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Development of gluten-free cracker using cassava and dough-stage rice flour supplemented with protein from silkworm pupae powder

In: Journal of Insects as Food and Feed
Authors:
P. Hirunyophat Home Economics Program, Department of Applied Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand

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https://orcid.org/0000-0003-0160-671X
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T. Sanphom Home Economics Program, Department of Applied Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand

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https://orcid.org/0000-0003-4482-3271
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C. Wittayapreechakun Home Economics Program, Department of Applied Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand

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C. Hongkulsup Food Science and Technology Program, Department of Applied Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand

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https://orcid.org/0000-0002-0812-4038
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Abstract

This study developed gluten-free crackers using composite flours of cassava flour (CF) and dough-stage rice flour (DRF), supplemented with silkworm pupae powder (SPP) as an alternative protein source. Various CF:DRF ratios (100:0, 75:25, 50:50, 25:75, 0:100) were assessed for their textural and sensory properties, with the 75:25 ratio identified as optimal based on crispness and consumer acceptability comparable to wheat-based controls. This optimal formulation was further enriched with SPP at 5–20% to enhance nutritional value. All formulations maintained their moisture content within industry standards. Increasing SPP levels led to a darker cracker colour and significantly raised protein content, approaching values comparable to wheat crackers. However, SPP concentrations above 15% negatively affected texture and sensory acceptance. The optimised formulation (CF:DRF 75:25 with 10% SPP) exhibited significantly increased crude fibre (1.26 g/100 g), calcium (0.17 g/100 g), vitamin B1 (0.47 mg/100 g), and vitamin B2 (0.67 mg/100 g), while maintaining acceptable protein content (8.36 g/100 g) and sensory characteristics. These results demonstrate the potential of combining underutilised local ingredients and insect protein to produce nutritionally enhanced gluten-free products and promote sustainable food systems.

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