Investigation of Proteomic Changes and Quality of Sheep (Ovis Aries) Meat Due to Electrical Stunning

M. Kiran; B.M. Naveena; R. Srikanth; C. Venkatesh; Y. Praveen Kumar; V.V. Kulkarni

Investigation of Proteomic Changes and Quality of Sheep (Ovis Aries) Meat Due to Electrical Stunning

In: 63rd International Congress of Meat Science and Technology

Authors:

M. Kiran

M. Kiran ¹Department of Livestock Products Technology, Veterinary College Bidar, KVAFSU, India

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B.M. Naveena

B.M. Naveena ²National Research Centre on Meat, Chengicherla, Hyderabad, India

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R. Srikanth

R. Srikanth ³Proteomics Lab, National Centre for Cell Sciences, Pune, India; kiranm.321@rediffmail.com

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C. Venkatesh

C. Venkatesh ³Proteomics Lab, National Centre for Cell Sciences, Pune, India; kiranm.321@rediffmail.com

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Y. Praveen Kumar

Y. Praveen Kumar ²National Research Centre on Meat, Chengicherla, Hyderabad, India

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V.V. Kulkarni

V.V. Kulkarni ²National Research Centre on Meat, Chengicherla, Hyderabad, India

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Type:: Chapter

Pages:: 807–810

DOI:: https://doi.org/10.3920/9789086868605_361

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The present study investigates changes in bleeding efficiency, physicochemical characteristics, and muscle proteome of sheep subjected to either electrical stunning and slaughtering or slaughter without any stunning (halal). Higher (P<0.05) pH, water holding capacity, cooking loss and Warner-Bratzler shear force was observed in stunned samples, whereas higher (P<0.05) blood loss and Hunter lab a* values were observed in non-stunned meat. The 2DE gel analysis detected approximately 377 protein spots in which 243 (119 up regulated and 124 down regulated) protein spots were significantly (P≤0.05) altered with a fold change ratio ≥1.5/≤1.5. The in-gel digestion and MALDI-TOF/TOF MS analysis of statistically significant protein spots revealed 35 non-redundant proteins out of which 26 were up-regulated and 9 were down-regulated. Biological information such as pathways, protein classes, cellular components, biological processes, and molecular functions was obtained by PANTHER analysis. Our findings demonstrated that slaughtering of sheep without stunning resulted in changes of the abundance of proteins involved in catalytic, structural, and stress related processes. This understanding of protein alterations in sheep slaughtered with and without stunning have the potential to act as possible signature for animal welfare index.

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

E-Book ISBN:: 9789086868605

Publisher:: Wageningen Academic

Print Publication Date:: 15 Nov 2017

Subjects
- Life Sciences

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Investigation of Proteomic Changes and Quality of Sheep (Ovis Aries) Meat Due to Electrical Stunning

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

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