M.J., Rocha, V.F., and Audisio, M.C., 2016. Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica. Entomologia Experimentalis et Applicata 159: 347-353. https://doi.org/10.1111/eea.12445
-
Tsagou, V., Lianou, A., Lazarakis, D., Emmanouel, N. and Aggelis, G., 2004. Newly isolated bacterial strains belonging to Bacillaceae (Bacillus sp.) and Micrococcaceae accelerate death of the honey bee mite, Varroa destructor (V. jacobsoni), in laboratory assays. Biotechnology Letters 26: 529-532. https://doi.org/10.1023/B:BILE.0000019563.92959.0e
-
Tu, S., Qiu, X., Cao, L., Han, R., Zhang, Y. and Liu, X., 2010. Expression and characterization of the chitinases from Serratia marcescens GEI strain for the control of Varroa destructor, a honey bee parasite. Journal of Invertebrate Pathology 104: 75-82. https://doi.org/10.1016/j.jip.2010.02.002
-
Underwood, R. and Van Engelsdorp, D., 2007. Colony collapse disorder: have we seen this before? Bee Culture 35: 13-18.
'Colony collapse disorder: have we seen this before? ' () 35 Bee Culture : 13 -18.
-
Van der Geest, L.P.S., Elliot, S.L., Breeuwer, J.A.J. and Beerling, E.A.M., 2000. Diseases of mites. Experimental and Applied Acarology 24: 497-560. https://doi.org/10.1023/A:1026518418163
-
Van Engelsdorp, D., Evans, J.D., Saegerman, C., Mullin, C., Haubruge, E., Nguyen, B.K., Frazier, M., Frazier, J., Cox-Foster, D., Chen, Y., Underwood, R., Tarpy, D.R. and Pettis, J.S., 2009. Colony collapse disorder: a descriptive study. PLoS ONE 4: e6481.
'Colony collapse disorder: a descriptive study ' () 4 PLoS ONE : e6481.
-
Vanikova, S., Noskova, A., Pristas, P., Judova, J. and Javorsky, P., 2015. Heterotrophic bacteria associated with Varroa destructor mite. Apidologie 46: 369-379. https://doi.org/10.1007/s13592-014-0327-9
-
Vásquez, A., Forsgren, E., Fries, I., Paxton, R.J., Flaberg, E., Szekely, L. and Olofsson, T.C., 2012. Symbionts as major modulators of insect health: lactic acid bacteria and honeybees. PLoS ONE 7: e33188. https://doi.org/10.1371/journal.pone.0033188
-
Wright, E.S., Yilmaz, L.S. and Noguera, D.R., 2012. DECIPHER, a search-based approach to chimera identification for 16S rRNA sequences. Applied and Environmental Microbiology 78: 717-725. https://doi.org/10.1128/AEM.06516-11
-
Yoon, S.-H., Ha, S.-M., Kwon, S., Lim, J., Kim, Y., Seo, H. and Chun, J., 2017. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. International Journal of Systematic and Evolutionary Microbiology 67: 1613-1617. https://doi.org/10.1099/ijsem.0.001755
-
Yue, C. and Genersch, E., 2005. RT-PCR analysis of deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor). Journal of General Virology 86: 3419-3424. https://doi.org/10.1099/vir.0.81401-0
Supplementary Materials
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 892 | 330 | 22 |
| Gesamttextansichten | 47 | 19 | 0 |
| PDF-Downloads | 77 | 48 | 0 |
Isolation of bacterial microbiota associated to honey bees and evaluation of potential biocontrol agents of Varroa destructor
in Beneficial MicrobesSearch for other papers by M.L. Saccà in
Current site
Google Scholar
PubMed
Search for other papers by M. Lodesani in
Current site
Google Scholar
PubMed
The honey bee parasitic mite Varroa destructor is one of the main causes of depopulation of bee colonies. Bacterial symbionts associated to honey bees are known to produce a variety of bioactive molecules that have been suggested to play a protective role against honey bee pathogens. We hypothesised that among these bacteria, those colonising the external body of honey bees, and therefore able to survive and reproduce in the hive environment outside the insect gut, may be good candidate biocontrol agents to be tested against V. destructor. The aim of this study was to isolate bacterial species from healthy honey bees and dead varroa mites and to evaluate the potential miticidal effect of their spent medium containing both bacterial metabolites and viable cells, with the final objective of finding a long-lasting solution for mite control. 61 bacterial strains belonging to the Firmicutes, Proteobacteria and Actinobacteria phyla were isolated from the surface of foragers, nurse bees and larvae collected in 10 different apiaries. The most common species was Lactobacillus kunkeei (62%). Growth capability of a selection of isolates was observed at 30 and 34 °C with 1% and 20% glucose and fructose. Laboratory bioassays were conducted by spraying mites with six-day-grown bacterial cultures containing 107 cfu/ml of four strains of L. kunkeei, Bacillus thuringiensis, Bifidobacterium asteroides and an Acetobacteraceae bacterium. The effect of each strain on varroa survival was tested independently. The first three caused 95-100% mortality of mites in 3 days, indicating a potential role as natural antagonists towards varroa. The mediation of pH of the bacterial cultures did not appear to be determinant in mite inhibition, suggesting the involvement of other modes of action against varroa. The exploitation of honey bee microbiota for controlling one of the major threats for honey bee health may be a promising approach deserving further investigation.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 892 | 330 | 22 |
| Gesamttextansichten | 47 | 19 | 0 |
| PDF-Downloads | 77 | 48 | 0 |
