Evaluation of 1-deoxynojirimycin in Thai silkworm pupae and its antidiabetic effects in vitro and in vivo
In: Journal of Insects as Food and FeedBiomedicine Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand
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Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Kantarawichai, Maha Sarakham 44150, Thailand
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Abstract
This study evaluated the in vitro and in vivo antidiabetic potential of silkworm pupae (Bombyx mori) extracts from two indigenous Thai varieties, Samrong and Nangnoi, marking the first report quantifying 1-deoxynojirimycin (DNJ) in silkworm pupae. DNJ levels were 45 and 53 mg/g (dry weight) in Samrong and Nangnoi, respectively. Samrong exhibited significantly higher total phenolic (19 mg GAE/g dw) and flavonoid (10 mg QE/g dw) contents than Nangnoi, corresponding to superior antioxidant activity. Both extracts showed α-amylase inhibition (IC50 ≈ 0.40 mg/ml), while Nangnoi demonstrated markedly stronger α-glucosidase inhibition compared to Samrong and the standard drug acarbose. In vivo administration (500 mg/kg for 28 days) in streptozotocin-induced diabetic rats resulted in increased body weight (31% for Samrong, 28% for Nangnoi) and significant reductions in fasting blood glucose (52 and 48%, respectively). Histological analysis revealed pancreatic islet regeneration and improved architecture. These findings highlight the potential of DNJ in silkworm pupae as a natural therapeutic option for diabetes management.
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Evaluation of 1-deoxynojirimycin in Thai silkworm pupae and its antidiabetic effects in vitro and in vivo
In: Journal of Insects as Food and FeedBiomedicine Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand
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Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Kantarawichai, Maha Sarakham 44150, Thailand
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Abstract
This study evaluated the in vitro and in vivo antidiabetic potential of silkworm pupae (Bombyx mori) extracts from two indigenous Thai varieties, Samrong and Nangnoi, marking the first report quantifying 1-deoxynojirimycin (DNJ) in silkworm pupae. DNJ levels were 45 and 53 mg/g (dry weight) in Samrong and Nangnoi, respectively. Samrong exhibited significantly higher total phenolic (19 mg GAE/g dw) and flavonoid (10 mg QE/g dw) contents than Nangnoi, corresponding to superior antioxidant activity. Both extracts showed α-amylase inhibition (IC50 ≈ 0.40 mg/ml), while Nangnoi demonstrated markedly stronger α-glucosidase inhibition compared to Samrong and the standard drug acarbose. In vivo administration (500 mg/kg for 28 days) in streptozotocin-induced diabetic rats resulted in increased body weight (31% for Samrong, 28% for Nangnoi) and significant reductions in fasting blood glucose (52 and 48%, respectively). Histological analysis revealed pancreatic islet regeneration and improved architecture. These findings highlight the potential of DNJ in silkworm pupae as a natural therapeutic option for diabetes management.
| All Time | Past 365 days | Past 30 Days | |
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