Improved Plasma Recovery by Means of Red Cell Crenation Assisted by Rsm Approach

C. Álvarez; A.M. Mullen

Improved Plasma Recovery by Means of Red Cell Crenation Assisted by Rsm Approach

In: 63rd International Congress of Meat Science and Technology

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C. Álvarez

C. Álvarez Department of Food Quality and Sensory Science, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland; carlos.alvarez@teagasc.ie

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A.M. Mullen

A.M. Mullen Department of Food Quality and Sensory Science, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland; carlos.alvarez@teagasc.ie

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Type:: Chapter

Pages:: 616–617

DOI:: https://doi.org/10.3920/9789086868605_274

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Blood plasma is a rich-protein liquid which has many applications in food, research, pet food and animal feed industries. However, for some applications, the haemoglobin level in plasma needs to be very low to prevent, for example, interference with other proteins, color changes or bitter/metallic taste. Plasma is obtained mainly by centrifugation, but as the processing volume increases, the haemoglobin content increases as well, and the plasma quality is considered poor. In this research a novel process, based on red cells crenation, is presented as a method to obtain low-haemoglobin content plasma, even when large volumes are centrifuged. Four factors were considered to affect the plasma quality: processing time and volume, centrifugation force, and concentration of phosphate saline buffer in plasma. Results showed that 10% v/v 10X-PBS treated-blood samples were able to generate better quality plasma than untreated samples regardless the speed, volume or time employed. Plasma obtained displayed similar functional properties as controls; even more ethyl fractionation following Cohn’s method generated rich protein fraction with identical SDS-PAGE profile than controls.

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

E-Book ISBN:: 9789086868605

Publisher:: Wageningen Academic

Print Publication Date:: 15 Nov 2017

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- Life Sciences

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Improved Plasma Recovery by Means of Red Cell Crenation Assisted by Rsm Approach

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

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