Blueberries as an additive to increase the survival of Lactobacillus johnsonii N6.2 to lyophilisation
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Effective cultivation methods, total cost, and biomass preservation are key factors that have a significant impact on the commercialisation and effectiveness of probiotics, such as Lactobacillus. Sugar polymers, milk and whey proteins have been suggested as good additives for industrial preparations. Alternative compounds, such as phytophenols, are a more attractive option, given their potential benefits to human health. The overall goal of this study was to determine if the addition of blueberry phytophenols improves the survival of Lactobacillus johnsonii N6.2 during the freeze-drying process. The addition of blueberry aqueous extract (BAE) stimulated the growth of L. johnsonii under aerobic conditions and improved the stationary phase survival of the bacteria. Furthermore, the addition of BAE to the culture media improved the endurance of L. johnsonii N6.2 to freeze-drying stress, as well as to storage at 4 °C for up to 21 weeks. Moreover, blueberry extract performed more effectively as a lyophilising additive compared to skim milk and microencapsulation with whey protein/sodium alginate. In sum, this study demonstrates that BAE is an effective additive to increase the growth and survival of L. johnsonii N6.2 when added to the culture medium and/or used as a lyophilising preservative. Moreover, BAE or other polyphenols sources might likely enhance growth and increase survival of more probiotic lactic acid bacterial strains.
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Bhat, R., Suryanarayana, L.C., Chandrashekara, K.A., Krishnan, P., Kush, A. and Ravikumar, P., 2015. Lactobacillus plantarum mediated fermentation of Psidium guajava L. fruit extract. Journal of Bioscience and Bioengineering 119: 430-432.
'Lactobacillus plantarum mediated fermentation of Psidium guajava L. fruit extract ' () 119 Journal of Bioscience and Bioengineering : 430 -432.
-
Bravo-Ferrada, B.M., Brizuela, N., Gerbino, E., Gómez-Zavaglia, A., Semorile, L. and Tymczyszyn, E.E., 2015. Effect of protective agents and previous acclimation on ethanol resistance of frozen and freeze-dried Lactobacillus plantarum strains. Cryobiology 71: 522-528.
'Effect of protective agents and previous acclimation on ethanol resistance of frozen and freeze-dried Lactobacillus plantarum strains ' () 71 Cryobiology : 522 -528.
-
Bustamante, M., Oomah, B.D., Rubilar, M. and Shene, C., 2017. Effective Lactobacillus plantarum and Bifidobacterium infantis encapsulation with chia seed (Salvia hispanica L.) and flaxseed (Linum usitatissimum L.) mucilage and soluble protein by spray drying. Food Chemistry 216: 97-105.
'Effective Lactobacillus plantarum and Bifidobacterium infantis encapsulation with chia seed (Salvia hispanica L.) and flaxseed (Linum usitatissimum L.) mucilage and soluble protein by spray drying ' () 216 Food Chemistry : 97 -105.
-
Correa-Betanzo, J., Allen-Vercoe, E., McDonald, J., Schroeter, K., Corredig, M. and Paliyath, G., 2014. Stability and biological activity of wild blueberry (Vaccinium angustifolium) polyphenols during simulated in vitro gastrointestinal digestion. Food Chemistry 165: 522-531.
'Stability and biological activity of wild blueberry (Vaccinium angustifolium) polyphenols during simulated in vitro gastrointestinal digestion ' () 165 Food Chemistry : 522 -531.
-
Correa-Betanzo, J., Padmanabhan, P., Corredig, M., Subramanian, J. and Paliyath, G., 2015. Complex formation of blueberry (Vaccinium angustifolium) anthocyanins during freeze-drying and its influence on their biological activity. Journal of Agricultural and Food Chemistry 63: 2935-2946.
'Complex formation of blueberry (Vaccinium angustifolium) anthocyanins during freeze-drying and its influence on their biological activity ' () 63 Journal of Agricultural and Food Chemistry : 2935 -2946.
-
Costa, M.G.M., Ooki, G.N., Vieira, A.D.S., Bedani, R. and Saad, S.M.I., 2017. Synbiotic Amazonian palm berry (açai, Euterpe oleracea Mart.) ice cream improved Lactobacillus rhamnosus GG survival to simulated gastrointestinal stress. Food and Function 8: 731-740.
'Synbiotic Amazonian palm berry (açai, Euterpe oleracea Mart.) ice cream improved Lactobacillus rhamnosus GG survival to simulated gastrointestinal stress ' () 8 Food and Function : 731 -740.
-
Dong, L.-M., Jia, X.-C., Luo, Q.-W., Zhang, Q., Luo, B., Liu, W.-B., Zhang, X., Xu, Q.-L. and Tan, J.-W., 2017. Phenolics from Mikania micrantha and their antioxidant activity. Molecules 22: 1140. DOI: https://doi.org/10.3390/molecules22071140
-
Endo, A., Teräsjärvi, J. and Salminen, S., 2014. Food matrices and cell conditions influence survival of Lactobacillus rhamnosus GG under heat stresses and during storage. International Journal of Food Microbiology 174: 110-112.
'Food matrices and cell conditions influence survival of Lactobacillus rhamnosus GG under heat stresses and during storage ' () 174 International Journal of Food Microbiology : 110 -112.
-
Kechagia, M., Basoulis, D., Konstantopoulou, S., Dimitriadi, D., Gyftopoulou, K., Skarmoutsou, N. and Fakiri, E.M., 2013. Health benefits of probiotics: a review. ISRN Nutrition 2013: 481651.
'Health benefits of probiotics: a review ' () 2013 ISRN Nutrition : 481651.
-
Klaenhammer, T.R., Kleerebezem, M., Kopp, M.V. and Rescigno, M., 2012. The impact of probiotics and prebiotics on the immune system. Nature Reviews Immunology 12: 728-734.
'The impact of probiotics and prebiotics on the immune system ' () 12 Nature Reviews Immunology : 728 -734.
-
Lai, K.K., Lorca, G.L. and Gonzalez, C.F., 2009. Biochemical properties of two cinnamoyl esterases purified from a Lactobacillus johnsonii strain isolated from stool samples of diabetes-resistant rats. Applied and Environmental Microbiology 75: 5018-5024.
'Biochemical properties of two cinnamoyl esterases purified from a Lactobacillus johnsonii strain isolated from stool samples of diabetes-resistant rats ' () 75 Applied and Environmental Microbiology : 5018 -5024.
-
Lorca, G.L. and Font de Valdez, G., 2001. A low-pH-inducible, stationary-phase acid tolerance response in Lactobacillus acidophilus CRL 639. Current Microbiology 42: 21-5.
'A low-pH-inducible, stationary-phase acid tolerance response in Lactobacillus acidophilus CRL 639 ' () 42 Current Microbiology : 21 -5.
-
Marty-Teysset, C., De la Torre, F. and Garel, J., 2000. Increased production of hydrogen peroxide by Lactobacillus delbrueckii subsp. bulgaricus upon aeration: involvement of an NADH oxidase in oxidative stress. Applied and Environmental Microbiology 66: 262-267.
'Increased production of hydrogen peroxide by Lactobacillus delbrueckii subsp ' () 66 Applied and Environmental Microbiology : 262 -267.
-
Nishiyama, K., Nakazato, A., Ueno, S., Seto, Y., Kakuda, T., Takai, S., Yamamoto, Y. and Mukai, T., 2015. Cell surface-associated aggregation-promoting factor from Lactobacillus gasseri SBT2055 facilitates host colonization and competitive exclusion of Campylobacter jejuni. Molecular Microbiology 98: 712-726.
'Cell surface-associated aggregation-promoting factor from Lactobacillus gasseri SBT2055 facilitates host colonization and competitive exclusion of Campylobacter jejuni ' () 98 Molecular Microbiology : 712 -726.
-
Nya, E.J., 2015. Development of probiotics as biotechnology-driven product for reducing the incidence of gastrointestinal related disease. International Journal of Science and Research 4: 1748-1751.
'Development of probiotics as biotechnology-driven product for reducing the incidence of gastrointestinal related disease ' () 4 International Journal of Science and Research : 1748 -1751.
-
Rajam, R., Kumar, S.B., Prabhasankar, P. and Anandharamakrishnan, C., 2015. Microencapsulation of Lactobacillus plantarum MTCC 5422 in fructooligosaccharide and whey protein wall systems and its impact on noodle quality. Journal of Food Science and Technology 52: 4029-4041.
'Microencapsulation of Lactobacillus plantarum MTCC 5422 in fructooligosaccharide and whey protein wall systems and its impact on noodle quality ' () 52 Journal of Food Science and Technology : 4029 -4041.
-
Rodrigues, F.J., Omura, M.H., Cedran, M.F., Dekker, R.F.H., Barbosa-Dekker, A.M. and Garcia, S., 2017. Effect of natural polymers on the survival of Lactobacillus casei encapsulated in alginate microspheres. Journal of Microencapsulation 34: 431-439.
'Effect of natural polymers on the survival of Lactobacillus casei encapsulated in alginate microspheres ' () 34 Journal of Microencapsulation : 431 -439.
-
Saarela, M., Virkajärvi, I., Nohynek, L., Vaari, A. and Mättö, J., 2006. Fibres as carriers for Lactobacillus rhamnosus during freeze-drying and storage in apple juice and chocolate-coated breakfast cereals. International Journal of Food Microbiology 112: 171-178.
'Fibres as carriers for Lactobacillus rhamnosus during freeze-drying and storage in apple juice and chocolate-coated breakfast cereals ' () 112 International Journal of Food Microbiology : 171 -178.
-
Sánchez-Rangel, J.C., Benavides, J., Heredia, J.B., Cisneros-Zevallos, L. and Jacobo-Velázquez, D.A., 2013. The Folin-Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination. Analytical Methods 5: 5990.
'The Folin-Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination ' () 5 Analytical Methods : 5990.
-
Shiby, V.K. and Mishra, H.N., 2013. Fermented milks and milk products as functional foods â a review. Critical Reviews in Food Science and Nutrition 53: 482-496.
'Fermented milks and milk products as functional foods â a review ' () 53 Critical Reviews in Food Science and Nutrition : 482 -496.
-
Valladares, R., Bojilova, L., Potts, A.H., Cameron, E., Gardner, C., Lorca, G. and Gonzalez, C.F., 2013. Lactobacillus johnsonii inhibits indoleamine 2,3-dioxygenase and alters tryptophan metabolite levels in BioBreeding rats. FASEB Journal 27: 1711-1720.
'Lactobacillus johnsonii inhibits indoleamine 2,3-dioxygenase and alters tryptophan metabolite levels in BioBreeding rats ' () 27 FASEB Journal : 1711 -1720.
-
Valladares, R., Sankar, D., Li, N., Williams, E., Lai, K.-K., Abdelgeliel, A.S., Gonzalez, C.F., Wasserfall, C.H., Larkin, J., Schatz, D., Atkinson, M.A., Triplett, E.W., Neu, J. and Lorca, G.L., 2010. Lactobacillus johnsonii N6.2 mitigates the development of type 1 diabetes in BB-DP rats. PLoS ONE 5: e10507.
'Lactobacillus johnsonii N6.2 mitigates the development of type 1 diabetes in BB-DP rats ' () 5 PLoS ONE : e10507.
-
Valladares, R.B., Graves, C., Wright, K., Gardner, C.L., Lorca, G.L. and Gonzalez, C.F., 2015. H2O2 production rate in Lactobacillus johnsonii is modulated via the interplay of a heterodimeric flavin oxidoreductase with a soluble 28 Kd PAS domain containing protein. Frontiers in Microbiology 6: 716.
'H2O2 production rate in Lactobacillus johnsonii is modulated via the interplay of a heterodimeric flavin oxidoreductase with a soluble 28 Kd PAS domain containing protein ' () 6 Frontiers in Microbiology : 716.
-
Van de Guchte, M., Serror, P., Chervaux, C., Smokvina, T., Ehrlich, S.D. and Maguin, E., 2002. Stress responses in lactic acid bacteria. Antonie van Leeuwenhoek 82: 187-216.
'Stress responses in lactic acid bacteria ' () 82 Antonie van Leeuwenhoek : 187 -216.
-
Zheng, X., Fu, N., Duan, M., Woo, M.W., Selomulya, C. and Chen, X.D., 2015. The mechanisms of the protective effects of reconstituted skim milk during convective droplet drying of lactic acid bacteria. Food Research International 76: 478-488.
'The mechanisms of the protective effects of reconstituted skim milk during convective droplet drying of lactic acid bacteria ' () 76 Food Research International : 478 -488.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 419 | 170 | 15 |
| å ¨ææµè§æ¬¡æ° | 10 | 3 | 0 |
| PDFä¸è½½æ¬¡æ° | 11 | 0 | 0 |
Blueberries as an additive to increase the survival of Lactobacillus johnsonii N6.2 to lyophilisation
äºBeneficial MicrobesSearch for other papers by D. Cai in
Current site
Google Scholar
PubMed
Search for other papers by N.A. Harrison in
Current site
Google Scholar
PubMed
Search for other papers by D.N. Kling in
Current site
Google Scholar
PubMed
Search for other papers by C.F. Gonzalez in
Current site
Google Scholar
PubMed
Search for other papers by G.L. Lorca in
Current site
Google Scholar
PubMed
Effective cultivation methods, total cost, and biomass preservation are key factors that have a significant impact on the commercialisation and effectiveness of probiotics, such as Lactobacillus. Sugar polymers, milk and whey proteins have been suggested as good additives for industrial preparations. Alternative compounds, such as phytophenols, are a more attractive option, given their potential benefits to human health. The overall goal of this study was to determine if the addition of blueberry phytophenols improves the survival of Lactobacillus johnsonii N6.2 during the freeze-drying process. The addition of blueberry aqueous extract (BAE) stimulated the growth of L. johnsonii under aerobic conditions and improved the stationary phase survival of the bacteria. Furthermore, the addition of BAE to the culture media improved the endurance of L. johnsonii N6.2 to freeze-drying stress, as well as to storage at 4 °C for up to 21 weeks. Moreover, blueberry extract performed more effectively as a lyophilising additive compared to skim milk and microencapsulation with whey protein/sodium alginate. In sum, this study demonstrates that BAE is an effective additive to increase the growth and survival of L. johnsonii N6.2 when added to the culture medium and/or used as a lyophilising preservative. Moreover, BAE or other polyphenols sources might likely enhance growth and increase survival of more probiotic lactic acid bacterial strains.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 419 | 170 | 15 |
| å ¨ææµè§æ¬¡æ° | 10 | 3 | 0 |
| PDFä¸è½½æ¬¡æ° | 11 | 0 | 0 |
