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Treatment with Bifidobacterium longum 51A attenuates intestinal damage and inflammatory response in experimental colitis

in Beneficial Microbes
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F.A. Abrantes Departamento de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais. Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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B.B. Nascimento Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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M.E.R. Andrade Departamento de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais. Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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P.A.V. de Barros Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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C.T. Cartelle Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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F.S. Martins Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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J.R. Nicoli Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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R.M.E. Arantes Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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S.V. Generoso Departamento de Nutrição, Escola de Enfermagem, Universidade Federal de Minas Gerais, Av Professor Alfredo Balena 190, Belo Horizonte, MG 30130-100, Brazil.

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S.O.A. Fernandes Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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V.N. Cardoso Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.

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This study evaluated the effects of Bifidobacterium longum 51A on the intestinal mucosa and inflammatory response in experimental colitis. Colitis was induced by administration of 3.5% dextran sodium sulphate (DSS) solution for 7 days. Two periods of administration were performed: treatment (T) group, mice received Bifidobacterium only during disease induction (7 days); total treatment (TT) group, mice received Bifidobacterium for 10 days before and during disease induction. The probiotic effects on intestinal permeability, inflammatory infiltrate, histological analysis, cytokines, chemokines and sIgA were evaluated. Bifidobacterium administration in the T group showed reduction in intestinal permeability and lower IL-1β, myeloperoxidase, and eosinophil peroxidase levels compared to those in the colitis group (P<0.05). Bifidobacterium administration in the TT group attenuated severe lesions in the colon and reduced eosinophil peroxidase level (P<0.05). B. longum 51A treatment modality was more effective than total treatment and reduced the inflammatory response and its consequences on intestinal epithelium.

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