An Effect of Gas Stunning Regimes on Changes in Post Mortem Characteristics in the Conditions of a Model Experiment

A.I. Sinichkina; T.M. Mittelstein; I.V. Kozyrev; A.B. Lisitsyn; A.A. Semenova

An Effect of Gas Stunning Regimes on Changes in Post Mortem Characteristics in the Conditions of a Model Experiment

In: 63rd International Congress of Meat Science and Technology

Authors:

A.I. Sinichkina

A.I. Sinichkina V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia; pervichka@vniimp.ru

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T.M. Mittelstein

T.M. Mittelstein V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia; pervichka@vniimp.ru

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I.V. Kozyrev

I.V. Kozyrev V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia; pervichka@vniimp.ru

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A.B. Lisitsyn

A.B. Lisitsyn V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia; pervichka@vniimp.ru

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A.A. Semenova

A.A. Semenova V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia; pervichka@vniimp.ru

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Type:: Chapter

Pages:: 441–443

DOI:: https://doi.org/10.3920/9789086868605_195

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A model experiment on the laboratory rats was carried out using a CO₂ chamber for euthanasia and varying the flow rate of carbon dioxide (0.3 l/min. and 1.5 l/ min) within the framework of the study on animal stress under exposure to gas mixtures. After stunning, the animals were weighed and decapitated; the bleeding time was measured and serum biochemical analysis was carried out. The comparative weight of internal organs (heart, lung, liver, kidney, adrenal gland, spleen, brain) was measured. The blood levels of triglycerides, glucose and creatinine, and the activity of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) were determined. It was established that accelerated supply of carbon dioxide (1.5 l/min) allowed reducing exposure time until loss of sensitivity in animals on average by 378 s, ensured more complete bleeding and less damage of the internal organs as well as less stress level, which was evident from the activity of lactate dehydrogenase and creatine phosphokinase, as well as the blood level of creatinine.

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

E-Book ISBN:: 9789086868605

Publisher:: Wageningen Academic

Print Publication Date:: 15 Nov 2017

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- Life Sciences

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An Effect of Gas Stunning Regimes on Changes in Post Mortem Characteristics in the Conditions of a Model Experiment

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63rd International Congress of Meat Science and Technology

Nurturing locally, growing globally

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