Use of Liquid Isoelectric Focusing (Offgel) to Study Changes in Muscle Proteome During Bovine Meat Ageing

L.R. Beldarrain; N. Aldai; E. Sentandreu; M.A. Sentandreu

Use of Liquid Isoelectric Focusing (Offgel) to Study Changes in Muscle Proteome During Bovine Meat Ageing

In: 63rd International Congress of Meat Science and Technology

Authors:

L.R. Beldarrain

L.R. Beldarrain ¹Instituto de Agroquímica y Tecnología de Alimentos (CSIC). 46980 Paterna (Valencia), Spain
²Lascaray Research Centre (UPV-EHU). Avda. Miguel de Unamuno 3.01006 Vitoria-Gasteiz, Spain

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N. Aldai

N. Aldai ²Lascaray Research Centre (UPV-EHU). Avda. Miguel de Unamuno 3.01006 Vitoria-Gasteiz, Spain

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E. Sentandreu

E. Sentandreu ³Instituto de Investigación Sanitaria La Fe. Avda. Fernando Abril Martorell 106 Torre A. 46026 Valencia, Spain; ciesen@iata.csic.es

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, and

M.A. Sentandreu

M.A. Sentandreu ¹Instituto de Agroquímica y Tecnología de Alimentos (CSIC). 46980 Paterna (Valencia), Spain

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Type:: Chapter

Pages:: 840–841

DOI:: https://doi.org/10.3920/9789086868605_375

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In this work, post mortem evolution of the myofibrillar protein fraction from bovine Longissimus thoracis muscle was studied. Myofibrillar fraction from samples obtained at different post mortem times was fractionated by liquid isoelectric focusing in the pH range 4 to 7, and further analyzed by SDS-PAGE. Spots of interest were identified by liquid chromatography coupled to tandem mass spectrometry. Profiles were highly reproducible, so variations over the different ageing times were easily identified. The fraction showing the most significant changes along the process (P<0.01) mainly comprised metabolic proteins, which may appear in the myofibrillar fraction because of changes in solubility. This promising technique is a step forward in the design of more efficient and easily automated methodologies in proteomics.

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

E-Book ISBN:: 9789086868605

Publisher:: Wageningen Academic

Print Publication Date:: 15 Nov 2017

Subjects
- Life Sciences

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Use of Liquid Isoelectric Focusing (Offgel) to Study Changes in Muscle Proteome During Bovine Meat Ageing

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

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