Inflammatory bowel diseases (IBD) are chronic inflammatory conditions, characterised by remissions and relapses episodes, whose main manifestations are ulcerative colitis and Crohn's disease. Ulcerative colitis (UC), one of the main forms of IBD, has as standard treatment the use of corticosteroids and anti-inflammatory drugs. The use of antibiotics has been also reported, but the possible adverse effects, such as disturbance of the indigenous microbiota or resistance induction, should be taken into consideration, and thus the use of probiotics emerges as a possible alternative option of treatment. In this study, the oral administration of Bifidobacterium longum subsp. infantis BB-02 was evaluated as a preventive strategy for acute experimental UC induced in female BALB/c mice by ingestion of 3.5% dextran sulphate sodium in drinking water during 7 days. During this time, the daily disease activity index was evaluated, and on the seventh day the animals were euthanised to collect intestines and liver for analysis. Treatment with the probiotic resulted in clinical improvement of the animals. The histological and morphometric analyses showed a reduction of lesions and oedema in the gut, but there was no increase in the production of mucin. The dosage of secretory immunoglobulin A was significantly higher in the colitis group and reduced in the group treated with the probiotic. There was also a reduction in the inflammation of the colon, as demonstrated by a decrease in neutrophils infiltration, and KC/CXCL-1 levels. The intestinal permeability, which is typically increased during the onset of IBD, was also reduced by treatment with probiotic. Based on these data, it can be concluded that the bacterium B. infantis BB-02 has a probiotic potential for the attenuation of UC, but further studies should be conducted to verify the mechanism of protective action of the bacterium.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
Albert, E., Walker, J., Thiesen, A., Churchill, T. and Madsen, K., 2010. Cis-urocanic acid attenuates acute dextran sodium sulphate-induced intestinal inflammation. PLoS ONE 5: e13676.
'Cis-urocanic acid attenuates acute dextran sodium sulphate-induced intestinal inflammation ' () 5 PLoS ONE : e13676.
Anezaki, K., Asakura, H., Honma, T., Ishizuka, K., Funakoshi, K., Tsukada, Y. and Narisawa, R., 1998. Correlations between interleukin-8, and myeloperoxidase or luminol-dependent chemiluminescence in inflamed mucosa of ulcerative colitis. Internal Medicine 37: 253-258.
'Correlations between interleukin-8, and myeloperoxidase or luminol-dependent chemiluminescence in inflamed mucosa of ulcerative colitis ' () 37 Internal Medicine : 253 -258.
Bennedsen, M., Stuer-Lauridsen, B., Danielsen, M. and Johansen, E., 2011. Screening for antimicrobial resistance genes and virulence factors via genome sequencing. Applied and Environmental Microbiology 77: 2785-2787.
'Screening for antimicrobial resistance genes and virulence factors via genome sequencing ' () 77 Applied and Environmental Microbiology : 2785 -2787.
Bibiloni, R., Fedorak, R.N., Tannock, G.W., Madsen, K.L., Gionchetti, P., Campieri, M., De Simone, C. and Sartor, R.B., 2005. VSL#3 probiotic-mixture induces remission in patients with active ulcerative colitis. American Journal of Gastroenterology 100: 1539-1546.
'VSL#3 probiotic-mixture induces remission in patients with active ulcerative colitis ' () 100 American Journal of Gastroenterology : 1539 -1546.
Bin-Nun, A., Bromiker, R., Wilschanski, M., Kaplan, M., Rudensky, B., Caplan, M. and Hammerman, C., 2005. Oral probiotics prevent necrotizing enterocolitis in very low birth weight neonates. Journal of Pediatrics 147: 192-196.
'Oral probiotics prevent necrotizing enterocolitis in very low birth weight neonates ' () 147 Journal of Pediatrics : 192 -196.
Buanne, P., Di Carlo, E., Caputi, L., Brandolini, L., Mosca, M., Cattani, F., Pellegrini, L., Biordi, L., Coletti, G., Sorrentino, C., Fedele, G., Colotta, F., Melillo, G. and Bertini, R., 2007. Crucial pathophysiological role of CXCR2 in experimental ulcerative colitis in mice. Journal of Leukocyte Biology 82: 1239-1246.
'Crucial pathophysiological role of CXCR2 in experimental ulcerative colitis in mice ' () 82 Journal of Leukocyte Biology : 1239 -1246.
Carlson, M., Raab, Y., Peterson, C., Hällgren, R. and Venge, P., 1999. Increased intraluminal release of eosinophil granule proteins EPO, ECP, EPX, and cytokines in ulcerative colitis and proctitis in segmental perfusion. American Journal of Gastroenterology 94: 1876-1883.
'Increased intraluminal release of eosinophil granule proteins EPO, ECP, EPX, and cytokines in ulcerative colitis and proctitis in segmental perfusion ' () 94 American Journal of Gastroenterology : 1876 -1883.
Chen, W., Paulus, B., Shu, D., Wilson, I. and Chadwick, V., 2001. Increased serum levels of eotaxin in patients with inflammatory bowel disease. Scandinavian Journal of Gastroenterology 36: 515-520.
'Increased serum levels of eotaxin in patients with inflammatory bowel disease ' () 36 Scandinavian Journal of Gastroenterology : 515 -520.
Cooper, H.S., Murthy, S.N., Shah, R.S. and Sedergran, D.J., 1993. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Laboratory Investigation 6: 238-249.
'Clinicopathologic study of dextran sulfate sodium experimental murine colitis ' () 6 Laboratory Investigation : 238 -249.
Corrêa, N.B.O., Lima, F.M.L.S., Nicoli, J.R., Péret, L.A.F. and Penna, F.J., 2005. A randomized placebo-controlled trial of Bifidobacterium lactis and Streptococcus thermophilus for prevention of antibiotic-associated diarrhea in infants. Journal of Clinical Gastroenterology 39: 385-389.
'A randomized placebo-controlled trial of Bifidobacterium lactis and Streptococcus thermophilus for prevention of antibiotic-associated diarrhea in infants ' () 39 Journal of Clinical Gastroenterology : 385 -389.
Danese, S. and Fiocchi, C., 2011. Ulcerative colitis. New England Journal of Medicine 365: 1713-1725.
'Ulcerative colitis ' () 365 New England Journal of Medicine : 1713 -1725.
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. Available at: ftp://ftp.fao.org/es/esn/food/wgreport2.pdf
Garcia Vilela, E., De Lourdes De Abreu Ferrari, M., Oswaldo Da Gama Torres, H., Guerra Pinto, A., Carolina Carneiro Aguirre, A., Paiva Martins, F., Marcos Andrade Goulart, E. and Sales Da Cunha, A., 2008. Influence of Saccharomyces boulardii on the intestinal permeability of patients with Crohn's disease in remission. Scandinavian Journal of Gastroenterology 43: 842-848.
'Influence of Saccharomyces boulardii on the intestinal permeability of patients with Crohn's disease in remission ' () 43 Scandinavian Journal of Gastroenterology : 842 -848.
Garrido-Mesa, N., Utrilla, P., Comalada, M., Zorrilla, P., Garrido-Mesa, J., Zarzuelo, A., Rodríguez-Cabezas, M.E. and Gálvez, J., 2005. The association of minocycline and the probiotic Escherichia coli Nissle 1917 results in an additive beneficial effect in a DSS model of reactivated colitis in mice. Biochemical Pharmacology 82: 1891-1900.
'The association of minocycline and the probiotic Escherichia coli Nissle 1917 results in an additive beneficial effect in a DSS model of reactivated colitis in mice ' () 82 Biochemical Pharmacology : 1891 -1900.
Gionchetti, P., Rizzello, F., Helwig, U., Venturi, A., Lammers, K.M., Brigidi, P., Vitali, B., Poggioli, G., Miglioli, M. and Campieri, M., 2003. Prophylaxis of pouchitis onset with probiotic therapy: a double-blind, placebo-controlled trial. Gastroenterology 124: 1202-1209.
'Prophylaxis of pouchitis onset with probiotic therapy: a double-blind, placebo-controlled trial ' () 124 Gastroenterology : 1202 -1209.
Gionchetti, P., Rizzello, F., Venturi, A., Brigidi, P., Matteuzzi, D., Bazzocchi, G., Poggioli, G. and Campieri, M., 2000. Oral bacteriotherapy as maintenance treatment in patients with chronic pouchitis: a double-blind, placebo-controlled trial. Gastroenterology 119: 305-309.
'Oral bacteriotherapy as maintenance treatment in patients with chronic pouchitis: a double-blind, placebo-controlled trial ' () 119 Gastroenterology : 305 -309.
Guerra, P.V.P., Lima, L.N., Souza, T.C., Mazochi, V., Penna, F.J., Silva, A.M., Nicoli, J.R. and Guimarães, E.V., 2011. Pediatric functional constipation treatment with Bifidobacterium-containing yogurt: a crossover, double-blind, controlled trial. World Journal of Gastroenterology 17: 3916-3921.
'Pediatric functional constipation treatment with Bifidobacterium-containing yogurt: a crossover, double-blind, controlled trial ' () 17 World Journal of Gastroenterology : 3916 -3921.
Guslandi, M., 2010. Saccharomyces boulardii plus rifaximin in mesalamine-intolerant ulcerative colitis. Journal of Clinical Gastroenterology 44: 385.
'Saccharomyces boulardii plus rifaximin in mesalamine-intolerant ulcerative colitis ' () 44 Journal of Clinical Gastroenterology : 385.
Guslandi, M., Giollo, P. and Testoni, P.A., 2003. A pilot trial of Saccharomyces boulardii in ulcerative colitis. European Journal of Gastroenterology and Hepatology 15: 697-698.
'A pilot trial of Saccharomyces boulardii in ulcerative colitis ' () 15 European Journal of Gastroenterology and Hepatology : 697 -698.
Guslandi, M., Mezzi, G., Sorghi, M. and Testoni, P.A., 2000. Saccharomyces boulardii in maintenance treatment of Crohn's disease. Digestive Diseases and Sciences 45: 1462-1464.
'Saccharomyces boulardii in maintenance treatment of Crohn's disease ' () 45 Digestive Diseases and Sciences : 1462 -1464.
Jacobs, S.E., Tobin, J.M., Opie, G.F., Donath, S., Tabrizi, S.N., Pirotta, M., Morley, C.J., Garland, S.M. and ProPrems Study Group, 2013. Probiotic effects on late-onset sepsis in very preterm infants: a randomized controlled trial. Pediatrics 132, 1055-1062.
Jadhav, S.R., Shandilya, U.K. and Kansal, V.K., 2013. Exploring the ameliorative potential of probiotic Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum on dextran sodium sulphate induced colitis in mice. Journal of Dairy Research 80: 21-27.
'Exploring the ameliorative potential of probiotic Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum on dextran sodium sulphate induced colitis in mice ' () 80 Journal of Dairy Research : 21 -27.
Jeziorska, M., Haboubi, N., Schofield, P. and Woolley, D.E., 2001. Distribution and activation of eosinophils in inflammatory bowel disease using an improved immunohistochemical technique. Journal of Pathology 194: 484-492.
'Distribution and activation of eosinophils in inflammatory bowel disease using an improved immunohistochemical technique ' () 194 Journal of Pathology : 484 -492.
Kamada, N., Inoue, N., Hisamatsu, T., Okamoto, S., Matsuoka, K., Sato, T., Chinen, H., Hong, K.S., Yamada, T., Suzuki, Y., Suzuki, T., Watanabe, N., Tsuchimoto, K. and Hibi, T., 2005. Nonpathogenic Escherichia coli strain Nissle1917 prevents murine acute and chronic colitis. Inflammatory Bowel Diseases 11: 455-463.
'Nonpathogenic Escherichia coli strain Nissle1917 prevents murine acute and chronic colitis ' () 11 Inflammatory Bowel Diseases : 455 -463.
Keshavarzian, A., Fusunyan, R.D., Jacyno, M., Winship, D., MacDermott, R.P. and Sanderson, I.R., 1999. Increased interleukin-8 (IL-8) in rectal dialysate from patients with ulcerative colitis: evidence for a biological role for IL-8 in inflammation of the colon. American Journal of Gastroenterology 94: 704-712.
'Increased interleukin-8 (IL-8) in rectal dialysate from patients with ulcerative colitis: evidence for a biological role for IL-8 in inflammation of the colon ' () 94 American Journal of Gastroenterology : 704 -712.
Lampinen, M., Carlson, M., Sangfelt, P., Taha, Y., Thörn, M., Lööf, L., Raab, Y. and Venge, P., 2001. IL-5 and TNF-? participate in recruitment of eosinophils to intestinal mucosa in ulcerative colitis. Digestive Diseases and Sciences 46: 2004-2009.
'IL-5 and TNF-? participate in recruitment of eosinophils to intestinal mucosa in ulcerative colitis ' () 46 Digestive Diseases and Sciences : 2004 -2009.
Macpherson, A.J. and Harris, N.L., 2004. Interactions between commensal bacteria and the immune system. Nature Reviews Immunology 4: 478-485.
'Interactions between commensal bacteria and the immune system ' () 4 Nature Reviews Immunology : 478 -485.
Martins, F.S., Silva, A.A., Vieira, A.T., Barbosa, F.H., Arantes, R.M., Teixeira, M.M. and Nicoli, J.R., 2009. Comparative study of Bifidobacterium animalis, Escherichia coli, Lactobacillus casei and Saccharomyces boulardii probiotic properties. Archives of Microbiology 191: 623-630.
'Comparative study of Bifidobacterium animalis, Escherichia coli, Lactobacillus casei and Saccharomyces boulardii probiotic properties ' () 191 Archives of Microbiology : 623 -630.
Martins, F.S., Vieira, A.T., Elian, S.D., Arantes, R.M., Tiago, F.C., Sousa, L.P., Araújo, H.R., Pimenta, P.F., Bonjardim, C.A., Nicoli, J.R. and Teixeira, M.M., 2013. Inhibition of tissue inflammation and bacterial translocation as one of the protective mechanisms of Saccharomyces boulardii against Salmonella infection in mice. Microbes and Infection 15: 270-279.
'Inhibition of tissue inflammation and bacterial translocation as one of the protective mechanisms of Saccharomyces boulardii against Salmonella infection in mice ' () 15 Microbes and Infection : 270 -279.
McGuckin, M.A., Lindén, S.K., Sutton, P. and Florin, T.H., 2011. Mucin dynamics and enteric pathogens. Nature Reviews Microbiology 9: 265-278.
'Mucin dynamics and enteric pathogens ' () 9 Nature Reviews Microbiology : 265 -278.
Natsui, M., Kawasaki, K., Takizawa, H., Hayashi, S.I., Matsuda, Y., Sugimura, K., Seki, K., Narisawa, R., Sendo, F. and Asakura, H., 1997. Selective depletion of neutrophils by a monoclonal antibody. RP-3, suppresses dextran sulphate sodium-induced colitis in rats. Journal of Gastroenterology and Hepatology 12: 801-808.
'Selective depletion of neutrophils by a monoclonal antibody. RP-3, suppresses dextran sulphate sodium-induced colitis in rats ' () 12 Journal of Gastroenterology and Hepatology : 801 -808.
Ofek Shlomai, N., Deshpande, G., Rao, S. and Patole, S., 2014. Probiotics for preterm neonates: what will it take to change clinical practice? Neonatology 105: 64-70.
'Probiotics for preterm neonates: what will it take to change clinical practice? ' () 105 Neonatology : 64 -70.
Osman, N., Adawi, D., Ahrne, S., Jeppsson, B. and Molin, G., 2004. Modulation of the effect of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium. Digestive Diseases and Sciences 49: 320-327.
'Modulation of the effect of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium ' () 49 Digestive Diseases and Sciences : 320 -327.
Perše, M. and Cerar, A., 2012. Dextran sodium sulphate colitis mouse model: traps and tricks. Journal of Biomedicine and Biotechnology 2012: 718617.
'Dextran sodium sulphate colitis mouse model: traps and tricks ' () 2012 Journal of Biomedicine and Biotechnology : 718617.
Plein, K. and Hotz, J., 1993. Therapeutic effects of Saccharomyces boulardii on mild residual symptoms in a stable phase of Crohn's disease with special respect to chronic diarrhea – a pilot study. Zeitschrift für Gastroenterologie 31: 129-134.
'Therapeutic effects of Saccharomyces boulardii on mild residual symptoms in a stable phase of Crohn's disease with special respect to chronic diarrhea – a pilot study ' () 31 Zeitschrift für Gastroenterologie : 129 -134.
Raab, Y., Fredens, K., Gerdin, B. and Hällgren, R., 1998. Eosinophil activation in ulcerative colitis: studies on mucosal release and localization of eosinophil granule constituents. Digestive Diseases and Sciences 43: 1061-1070.
'Eosinophil activation in ulcerative colitis: studies on mucosal release and localization of eosinophil granule constituents ' () 43 Digestive Diseases and Sciences : 1061 -1070.
Rembacken, B.J., Snelling, A.M., Hawkey, P.M., Chalmers, D.M. and Axon, A.T., 1999. Non-pathogenic Escherichia coli versus mesalazine for the treatment of ulcerative colitis: a randomised trial. The Lancet 354: 635-639.
'Non-pathogenic Escherichia coli versus mesalazine for the treatment of ulcerative colitis: a randomised trial ' () 354 The Lancet : 635 -639.
Sangfelt, P., Carlson, M., Thörn, M., Lööf, L. and Raab, Y., 2001. Neutrophil and eosinophil granule proteins as markers of response to local prednisolone treatment in distal ulcerative colitis and proctitis. American Journal of Gastroenterology 96: 1085-1090.
'Neutrophil and eosinophil granule proteins as markers of response to local prednisolone treatment in distal ulcerative colitis and proctitis ' () 96 American Journal of Gastroenterology : 1085 -1090.
Scapini, P., Lapinet-Vera, J.A., Gasperini, S., Calzetti, F., Bazzoni, F. and Cassatella, M.A., 2000. The neutrophil as a cellular source of chemokines. Immunological Reviews 177: 195-203.
'The neutrophil as a cellular source of chemokines ' () 177 Immunological Reviews : 195 -203.
Sociedade Brasileira da Ciência em Animais de Laboratório/Colégio Brasileiro de Experimentação Animal (SBCAL/COBEA), 2006. Legislação e Ética. Available at: http://www.cobea.org.br
Strath, M., Warren, D.J. and Sanderson, C.J., 1985. Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors. Journal of Immunological Methods 83: 209-215.
'Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors ' () 83 Journal of Immunological Methods : 209 -215.
Uguccioni, M., Gionchetti, P., Robbiani, D.F., Rizzello, F., Peruzzo, S., Campieri, M. and Baggiolini, M., 1999. Increased expression of IP-10, IL-8, MCP-1, and MCP-3 in ulcerative colitis. American Journal of Pathology 155: 331-336.
'Increased expression of IP-10, IL-8, MCP-1, and MCP-3 in ulcerative colitis ' () 155 American Journal of Pathology : 331 -336.
Ukena, S.N., Singh, A., Dringenberg, U., Engelhardt, R., Seidler, U., Hansen, W., Bleich, A., Bruder, D., Franzke, A., Rogler, G., Suerbaum, S., Buer, J., Gunzer, F. and Westendorf, A.M., 2007. Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity. PLoS ONE 2: e1308.
'Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity ' () 2 PLoS ONE : e1308.
Vieira, A.T., Fagundes, C.T., Alessandri, A.L., Castor, M.G., Guabiraba, R., Borges, V.O., Silveira, K.D., Vieira, E.L., Gonçalves, J.L., Silva, T.A., Deruaz, M., Proudfoot, A.E., Sousa, L.P. and Teixeira, M.M., 2009. Treatment with a novel chemokine-binding protein or eosinophil lineage-ablation protects mice from experimental colitis. American Journal of Pathology 175: 2382-2391.
'Treatment with a novel chemokine-binding protein or eosinophil lineage-ablation protects mice from experimental colitis ' () 175 American Journal of Pathology : 2382 -2391.
Vieira, A.T., Pinho, V., Lepsch, L.B., Scavone, C., Ribeiro, I.M., Tomassini, T., Ribeiro-dos-Santos, R., Soares, M.B., Teixeira, M.M. and Souza, D.G., 2005. Mechanisms of the anti-inflammatory effects of the natural secosteroids physalins in a model of intestinal ischaemia and reperfusion injury. British Journal of Pharmacology 146: 244-251.
'Mechanisms of the anti-inflammatory effects of the natural secosteroids physalins in a model of intestinal ischaemia and reperfusion injury ' () 146 British Journal of Pharmacology : 244 -251.
Vieira, A.T., Teixeira, M.M. and Martins, F.S., 2013. The role of probiotics and prebiotics in inducing gut immunity. Frontiers in Immunology 4: 445.
'The role of probiotics and prebiotics in inducing gut immunity ' () 4 Frontiers in Immunology : 445.
Wirtz, S., Neufert, C., Weigmann, B. and Neurath, M.F., 2007. Chemically induced mouse models of intestinal inflammation. Nature Protocols 2: 541-546.
'Chemically induced mouse models of intestinal inflammation ' () 2 Nature Protocols : 541 -546.
Xavier, R.J. and Podolsky, D.K., 2007. Unravelling the pathogenesis of inflammatory bowel disease. Nature 448: 427-434.
'Unravelling the pathogenesis of inflammatory bowel disease ' () 448 Nature : 427 -434.
Yan, Y., Kolachala, V., Dalmasso, G., Nguyen, H., Laroui, H., Sitaraman, S.V. and Merlin, D., 2009. Temporal and spatial analysis of clinical and molecular parameters in dextran sodium sulfate induced colitis. PLoS ONE 4: e6073.
'Temporal and spatial analysis of clinical and molecular parameters in dextran sodium sulfate induced colitis ' () 4 PLoS ONE : e6073.
Yang, S.K., Choi, M.S., Kim, O.H., Myung, S.J., Jung, H.Y., Hong, W.S., Kim, J.H. and Min, Y.I., 2002. The increased expression of an array of C-X-C and C-C chemokines in the colonic mucosa of patients with ulcerative colitis: regulation by corticosteroids. American Journal of Gastroenterology 97: 126-132.
'The increased expression of an array of C-X-C and C-C chemokines in the colonic mucosa of patients with ulcerative colitis: regulation by corticosteroids ' () 97 American Journal of Gastroenterology : 126 -132.
Zyrek, A.A., Cichon, C., Helms, S., Enders, C., Sonnenborn, U. and Schmidt, M.A., 2007. Molecular mechanisms underlying the probiotic effects of Escherichia coli Nissle 1917 involve ZO-2 and PKCzeta redistribution resulting in tight junction and epithelial barrier repair. Cellular Microbiology 9: 804-816.
'Molecular mechanisms underlying the probiotic effects of Escherichia coli Nissle 1917 involve ZO-2 and PKCzeta redistribution resulting in tight junction and epithelial barrier repair ' () 9 Cellular Microbiology : 804 -816.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 839 | 268 | 18 |
| Full Text Views | 39 | 7 | 0 |
| PDF Views & Downloads | 46 | 12 | 0 |
Inflammatory bowel diseases (IBD) are chronic inflammatory conditions, characterised by remissions and relapses episodes, whose main manifestations are ulcerative colitis and Crohn's disease. Ulcerative colitis (UC), one of the main forms of IBD, has as standard treatment the use of corticosteroids and anti-inflammatory drugs. The use of antibiotics has been also reported, but the possible adverse effects, such as disturbance of the indigenous microbiota or resistance induction, should be taken into consideration, and thus the use of probiotics emerges as a possible alternative option of treatment. In this study, the oral administration of Bifidobacterium longum subsp. infantis BB-02 was evaluated as a preventive strategy for acute experimental UC induced in female BALB/c mice by ingestion of 3.5% dextran sulphate sodium in drinking water during 7 days. During this time, the daily disease activity index was evaluated, and on the seventh day the animals were euthanised to collect intestines and liver for analysis. Treatment with the probiotic resulted in clinical improvement of the animals. The histological and morphometric analyses showed a reduction of lesions and oedema in the gut, but there was no increase in the production of mucin. The dosage of secretory immunoglobulin A was significantly higher in the colitis group and reduced in the group treated with the probiotic. There was also a reduction in the inflammation of the colon, as demonstrated by a decrease in neutrophils infiltration, and KC/CXCL-1 levels. The intestinal permeability, which is typically increased during the onset of IBD, was also reduced by treatment with probiotic. Based on these data, it can be concluded that the bacterium B. infantis BB-02 has a probiotic potential for the attenuation of UC, but further studies should be conducted to verify the mechanism of protective action of the bacterium.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 839 | 268 | 18 |
| Full Text Views | 39 | 7 | 0 |
| PDF Views & Downloads | 46 | 12 | 0 |