Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Bovine hepatic differential gene expression in response to perennial ryegrass staggers
in World Mycotoxin JournalSearch for other papers by L. Li in
Current site
Google Scholar
Search for other papers by D.D. Tanaree in
Current site
Google Scholar
Search for other papers by Y. Di in
Current site
Google Scholar
Search for other papers by C.T. Estill in
Current site
Google Scholar
Search for other papers by J.M. Duringer in
Current site
Google Scholar
Search for other papers by L.L. Blythe in
Current site
Google Scholar
Search for other papers by A. Galen in
Current site
Google Scholar
Search for other papers by S. Livesay in
Current site
Google Scholar
Search for other papers by A.M. Craig in
Current site
Google Scholar
>‘Perennial ryegrass staggers’ is a neurological condition characterised by muscular tremors and ataxia in livestock that ingest the indole diterpene lolitrem B from endophyte-infected perennial ryegrass. While the neurotoxic mechanism of action of ryegrass staggers has been defined, little is known about metabolic and other molecular processes that lolitrem B may affect in livestock. The objective of this study was to characterise differential gene expression in the liver of animals fed lolitrem B-containing feed over an extended period of time. Eighteen steers were fed one of three rations (n=6/group) containing lolitrem B (247, 1,554 or 2,256 μg/kg (dry matter basis)) over 64 days in a double-blind study. Microarray and confirmatory qPCR were performed to evaluate the hepatic gene expression profile from biopsies taken on days 0 and 64. Widespread perturbation of gene expression was observed in all groups receiving lolitrem B, with a total of 152 differential genes identified (false discovery rate ≤0.05). This suggests that chronic exposure to lolitrem B, even at levels below the current threshold of toxicity (2,000 μg/kg lolitrem B), can perturb many genes, biological processes and pathways. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that many of these genes were categorised under lipid/steroid biosynthesis/metabolism and oxidation-reduction. Specifically, genes involved in the biosynthesis pathway of ceramide, a sphingolipid molecule (ACSS2,LASS6 andSCD) and changes in neurosignaling through alteration of nitric oxide synthase activity (ARG1 andGPX4) were up-regulated. Future work should focus on the overall balance between ceramide and its metabolites and antioxidants/oxidants in a variety of body matrices in animals with perennial ryegrass staggers, to determine how these compounds contribute to the overall etiology of this disease.
Kauf
Sofortzugang erwerben (PDF-Download und unbegrenzter Online-Zugang):
Institutszugang
Melden Sie sich mit Open Athens, Shibboleth oder Ihren institutionellen Anmeldedaten an.
Persönliche Anmeldung
Melden Sie sich mit Ihrem brill.com-Konto an
-
Alabdouli, K., Blythe, L.L., Duringer, J.M., Elkhouly, A., Kassab, A., Askar, M., Mohammed, E.E., Al-Juboori, A. and Craig, A.M., 2014. Physiological effects of endophyte-infected perennial ryegrass straw on female camels in the Middle East. Emirates Journal of Food and Agriculture 26: 82-92.
'Physiological effects of endophyte-infected perennial ryegrass straw on female camels in the Middle East ' () 26 Emirates Journal of Food and Agriculture : 82 -92.
-
Alexa, A., Rahnenführer, J. and Lengauer, T., 2006. Improved scoring of functional groups from gene expression data by decorrelating GO graph structure. Bioinformatics 22: 1600-1607.
'Improved scoring of functional groups from gene expression data by decorrelating GO graph structure ' () 22 Bioinformatics : 1600 -1607.
-
Bartók, T., Szécsi, Á., Juhász, K., Bartók, M. and Mesterházy, Á., 2013. ESI-MS and MS/MS identification of the first ceramide analogues of fumonisin B1 mycotoxin from aFusarium verticillioides culture following RP-HPLC separation. Food Additives and Contaminants: Part A 30: 1651-1659.
'ESI-MS and MS/MS identification of the first ceramide analogues of fumonisin B1 mycotoxin from a Fusarium verticillioides culture following RP-HPLC separation ' () 30 Food Additives and Contaminants: Part A : 1651 -1659.
-
Behrouzian, B. and Buist, P.H., 2002. Fatty acid desaturation: variations on an oxidative theme. Current Opinion in Chemical Biology 6: 577-582.
'Fatty acid desaturation: variations on an oxidative theme ' () 6 Current Opinion in Chemical Biology : 577 -582.
-
Benjamini, Y. and Hochberg, Y., 1995. Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society Series B 57: 289-300.
'Controlling the false discovery rate: a practical and powerful approach to multiple testing ' () 57 Journal of the Royal Statistical Society Series B : 289 -300.
-
Blythe, L.L., Estill, C., Males, J. and Craig, A.M., 2007. Determination of the toxic threshold of lolitrem B in cattle eating endophyte-infected perennial ryegrass. In: Popay, A.J. and Thom, E. (eds.) New Zealand Grassland Association: Endophyte Symposium. New Zealand Grassland Association, Dunedin, New Zealand, pp. 399-402.
New Zealand Grassland Association: Endophyte Symposium , () 399 -402.
-
Bucci, T.J., Howard, P.C., Tolleson, W.H., Laborde, J.B. and Hansen, D.K., 1998. Renal effects of fumonisin mycotoxins in animals. Toxicologic Pathology 26: 160-164.
'Renal effects of fumonisin mycotoxins in animals ' () 26 Toxicologic Pathology : 160 -164.
-
Cheeke, P.R., 1995. Endogenous toxins and mycotoxins in forage grasses and their effects on livestock. Journal of Animal Science 73: 909-918.
'Endogenous toxins and mycotoxins in forage grasses and their effects on livestock ' () 73 Journal of Animal Science : 909 -918.
-
Cutler, R.G., Kelly, J., Storie, K., Pedersen, W.A., Tammara, A., Hatanpaa, K., Troncoso, J.C. and Mattson, M.P., 2004. Involvement of oxidative stress-induced abnormalities in ceramide and cholesterol metabolism in brain aging and Alzheimer's disease. Proceedings of the National Academy of Sciences of the USA 101: 2070-2075.
'Involvement of oxidative stress-induced abnormalities in ceramide and cholesterol metabolism in brain aging and Alzheimer's disease ' () 101 Proceedings of the National Academy of Sciences of the USA : 2070 -2075.
-
Dalziel, J.E., Finch, S.C. and Dunlop, J., 2005. The fungal neurotoxin lolitrem B inhibits the function of human large conductance calcium-activated potassium channels. Toxicology Letters 155: 421-426.
'The fungal neurotoxin lolitrem B inhibits the function of human large conductance calcium-activated potassium channels ' () 155 Toxicology Letters : 421 -426.
-
Delker, S.L., Xue, F., Li, H., Jamal, J., Silverman, R.B. and Poulos, T.L., 2010. Role of zinc in isoform-selective inhibitor binding to neuronal nitric oxide synthase. Biochemistry 49: 10803-10810.
'Role of zinc in isoform-selective inhibitor binding to neuronal nitric oxide synthase ' () 49 Biochemistry : 10803 -10810.
-
Devlin, T.M., 2010. Textbook of biochemistry with clinical correlations. John Wiley & Sons, New York, NY, USA.
Textbook of biochemistry with clinical correlations , ().
-
DiMenna, M.E., Finch, S.C., Popay, A.J. and Smith, B.L., 2012. A review of theNeotyphodium lolii/Lolium perenne symbiosis and its associated effects on animal and plant health, with particular emphasis on ryegrass staggers. New Zealand Veterinary Journal 60: 315-328.
'A review of the Neotyphodium lolii/Lolium perenne symbiosis and its associated effects on animal and plant health, with particular emphasis on ryegrass staggers ' () 60 New Zealand Veterinary Journal : 315 -328.
-
Dobashi, K., Asayama, K., Nakane, T., Kodera, K., Hayashibe, H. and Nakazawa, S., 2001. Induction of glutathione peroxidase in response to inactivation by nitric oxide. Free Radical Research 35: 319-327.
'Induction of glutathione peroxidase in response to inactivation by nitric oxide ' () 35 Free Radical Research : 319 -327.
-
Dobrzyn, A., Dobrzyn, P., Lee, S.-H., Miyazaki, M., Cohen, P., Asilmaz, E., Hardie, D.G., Friedman, J.M. and Ntambi, J.M., 2005. Stearoyl-CoA desaturase-1 deficiency reduces ceramide synthesis by downregulating serine palmitoyltransferase and increasing β-oxidation in skeletal muscle. American Journal of Physiology – Endocrinology and Metabolism 288: E599-E607.
'Stearoyl-CoA desaturase-1 deficiency reduces ceramide synthesis by downregulating serine palmitoyltransferase and increasing β-oxidation in skeletal muscle ' () 288 American Journal of Physiology – Endocrinology and Metabolism : E599 -E607.
-
Doi, K. and Uetsuka, K., 2011. Mechanisms of mycotoxin-induced neurotoxicity through oxidative stress-associated pathways. International Journal of Molecular Sciences 12: 5213-5237.
'Mechanisms of mycotoxin-induced neurotoxicity through oxidative stress-associated pathways ' () 12 International Journal of Molecular Sciences : 5213 -5237.
-
Enna, S.J., 2001. GABAB Receptor Signaling Pathways. In: Möhler, P.D.H. (ed.) Pharmacology of GABA and glycine neurotransmission. Springer, Berlin, Germany, pp. 329-342.
Pharmacology of GABA and glycine neurotransmission , () 329 -342.
-
Fisher, M.J., Bohnert, D.W., Ackerman, C.J., Schauer, C.S., DelCurto, T., Craig, A.M., Vanzant, E., Harmon, D.L. and Schrick, F.N., 2004. Evaluation of perennial ryegrass straw as a forage source for ruminants. Journal of Animal Science 82: 2175-2184.
'Evaluation of perennial ryegrass straw as a forage source for ruminants ' () 82 Journal of Animal Science : 2175 -2184.
-
Galey, F.D., Tracy, M.L., Craigmill, A.L., Barr, B.C., Markegard, G., Peterson, R. and O'Connor, M., 1991. Staggers induced by consumption of perennial ryegrass in cattle and sheep from northern California. Journal of the American Veterinary Medical Association 199: 466-470.
'Staggers induced by consumption of perennial ryegrass in cattle and sheep from northern California ' () 199 Journal of the American Veterinary Medical Association : 466 -470.
-
Gallagher, R.T. and Hawkes, A.D., 1986. The potent tremorgenic neurotoxins lolitrem-B and aflatrem – a comparison of the tremor response in mice. Experientia 42: 823-825.
'The potent tremorgenic neurotoxins lolitrem-B and aflatrem – a comparison of the tremor response in mice ' () 42 Experientia : 823 -825.
-
Gallagher, R.T., Hawkes, A.D., Steyn, P.S. and Vleggaar, R., 1984. Tremorgenic neurotoxins from perennial ryegrass causing ryegrass staggers disorder of livestock: structure elucidation of lolitrem B. Journal of the Chemical Society / Chemical Communications 9: 614-616.
'Tremorgenic neurotoxins from perennial ryegrass causing ryegrass staggers disorder of livestock: structure elucidation of lolitrem B ' () 9 Journal of the Chemical Society / Chemical Communications : 614 -616.
-
Gilruth, J., 1906. Meningo-encephalitis (stomach staggers) in horses, cattle and sheep. Annual Report of the New Zealand Department of Agriculture Appendix VIII. Division of Veterinary Science, Wellington, New Zealand, pp. 293-297.
Annual Report of the New Zealand Department of Agriculture Appendix VIII , () 293 -297.
-
Hannun, Y.A. and Obeid, L.M., 2008. Principles of bioactive lipid signalling: lessons from sphingolipids. Nature Reviews Molecular Cell Biology 9: 139-150.
'Principles of bioactive lipid signalling: lessons from sphingolipids ' () 9 Nature Reviews Molecular Cell Biology : 139 -150.
-
Hovermale, J.T. and Craig, A.M., 2001. Correlation of ergovaline and lolitrem B levels in endophyte-infected perennial ryegrass (Lolium perenne). Journal of Veterinary Diagnostic Investigation 13: 323-327.
'Correlation of ergovaline and lolitrem B levels in endophyte-infected perennial ryegrass (Lolium perenne) ' () 13 Journal of Veterinary Diagnostic Investigation : 323 -327.
-
Huang, D.W., Sherman, B.T. and Lempicki, R.A., 2009a. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature Protocols 4: 44-57.
'Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources ' () 4 Nature Protocols : 44 -57.
-
Huang, D.W., Sherman, B.T. and Lempicki, R.A., 2009b. Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Research 37: 1-13.
'Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists ' () 37 Nucleic Acids Research : 1 -13.
-
Imlach, W.L., Finch, S.C., Dunlop, J. and Dalziel, J.E., 2009. Structural determinants of lolitrems for inhibition of BK large conductance Ca2+-activated K+ channels. European Journal of Pharmacology 605: 36-45.
'Structural determinants of lolitrems for inhibition of BK large conductance Ca2+-activated K+ channels ' () 605 European Journal of Pharmacology : 36 -45.
-
Imlach, W.L., Finch, S.C., Dunlop, J., Meredith, A.L., Aldrich, R.W. and Dalziel, J.E., 2008. The molecular mechanism of ‘ryegrass staggers,’ a neurological disorder of K+ channels. Journal of Pharmacology and Experimental Therapeutics 327: 657-664.
'The molecular mechanism of ‘ryegrass staggers,’ a neurological disorder of K+ channels ' () 327 Journal of Pharmacology and Experimental Therapeutics : 657 -664.
-
Imlach, W.L., Finch, S.C., Zhang, Y., Dunlop, J. and Dalziel, J.E., 2011. Mechanism of action of lolitrem B, a fungal endophyte derived toxin that inhibits BK large conductance Ca2+-activated K+ channels. Toxicon 57: 686-694.
'Mechanism of action of lolitrem B, a fungal endophyte derived toxin that inhibits BK large conductance Ca2+-activated K+ channels ' () 57 Toxicon : 686 -694.
-
Irizarry, R.A., Bolstad, B.M., Collin, F., Cope, L.M., Hobbs, B. and Speed, T.P., 2003. Summaries of Affymetrix GeneChip probe level data. Nucleic Acids Research 31: e15.
'Summaries of Affymetrix GeneChip probe level data ' () 31 Nucleic Acids Research : e15.
-
Johnstone, L.K. and Mayhew, I.G., 2013. Flow-mediated K+ secretion in horses intoxicated with lolitrem B (perennial ryegrass staggers). New Zealand Veterinary Journal 61: 159-164.
'Flow-mediated K+ secretion in horses intoxicated with lolitrem B (perennial ryegrass staggers) ' () 61 New Zealand Veterinary Journal : 159 -164.
-
Johnstone, L.K., Mayhew, I.G. and Fletcher, L.R., 2012. Clinical expression of lolitrem B (perennial ryegrass) intoxication in horses. Equine Veterinary Journal 44: 304-309.
'Clinical expression of lolitrem B (perennial ryegrass) intoxication in horses ' () 44 Equine Veterinary Journal : 304 -309.
-
Kanehisa, M. and Goto, S., 2000. KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Research 28: 27-30.
'KEGG: Kyoto encyclopedia of genes and genomes ' () 28 Nucleic Acids Research : 27 -30.
-
Kitatani, K., Idkowiak-Baldys, J. and Hannun, Y.A., 2008. The sphingolipid salvage pathway in ceramide metabolism and signaling. Cellular Signaling 20: 1010-1018.
'The sphingolipid salvage pathway in ceramide metabolism and signaling ' () 20 Cellular Signaling : 1010 -1018.
-
Luong, A., Hannah, V.C., Brown, M.S. and Goldstein, J.L., 2000. Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins. Journal of Biological Chemistry 275: 26458-26466.
'Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins ' () 275 Journal of Biological Chemistry : 26458 -26466.
-
Malleske, D.T., Rogers, L.K., Velluci, S.M., Young, T.L., Park, M.S., Long, D.W., Welty, S.E., Smith, C.V. and Nelin, L.D., 2006. Hyperoxia increases hepatic arginase expression and ornithine production in mice. Toxicology and Applied Pharmacology 215: 109-117.
'Hyperoxia increases hepatic arginase expression and ornithine production in mice ' () 215 Toxicology and Applied Pharmacology : 109 -117.
-
Mandrup, S., Hummel, R., Ravn, S., Jensen, G., Andreasen, P.H., Gregersen, N., Knudsen, J. and Kristiansen, K., 1992. Acyl-CoA-binding protein/diazepam-binding inhibitor gene and pseudogenes. Journal of Molecular Biology 228: 1011-1022.
'Acyl-CoA-binding protein/diazepam-binding inhibitor gene and pseudogenes ' () 228 Journal of Molecular Biology : 1011 -1022.
-
Mantle, P.G., 1983. Amino acid neurotransmitter release from cerebrocortical synaptosomes of sheep with severe ryegrass staggers in New Zealand. Research in Veterinary Science 34: 373-375.
'Amino acid neurotransmitter release from cerebrocortical synaptosomes of sheep with severe ryegrass staggers in New Zealand ' () 34 Research in Veterinary Science : 373 -375.
-
Mason, R.W., 1968. Axis cylinder degeneration associated with ryegrass staggers in sheep and cattle. Australian Veterinary Journal 44: 428-428.
'Axis cylinder degeneration associated with ryegrass staggers in sheep and cattle ' () 44 Australian Veterinary Journal : 428 -428.
-
Mathur, S., Constable, P.D., Eppley, R.M., Waggoner, A.L., Tumbleson, M.E. and Haschek, W.M., 2001. Fumonisin B1 is hepatotoxic and nephrotoxic in milk-fed calves. Toxicological Sciences 60: 385-396.
'Fumonisin B1 is hepatotoxic and nephrotoxic in milk-fed calves ' () 60 Toxicological Sciences : 385 -396.
-
Moreno-López, B. and González-Forero, D., 2006. Nitric oxide and synaptic dynamics in the adult brain: physiopathological aspects. Reviews in the Neurosciences 17: 309-357.
'Nitric oxide and synaptic dynamics in the adult brain: physiopathological aspects ' () 17 Reviews in the Neurosciences : 309 -357.
-
Muller, F.L., Lustgarten, M.S., Jang, Y., Richardson, A. and Van Remmen, H., 2007. Trends in oxidative aging theories. Free Radical Biology and Medicine 43: 477-503.
'Trends in oxidative aging theories ' () 43 Free Radical Biology and Medicine : 477 -503.
-
Oshino, N., Imai, Y. and Sato, R., 1971. A function of cytochrome b5 in fatty acid desaturation by rat liver microsomes. Journal of Biochemistry 69: 155-167.
'A function of cytochrome b5 in fatty acid desaturation by rat liver microsomes ' () 69 Journal of Biochemistry : 155 -167.
-
Papadopoulos, V. and Brown, A.S., 1995. Role of the peripheral-type benzodiazepine receptor and the polypeptide diazepam binding inhibitor in steroidogenesis. Journal of Steroid Biochemistry and Molecular Biology 53: 103-110.
'Role of the peripheral-type benzodiazepine receptor and the polypeptide diazepam binding inhibitor in steroidogenesis ' () 53 Journal of Steroid Biochemistry and Molecular Biology : 103 -110.
-
Perruchet, C., 1983. Constrained agglomerative hierarchical classification. Pattern Recognition 16: 213-217.
'Constrained agglomerative hierarchical classification ' () 16 Pattern Recognition : 213 -217.
-
Prast, H. and Philippu, A., 2001. Nitric oxide as modulator of neuronal function. Progress in Neurobiology 64: 51-68.
'Nitric oxide as modulator of neuronal function ' () 64 Progress in Neurobiology : 51 -68.
-
Prestidge, R., 1993. Causes and Control of Perennial Ryegrass Staggers in New-Zealand. Agriculture Ecosystems and Environment 44: 283-300.
'Causes and Control of Perennial Ryegrass Staggers in New-Zealand ' () 44 Agriculture Ecosystems and Environment : 283 -300.
-
Schroepfer, G.J., 1982. Sterol biosynthesis. Annual Review of Biochemistry 51: 555-585.
'Sterol biosynthesis ' () 51 Annual Review of Biochemistry : 555 -585.
-
Seiler, A., Schneider, M., Förster, H., Roth, S., Wirth, E.K., Culmsee, C., Plesnila, N., Kremmer, E., Rådmark, O., Wurst, W., Bornkamm, G.W., Schweizer, U. and Conrad, M., 2008. Glutathione peroxidase 4 senses and translates oxidative stress into 12/15 lipoxygenase dependent- and AIF-mediated cell death. Cell Metabolism 8: 237-248.
'Glutathione peroxidase 4 senses and translates oxidative stress into 12/15 lipoxygenase dependent- and AIF-mediated cell death ' () 8 Cell Metabolism : 237 -248.
-
Smyth, G.K., 2004. Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Statistical Applications in Genetics and Molecular Biology 3: 3.
'Linear models and empirical Bayes methods for assessing differential expression in microarray experiments ' () 3 Statistical Applications in Genetics and Molecular Biology : 3.
-
Stockmann-Juvala, H. and Savolainen, K., 2008. A review of the toxic effects and mechanisms of action of fumonisin B1. Human and Experimental Toxicology 27: 799-809.
'A review of the toxic effects and mechanisms of action of fumonisin B1 ' () 27 Human and Experimental Toxicology : 799 -809.
-
Tor-Agbidye, J., Blythe, L.L. and Craig, A.M., 2001. Correlation of endophyte toxins (ergovaline and lolitrem B) with clinical disease: fescue foot and perennial ryegrass staggers. Veterinary and Human Toxicology 43: 140-146.
'Correlation of endophyte toxins (ergovaline and lolitrem B) with clinical disease: fescue foot and perennial ryegrass staggers ' () 43 Veterinary and Human Toxicology : 140 -146.
-
Zeidan, Y.H. and Hannun, Y.A., 2007. Translational aspects of sphingolipid metabolism. Trends in Molecular Medicine 13: 327-336.
'Translational aspects of sphingolipid metabolism ' () 13 Trends in Molecular Medicine : 327 -336.
Supplementary Materials
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 305 | 122 | 15 |
| Gesamttextansichten | 25 | 0 | 0 |
| PDF-Downloads | 3 | 0 | 0 |
Bovine hepatic differential gene expression in response to perennial ryegrass staggers
in World Mycotoxin JournalSearch for other papers by L. Li in
Current site
Google Scholar
PubMed
Search for other papers by D.D. Tanaree in
Current site
Google Scholar
PubMed
Search for other papers by Y. Di in
Current site
Google Scholar
PubMed
Search for other papers by C.T. Estill in
Current site
Google Scholar
PubMed
Search for other papers by J.M. Duringer in
Current site
Google Scholar
PubMed
Search for other papers by L.L. Blythe in
Current site
Google Scholar
PubMed
Search for other papers by A. Galen in
Current site
Google Scholar
PubMed
Search for other papers by S. Livesay in
Current site
Google Scholar
PubMed
Search for other papers by A.M. Craig in
Current site
Google Scholar
PubMed
>‘Perennial ryegrass staggers’ is a neurological condition characterised by muscular tremors and ataxia in livestock that ingest the indole diterpene lolitrem B from endophyte-infected perennial ryegrass. While the neurotoxic mechanism of action of ryegrass staggers has been defined, little is known about metabolic and other molecular processes that lolitrem B may affect in livestock. The objective of this study was to characterise differential gene expression in the liver of animals fed lolitrem B-containing feed over an extended period of time. Eighteen steers were fed one of three rations (n=6/group) containing lolitrem B (247, 1,554 or 2,256 μg/kg (dry matter basis)) over 64 days in a double-blind study. Microarray and confirmatory qPCR were performed to evaluate the hepatic gene expression profile from biopsies taken on days 0 and 64. Widespread perturbation of gene expression was observed in all groups receiving lolitrem B, with a total of 152 differential genes identified (false discovery rate ≤0.05). This suggests that chronic exposure to lolitrem B, even at levels below the current threshold of toxicity (2,000 μg/kg lolitrem B), can perturb many genes, biological processes and pathways. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that many of these genes were categorised under lipid/steroid biosynthesis/metabolism and oxidation-reduction. Specifically, genes involved in the biosynthesis pathway of ceramide, a sphingolipid molecule (ACSS2,LASS6 andSCD) and changes in neurosignaling through alteration of nitric oxide synthase activity (ARG1 andGPX4) were up-regulated. Future work should focus on the overall balance between ceramide and its metabolites and antioxidants/oxidants in a variety of body matrices in animals with perennial ryegrass staggers, to determine how these compounds contribute to the overall etiology of this disease.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 305 | 122 | 15 |
| Gesamttextansichten | 25 | 0 | 0 |
| PDF-Downloads | 3 | 0 | 0 |
