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Characterisation of the heat-stable bacteriocin-producing and vancomycin-sensitive Pediococcus pentosaceus CFR B19 isolated from beans

in Beneficial Microbes
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S. Venkateshwari Food Microbiology Department, Central Food Technological Research Institute, KRS Road, Mysore 570020, India; svnvijayendra@yahoo.com

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P.M. Halami Food Microbiology Department, Central Food Technological Research Institute, KRS Road, Mysore 570020, India; svnvijayendra@yahoo.com

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S.V.N. Vijayendra Food Microbiology Department, Central Food Technological Research Institute, KRS Road, Mysore 570020, India; svnvijayendra@yahoo.com

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Abstract

A bacteriocin-producing lactic culture with antilisterial activity was isolated from beans and identified as Pediococcus pentosaceus CFR B19. It was able to grow and produce bacteriocin at 41 °C but not at 50 °C. This isolate was found to be sensitive to vancomycin and produced heat-stable (at 121 °C for 15 min) bacteriocin. Molecular weight of the purified bacteriocin was found to be ~4.8 kDa. This isolate can be used as a starter culture or co-culture in fermented milk products and the bacteriocin can be used as a natural preservative in various food products.

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