Dissociation of Actomyosin into Actin and Myosin and Liberation of Actomyosin from Myofibrils Induced by Various Phosphates

M. Matsuishi; K. Kanno; M. Sawada; I. Suzuki; A. Okitani

Dissociation of Actomyosin into Actin and Myosin and Liberation of Actomyosin from Myofibrils Induced by Various Phosphates

In: 63rd International Congress of Meat Science and Technology

Authors:

M. Matsuishi

M. Matsuishi School of Food Science and Technology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan; matmas@nvlu.ac.jp

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K. Kanno

K. Kanno School of Food Science and Technology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan; matmas@nvlu.ac.jp

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M. Sawada

M. Sawada School of Food Science and Technology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan; matmas@nvlu.ac.jp

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I. Suzuki

I. Suzuki School of Food Science and Technology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan; matmas@nvlu.ac.jp

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A. Okitani

A. Okitani School of Food Science and Technology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan; matmas@nvlu.ac.jp

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Type:: Chapter

Pages:: 844–845

DOI:: https://doi.org/10.3920/9789086868605_377

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Aim of this study is to examine whether various phosphates dissociate free actomyosin or actomyosin restrained in myofibrils by gel permeation chromatography and SDS-PAGE. Eight millimolar pyrophosphate (PYP), tripolyphosphate (TRP) or inosine-5’-monophophate (IMP) dissociated actomyosin in 0.6 M KCl into myosin and actin. PYP or IMP dissociated actomyosin in 0.2 M KCl into myosin and actin, while TRP solubilized whole actomyosin without dissociation. PYP or TRP liberated whole actomyosin without dissociation from myofibrils, although IMP did not. These results suggested that PYP and TRP release actomyosin from particular restraints in myofibrils, however released actomyosin might not be dissociated into actin and myosin because of another binding between both proteins which cannot be cleaved by PYP and TRP.

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

E-Book ISBN:: 9789086868605

Publisher:: Wageningen Academic

Print Publication Date:: 15 Nov 2017

Subjects
- Life Sciences

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Dissociation of Actomyosin into Actin and Myosin and Liberation of Actomyosin from Myofibrils Induced by Various Phosphates

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

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